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Journal of Cancer Research and Clinical Oncology

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Open Access 01-07-2020 | Cervical Cancer | Original Article – Clinical Oncology

Regulation of LCoR and RIP140 expression in cervical intraepithelial neoplasia and correlation with CIN progression and dedifferentiation

Authors: Tilman L. R. Vogelsang, Elisa Schmoeckel, Christina Kuhn, Thomas Blankenstein, Mina Temelkov, Helene Heidegger, Theresa Maria Kolben, Thomas Kolben, Sven Mahner, Doris Mayr, Udo Jeschke, Aurelia Vattai

Published in: Journal of Cancer Research and Clinical Oncology | Issue 7/2020

Abstract

Purpose

Ligand-dependent corepressor (LCoR) and receptor-interacting protein 140 (RIP140/NRIP1) play an important role in the regulation of multiple oncogenic signaling pathways and the development of cancer. LCoR and RIP140 form a nuclear complex in breast cancer cells and are of prognostic value in further prostate and cervical cancer. The purpose of this study was to analyze the regulation of these proteins in the development of cervical intraepithelial neoplasia (CIN I–III).

Methods

Immunohistochemical analysis was obtained to quantify RIP140 and LCoR expression in formalin-fixed paraffin embedded tissue sections of cervical intraepithelial neoplasia samples. Tissue (n = 94) was collected from patients treated in the Department of Gynecology and Obstetrics, Ludwig-Maximilians-University of Munich, Germany, between 2002 and 2014. Correlations of expression levels with clinical outcome were carried out to assess for prognostic relevance in patients with CIN2 progression. Kruskal–Wallis test and Mann–Whitney U test were used for data analysis.

Results

Nuclear LCoR overexpression correlates significantly with CIN II progression. Nuclear RIP140 expression significantly increases and nuclear LCoR expression decreases with higher grading of cervical intraepithelial neoplasia. Cytoplasmic RIP140 expression is significantly higher in CIN III than in CIN I or CIN II.

Conclusion

A decrease of nuclear LCoR expression in line with an increase of dedifferentiation of CIN can be observed. Nuclear LCoR overexpression correlates with CIN II progression indicating a prognostic value of LCoR in cervical intraepithelial neoplasia. Nuclear and cytoplasmic RIP140 expression increases significantly with higher grading of cervical intraepithelial neoplasia underlining its potential role in the development of pre-cancerous lesions. These findings support the relevance of LCoR and RIP140 in the tumorigenesis indicating a possible role of LCoR and RIP140 as targets for novel therapeutic approaches in cervical intraepithelial neoplasia and cervical cancer.

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Title: Regulation of LCoR and RIP140 expression in cervical intraepithelial neoplasia and correlation with CIN progression and dedifferentiation
Authors: Tilman L. R. Vogelsang
Elisa Schmoeckel
Christina Kuhn
Thomas Blankenstein
Mina Temelkov
Helene Heidegger
Theresa Maria Kolben
Thomas Kolben
Sven Mahner
Doris Mayr
Udo Jeschke
Aurelia Vattai
Publication date: 01-07-2020
Publisher: Springer Berlin Heidelberg
Keywords: Cervical Cancer
Cervical Cancer
Published in: Journal of Cancer Research and Clinical Oncology / Issue 7/2020
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-020-03178-x

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Regulation of LCoR and RIP140 expression in cervical intraepithelial neoplasia and correlation with CIN progression and dedifferentiation

Abstract

Purpose

Methods

Results

Conclusion

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