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Surgical Endoscopy

Published in:

01-06-2014

CO₂ pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model

Authors: Toru Kusano, Tsuyoshi Etoh, Masafumi Inomata, Norio Shiraishi, Seigo Kitano

Published in: Surgical Endoscopy | Issue 6/2014

Abstract

Background

Secretory immunoglobulin A (s-IgA) plays an important role in both gut and systemic immunity. This study aimed to investigate the production of s-IgA resulting from a CO₂ pneumoperitoneum compared with a laparotomy.

Methods

Using enzyme-linked immunosorbent assays, s-IgA in stool, malondialdehyde (MDA), and Toll-like receptor 4 (TLR4) in the ileal tissue were evaluated as markers for gut and systemic immune responses in an animal model. The rats were randomly divided into (i) anesthesia-only as the control group; (ii) laparotomy-only as the open group; and (iii) CO₂ pneumoperitoneum-only as the pneumoperitoneum group. To evaluate the gut immune system in a time-dependent manner, each group was further divided into short- and long-time subgroups.

Results

s-IgA levels did not increase in the open group but significantly increased in the pneumoperitoneum group compared with the control group (p < 0.05). In addition, s-IgA levels in the long-time subgroup significantly increased compared with the short-time subgroup of the pneumoperitoneum group (p < 0.05). TLR4 levels steeply and gradually increased in the open and pneumoperitoneum groups, respectively. MDA levels in the pneumoperitoneum group increased during the early phase and were significantly higher than those in the open group at 24 h (p < 0.05).

Conclusions

This study demonstrated that s-IgA levels in stool increased in the pneumoperitoneum group compared with the open group, suggesting that CO₂ pneumoperitoneum may cause transitory damage to the intestinal mucosa.

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Title: CO2 pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model
Authors: Toru Kusano
Tsuyoshi Etoh
Masafumi Inomata
Norio Shiraishi
Seigo Kitano
Publication date: 01-06-2014
Publisher: Springer US
Published in: Surgical Endoscopy / Issue 6/2014
Print ISSN: 0930-2794
Electronic ISSN: 1432-2218
DOI: https://doi.org/10.1007/s00464-013-3408-3

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

CO₂ pneumoperitoneum increases secretory IgA levels in the gut compared with laparotomy in an experimental animal model

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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