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Journal of Cancer Research and Clinical Oncology
Published in: Journal of Cancer Research and Clinical Oncology 7/2016

01-07-2016 | Original Article – Clinical Oncology

Long noncoding RNA NEAT1 is an unfavorable prognostic factor and regulates migration and invasion in gastric cancer

Authors: Jing-wei Fu, Ying Kong, Xu Sun

Published in: Journal of Cancer Research and Clinical Oncology | Issue 7/2016

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Abstract

Background

Long noncoding RNAs (LncRNAs) have been demonstrated as playing important roles in diverse biological processes including tumorigenesis. However, the clinical significance and biological function of LncRNA nuclear-enriched abundant transcript 1 (NEAT1) in gastric cancer are still unknown. The aim of this study was to identify the role of LncRNA NEAT1 in gastric cancer.

Methods

The expression of LncRNA NEAT1 was detected in gastric cancer samples and cell lines by real-time PCR. The clinical and prognostic significance of LncRNA NEAT1 in gastric cancer patients was analyzed. Furthermore, the biological function of LncRNA NEAT1 on tumor cell growth and mobility were explored through MTT, colony formation, transwell migration, and invasion assays in vitro. The potential mechanism of LncRNA NEAT1 was identified by Western blot.

Results

LncRNA NEAT1 was overexpressed in gastric cancer tissues and cell lines and corrected with clinical stage, histological type, lymph node metastasis, and distant metastasis. Furthermore, patients with high levels of LncRNA NEAT1 had poorer survival than those with lower levels of LncRNA NEAT1. Univariate and multivariate Cox regression analyses showed that LncRNA NEAT1 overexpression was a poor independent prognostic factor for gastric cancer patients. Moreover, knocking down LncRNA NEAT1 expression significantly suppressed the gastric cancer cell migration and invasion in vitro and regulated EMT-associated proteins expression.

Conclusion

LncRNA NEAT1 plays an important role on gastric cancer tumorigenesis and progression and may act as a potential biomarker for therapeutic strategy and prognostic prediction.
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Metadata
Title
Long noncoding RNA NEAT1 is an unfavorable prognostic factor and regulates migration and invasion in gastric cancer
Authors
Jing-wei Fu
Ying Kong
Xu Sun
Publication date
01-07-2016
Publisher
Springer Berlin Heidelberg
Published in
Journal of Cancer Research and Clinical Oncology / Issue 7/2016
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-016-2152-1

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