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01-02-2020 | Review

Kidney and organoid single-cell transcriptomics: the end of the beginning

Authors: Parker C. Wilson, Benjamin D. Humphreys

Published in: Pediatric Nephrology | Issue 2/2020

Abstract

Single-cell RNA sequencing (scRNA-seq) technologies are increasingly being applied to reveal cellular heterogeneity in kidney development and disease. In just the last year, multiple scRNA-seq datasets have been generated from kidney organoids, developing mouse and human kidney, adult kidney, and kidney cancer. The data generated enables a much deeper understanding of biological processes within and between cells. It has also elucidated unforeseen cell lineage relationships, defined the presence of off-target cell types in kidney organoids, and revealed a diverse inflammatory response in a human kidney allograft undergoing rejection. This review summarizes the recent rapid progress in scRNA-seq of the kidney and outlines future directions for single-cell technologies as applied to the kidney.

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Title: Kidney and organoid single-cell transcriptomics: the end of the beginning
Authors: Parker C. Wilson
Benjamin D. Humphreys
Publication date: 01-02-2020
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Nephrology / Issue 2/2020
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-018-4177-y

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Kidney and organoid single-cell transcriptomics: the end of the beginning

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