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Pediatric Nephrology

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Open Access 01-11-2012 | Original Article

Urinary cytokine profiles in unilateral congenital hydronephrosis

Authors: Katarzyna Taranta-Janusz, Anna Wasilewska, Wojciech Dębek, Marlena Waszkiewicz-Stojda

Published in: Pediatric Nephrology | Issue 11/2012

Abstract

Background

We aimed to evaluate possible clinical application of urinary monocyte chemotactic protein-1 (MCP-1), osteopontin (OPN), and regulated upon activation, normal T-cell expressed and secreted (RANTES) chemokine as useful noninvasive markers in children with congenital hydronephrosis (HN) caused by ureteropelvic junction obstruction (UPJO).

Methods

The study cohort consisted of surgical cases (study group 1), comprising 15 children with severe HN who required surgery (median age 1.03 years), conservative cases (study group 2), comprising 21 patients with mild, nonobstructive HN, and control group, comprising 19 healthy children. All children had normal renal function. Urinary (u) concentrations of MCP-1, OPN, and RANTES were measured using immunoenzymatic enzyme-linked immunosorbent assay (ELISA) commercial kits and were expressed in nanograms per milligram creatinine. Increased levels of MCP-1 and OPN were found in children with HN in comparison with study group 2 and controls (p < 0.05). UMCP-1/Cr correlated with half-time (T_1/2) of the elimination phase of tracer excretion of technetium-99m-mercaptoacetyltriglycine (^99mTc-MAG-3) (p < 0.05).

Results

Receiver operator characteristic (ROC) analyses revealed good diagnostic profile for uMCP-1 only in identifying children (<40 %) with abnormal differential renal function (DRF) [area under the curve (AUC) 0.862], and in detecting kidney injury in all examined children (AUC 0.704).

Conclusions

Additional studies with larger number of patients are required to confirm a potential application of uMCP-1 as a promising parameter for early identification of kidney obstruction.

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Title: Urinary cytokine profiles in unilateral congenital hydronephrosis
Authors: Katarzyna Taranta-Janusz
Anna Wasilewska
Wojciech Dębek
Marlena Waszkiewicz-Stojda
Publication date: 01-11-2012
Publisher: Springer-Verlag
Published in: Pediatric Nephrology / Issue 11/2012
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI: https://doi.org/10.1007/s00467-012-2230-9

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Urinary cytokine profiles in unilateral congenital hydronephrosis

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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