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Published in: Journal of Cancer Research and Clinical Oncology 2/2017

01-02-2017 | Original Article – Cancer Research

Perilipin 1 is a highly specific marker for adipocytic differentiation in sarcomas with intermediate sensitivity

Authors: Christina C. Westhoff, Janice Mrozinski, Ina Riedel, Hans W. Heid, Roland Moll

Published in: Journal of Cancer Research and Clinical Oncology | Issue 2/2017

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Abstract

Purpose

Liposarcomas are the most common soft tissue sarcomas of adults. The identification of lipoblastic cells in soft tissue sarcomas is mandatory for the diagnosis of most subtypes of liposarcomas but may be difficult in conventional histology. The present study focuses on the expression and possible diagnostic impact of two PAT family proteins, perilipin 1/perilipin and perilipin 2/adipophilin in human liposarcomas.

Methods

Eighty-seven cases of liposarcomas and 30 cases of non-lipomatous sarcomas were investigated immunohistochemically for perilipin 1 and 2 using entire tissue sections. Statistical analyses were performed using appropriate tests.

Results

Most liposarcomas and non-lipomatous sarcomas displayed positivity for perilipin 2. In contrast, while more than two-thirds of liposarcomas presented perilipin 1 positivity, all non-lipomatous sarcomas studied were negative for this marker, with statistical significance (p < 0.001). Perilipin 1 expression increased with adipocytic differentiation of liposarcoma subtypes showing statistical significance (p < 0.001). Non-lipomatous sarcomas demonstrated variable expression levels of perilipin 2. The expression level of perilipin 2 appeared to be correlated with tumor cell degeneration, e.g., through hypoxia.

Conclusions

Perilipin 2 is not well suitable for distinction between liposarcomas and non-lipomatous sarcomas. However, perilipin 1 appeared to be a highly specific marker for liposarcoma and adipocytic differentiation in sarcomas with intermediate sensitivity.
Literature
go back to reference Brasaemle DL, Barber T, Wolins NE, Serrero G, Blanchette-Mackie EJ, Londos C (1997) Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein. J Lipid Res 38(11):2249–2263PubMed Brasaemle DL, Barber T, Wolins NE, Serrero G, Blanchette-Mackie EJ, Londos C (1997) Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein. J Lipid Res 38(11):2249–2263PubMed
go back to reference Dabbs DJ (2014) Diagnostic immunohistochemistry: theranostic and genomic applications, 4th edn. Elsevier/Saunders, Philadelphia Dabbs DJ (2014) Diagnostic immunohistochemistry: theranostic and genomic applications, 4th edn. Elsevier/Saunders, Philadelphia
go back to reference Fletcher CDM, Bridge JA, Hogendoorn PCW, Mertens F (eds) (2013) WHO classification of tumours of soft tissue, 4th edn. World Health Organization Classification of Tumours, Lyon Fletcher CDM, Bridge JA, Hogendoorn PCW, Mertens F (eds) (2013) WHO classification of tumours of soft tissue, 4th edn. World Health Organization Classification of Tumours, Lyon
go back to reference Goldblum JR, Folpe AL, Weiss SW, Enzinger FM (2014) Enzinger and Weiss’s soft tissue tumors, 6th edn. Saunders/Elsevier, Philadelphia Goldblum JR, Folpe AL, Weiss SW, Enzinger FM (2014) Enzinger and Weiss’s soft tissue tumors, 6th edn. Saunders/Elsevier, Philadelphia
go back to reference Katenkamp D (2011) Histological classification of soft tissue tumors and staging according to the TNM system (Histologische Klassifikation von Weichgewebstumoren und Stadieneinteilung gemass TNM-System). Pathologe 32(1):8–13. doi:10.1007/s00292-010-1391-1 CrossRefPubMed Katenkamp D (2011) Histological classification of soft tissue tumors and staging according to the TNM system (Histologische Klassifikation von Weichgewebstumoren und Stadieneinteilung gemass TNM-System). Pathologe 32(1):8–13. doi:10.​1007/​s00292-010-1391-1 CrossRefPubMed
go back to reference Mrozinski J, Schwetlick I, Heid HW, Winter-Simanowski S, Holzbach A, Hinze R, Franke WW, Moll R (1999) Adipophilin and perilipin, two lipid-droplet-associated proteins, in the pathohistological differential diagnosis of human soft tissue sarcomas. P14. J Cancer Res Clin Oncol 125(Suppl):S48–S49 Mrozinski J, Schwetlick I, Heid HW, Winter-Simanowski S, Holzbach A, Hinze R, Franke WW, Moll R (1999) Adipophilin and perilipin, two lipid-droplet-associated proteins, in the pathohistological differential diagnosis of human soft tissue sarcomas. P14. J Cancer Res Clin Oncol 125(Suppl):S48–S49
go back to reference Olsen AM, Eisenberg BL, Kuemmerle NB, Flanagan AJ, Morganelli PM, Lombardo PS, Swinnen JV, Kinlaw WB (2010) Fatty acid synthesis is a therapeutic target in human liposarcoma. Int J Oncol 36(5):1309–1314PubMedPubMedCentral Olsen AM, Eisenberg BL, Kuemmerle NB, Flanagan AJ, Morganelli PM, Lombardo PS, Swinnen JV, Kinlaw WB (2010) Fatty acid synthesis is a therapeutic target in human liposarcoma. Int J Oncol 36(5):1309–1314PubMedPubMedCentral
go back to reference Pandey PR, Liu W, Xing F, Fukuda K, Watabe K (2012) Anti-cancer drugs targeting fatty acid synthase (FAS). Recent Pat Anticancer Drug Discov 7(2):185–197CrossRefPubMed Pandey PR, Liu W, Xing F, Fukuda K, Watabe K (2012) Anti-cancer drugs targeting fatty acid synthase (FAS). Recent Pat Anticancer Drug Discov 7(2):185–197CrossRefPubMed
go back to reference R Development Core Team (2008) R: A language and environment for statistical computing. Austria, Vienna R Development Core Team (2008) R: A language and environment for statistical computing. Austria, Vienna
go back to reference Straub BK, Stoeffel P, Heid H, Zimbelmann R, Schirmacher P (2008) Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47(6):1936–1946. doi:10.1002/hep.22268 CrossRefPubMed Straub BK, Stoeffel P, Heid H, Zimbelmann R, Schirmacher P (2008) Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 47(6):1936–1946. doi:10.​1002/​hep.​22268 CrossRefPubMed
go back to reference Straub BK, Herpel E, Singer S, Zimbelmann R, Breuhahn K, Macher-Goeppinger S, Warth A, Lehmann-Koch J, Longerich T, Heid H, Schirmacher P (2010) Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod Pathol 23(3):480–492. doi:10.1038/modpathol.2009.191 CrossRefPubMed Straub BK, Herpel E, Singer S, Zimbelmann R, Breuhahn K, Macher-Goeppinger S, Warth A, Lehmann-Koch J, Longerich T, Heid H, Schirmacher P (2010) Lipid droplet-associated PAT-proteins show frequent and differential expression in neoplastic steatogenesis. Mod Pathol 23(3):480–492. doi:10.​1038/​modpathol.​2009.​191 CrossRefPubMed
go back to reference Straub BK, Gyoengyoesi B, Koenig M, Hashani M, Pawella LM, Herpel E, Mueller W, Macher-Goeppinger S, Heid H, Schirmacher P (2013) Adipophilin/perilipin-2 as a lipid droplet-specific marker for metabolically active cells and diseases associated with metabolic dysregulation. Histopathology 62(4):617–631. doi:10.1111/his.12038 CrossRefPubMed Straub BK, Gyoengyoesi B, Koenig M, Hashani M, Pawella LM, Herpel E, Mueller W, Macher-Goeppinger S, Heid H, Schirmacher P (2013) Adipophilin/perilipin-2 as a lipid droplet-specific marker for metabolically active cells and diseases associated with metabolic dysregulation. Histopathology 62(4):617–631. doi:10.​1111/​his.​12038 CrossRefPubMed
go back to reference Subirana I, Sanz H, Vila J (2014) Building bivariate tables: the compareGroups package for R. J Stat Softw 57(12):1–16CrossRef Subirana I, Sanz H, Vila J (2014) Building bivariate tables: the compareGroups package for R. J Stat Softw 57(12):1–16CrossRef
go back to reference Wang X, Reape TJ, Li X, Rayner K, Webb CL, Burnand KG, Lysko PG (1999) Induced expression of adipophilin mRNA in human macrophages stimulated with oxidized low-density lipoprotein and in atherosclerotic lesions. FEBS Lett 462(1–2):145–150. doi:10.1016/S0014-5793(99)01521-5 CrossRefPubMed Wang X, Reape TJ, Li X, Rayner K, Webb CL, Burnand KG, Lysko PG (1999) Induced expression of adipophilin mRNA in human macrophages stimulated with oxidized low-density lipoprotein and in atherosclerotic lesions. FEBS Lett 462(1–2):145–150. doi:10.​1016/​S0014-5793(99)01521-5 CrossRefPubMed
Metadata
Title
Perilipin 1 is a highly specific marker for adipocytic differentiation in sarcomas with intermediate sensitivity
Authors
Christina C. Westhoff
Janice Mrozinski
Ina Riedel
Hans W. Heid
Roland Moll
Publication date
01-02-2017
Publisher
Springer Berlin Heidelberg
Published in
Journal of Cancer Research and Clinical Oncology / Issue 2/2017
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-016-2263-8

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