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01-02-2016 | Original Article

The effect of laser therapy on the expression of osteocalcin and osteopontin after tooth extraction in rats treated with zoledronate and dexamethasone

Authors: Giovanni Mergoni, Paolo Vescovi, Roberto Sala, Elisabetta Merigo, Pietro Passerini, Roberta Maestri, Domenico Corradi, Paolo Govoni, Samir Nammour, Massimiliano G. Bianchi

Published in: Supportive Care in Cancer | Issue 2/2016

Abstract

Purpose

Laser therapy has been used for the prevention and management of medication-related ostenecrosis of the jaw (MRONJ). The aim of this paper was to investigate the action of laser therapy on extraction socket healing in rats in conditions at risk for MRONJ, evaluating the expression of markers of bone metabolism.

Methods

Thirty male Sprague–Dawley rats were divided in four groups: control group (C, n = 5), laser group (L, n = 5), treatment group (T, n = 10), and treatment plus laser group (T + L, n = 10). Rats of group T and T + L received zoledronate 0.1 mg/kg and dexamethasone 1 mg/kg every 2 days for 10 weeks. Rats of group C and L were infused with vehicle. After 9 weeks, the left maxillary molars were extracted in all rats. Rats of groups L and T + L received laser therapy (Nd:YAG, 1064 nm, 1.25 W, 15 Hz, 5 min, 14.37 J/cm²) in the socket area at days 0, 2, 4, and 6 after surgery. Western blot analysis was performed to evaluate the alveolar expression of osteopontin (OPN) and osteocalcin (OCN) 8 days after extraction.

Results

Rats of groups L and T + L showed a significant higher expression of OCN compared to rats of groups C and T (+348 and +400 %, respectively; P = 0.013 and P = 0.002, respectively). The expression of OPN did not show significant differences among the different groups.

Conclusions

Our findings suggest that laser irradiation after tooth extraction can promote osteoblast differentiation, as demonstrated by the higher expression of OCN. Thus, laser irradiation could be considered a way to improve socket healing in conditions at risk for MRONJ development.

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Title: The effect of laser therapy on the expression of osteocalcin and osteopontin after tooth extraction in rats treated with zoledronate and dexamethasone
Authors: Giovanni Mergoni
Paolo Vescovi
Roberto Sala
Elisabetta Merigo
Pietro Passerini
Roberta Maestri
Domenico Corradi
Paolo Govoni
Samir Nammour
Massimiliano G. Bianchi
Publication date: 01-02-2016
Publisher: Springer Berlin Heidelberg
Published in: Supportive Care in Cancer / Issue 2/2016
Print ISSN: 0941-4355
Electronic ISSN: 1433-7339
DOI: https://doi.org/10.1007/s00520-015-2847-x

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