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01-09-2017 | Original Article

The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in diabetic rats

Authors: Arash Esfandiari, Mohsen Roustaei, Ehsan Haghighifarjam

Published in: Comparative Clinical Pathology | Issue 5/2017

Abstract

Diabetic retinopathy is a metabolic disease and oxidative stress-associated diabetic disorder. The antioxidant plants are important in the treatment of diabetic mellitus. The main purpose of this study was to determine the effect of Amygdalus scoparia on anti-diabetic and antioxidant activity in the photoreceptor layer of diabetic retina. Thirty rats were housed and divided into three groups of 10 rats each. Group I: control group; group II: Diabetic group (induced by IP injection of 50 mg/kg of stereptozotocin); group III: diabetic receiving 200 mg/kg A. scoparia extract 1 month before and 2 months after diabetic-induced. At the end of experiment, the eyes were removed and fixed in glutaraldehyde. Then, the routine preparation of tissue in transmission electron microscope was done. The semi-thin and ultrathin sections were stained with toluidine blue, uranyl acetate, and lead citrate, respectively. Then, all sections were evaluated by light and transmission electron microscopes. Result showed that the major signs of pathology such as outer segment loss, large vacuoles, and disorganization in inner segment, pyknotic, karyolysis, and karyorrhexis were seen in diabetic group. But, the minor signs of pathology such as small vacuole and pyknotic nuclei were obvious in amygdulus scoparia treatment group. Our findings revealed reduction of the damage in A. scoparia group which might have antioxidant activity and caused blood glucose to be reduced in diabetic group.

Abo KA, Fred-jaiyesimi AA, Jaiyesimi AEA (2008) Ethnobotanical studies of medicinal plants used in the management of diabetes mellitus in south western Nigeria. J Ethnopharmacol 115:67–71. doi:10.1016/j.jep.2007.09.005 CrossRefPubMed

Amin A, Lotfi M, Shafiullah M, Adeghate E (2006) The protective effect of Tribulus terrestris in diabetes. Ann N Y Acad Sci 1084(1):391–401. doi:10.1196/annals.1372.005 CrossRefPubMed

Andreyev AY, Kushnareva YE, Strakov AA (2005) Mitochondrial metabolism of reactive oxygen species. Biochemistry (Mosc) journal 70:200–214CrossRef

Bonnard C, Durand A, Peyrol S, Chanseaume E et al (2008) Mitochondrial dysfunction results from oxidative stress in the skeletal muscle of diet-induced insulin-resistant mice. J Clin Invest 118(2):789–800PubMedPubMedCentral

Dallak M, Al-khateeb M, Al-hashem F, Bashir N, Abbas M et al (2009) In vivo, acute, normo-hypoglycemic, antihyperglycemic, insulin tropic actions of orally administered ethanol extract of Citrullus colocynthis (L.) schrab pulp. Am J Biochem Biotechnol 5:118–125. doi:10.3844/ajbbsp.2009.118.125 CrossRef

Eddouks M, Lemhadri A, Michel JB (2004) Caraway and caper: potential anti-hyperglycaemic plants in diabetic rats. J Ethnopharmacol 94(1):143–148. doi:10.1016/j.jep.2004.05.006 CrossRefPubMed

Eidia A, Eidib M, Esmaeilia E (2006) Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin-induced diabetic rats. Phytomedicine 13:624–629CrossRef

Farhoosh R, Tavakoli J (2008) Physicochemical properties of kernel oil from Amygdalus scoparia growing wild in Iran. J Food Lipids 15(4):433–443. doi:10.1111/j.1745-4522.2008.00131.x CrossRef

Goodarzi A, Esfandiari A, Dehghansheibani A (2014) Ultra structural study on the photoreceptor layer in streptozotocin-induced diabetic rats. Comp Clin Path 23(5):1293–1297. doi:10.1007/s00580-013-1777-6 CrossRef

Hashemnia M, Nikousefat Z, Yazdani-rostam M (2015) Antidiabetic effect of Pistacia atlantica and Amygdalus scoparia in streptozotocin-induced diabetic mice. Comp Clin Path 24(6):1301–1306. doi:10.1007/s00580-015-2068-1 CrossRef

Hindustan AA, Chitta SK, Poorna SNM, Udaya BT, Ravindra BV, Vamsi MG (2010) Traditional Indian herbs used for diabetes. JITPS 1:69–78

Jelodar GA, Maleki M, Motadayen SS (2005) Effect of fenugreek, onion and garlic on blood glucose and histopathology of pancreas of alloxan-induced diabetic rats. Indian J Med Sci 52:54–69

Jyothi MJ, Avinash Kumar G, Rajasekhar (2011) A review on diabetic rethinopathy. Int J of Pharmacol Research 1(1):1–10

Lin MT, Beal MF (2006) Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 443(7113):787–795CrossRefPubMed

Nakhaee AR, Bokeian M, Saravani M, Farhangi A, Akbarzadeh A (2009) Attenuation of oxidative stress in streptozotocin-induced diabetic rats by Eucalyptus globulus. Indian J Clin Biochem 24(4):419–425. doi:10.1007/s12291-009-0075-1 CrossRefPubMedPubMedCentral

Oryan A, Hashemnia M, Hamidi AR, Mohamadalipur A (2014) Effects of hydro-ethanol extract of Citrullus colocynthis on blood glucose levels and pathology of organs in alloxan-induced diabetic rats. Asian Pac J Trop Dis 4(2):125–130CrossRefPubMedCentral

Park SH, Park JW, Park SJ, Kim KY, Chung JW, Chun MH, Oh SJ (2003) Apoptotic death of photoreceptors in the streptozotocin-induced diabetic rat retina. Diabetologia 46(9):1260–1268. doi:10.1007/s00125-003-1177-6 CrossRefPubMed

Particia VT (2008) The Calam/Acmal Standards of Veterinary Care and laboratory animal welfare. Can Vet J Jan 49:86–88

Rajalakshmi M, Eliza J, Priya CE, Nirmala A, Daisy P (2009) Antidiabetic properties of Tinospora cordifolia stem extracts on streptozotocin- induced diabetic rats. Afr J Pharm Pharmacol 3(5):171–180

Renu AK, Chan PS (2007) Oxidative stress and diabetic retinopathy. Exp Diabetes Res journal 2007:1–12. doi:10.1155/2007/43603

Saberi M, Gholami S (2012) An investigation on the effects of the Aloe Vera extract on the thickness of the retina in male diabetic rats. Iran J Vet Res 13(4):296–302

Thomson M, Al-amin ZM, Al-qattan KK, Shaban LH, Muslim A (2007) Antidiabetic and hypolipidemic properties of garlic Allium sativum in streptozotocin-induced rats. Int J Diabetes & Metabolism 15:108–115

Williams DR, Anthony P, Young RG (1994) Interpreting hospital admissions data across the Korner divide: the example of diabetes in the north west region. Diabet Med 11(2):166–169. doi:10.1111/j.1464-5491.1994.tb02014.x CrossRefPubMed

Title: The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in diabetic rats
Authors: Arash Esfandiari
Mohsen Roustaei
Ehsan Haghighifarjam
Publication date: 01-09-2017
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 5/2017
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-017-2479-2

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The effects of Amygdalus scoparia extract on ultrastructure changes of photoreceptor layer in diabetic rats

Abstract

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Abstract

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