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Journal of Cancer Research and Clinical Oncology

Published in:

01-01-2013 | Original Paper

Immunotherapy by autologous dendritic cell vaccine in patients with advanced HCC

Authors: Mervat El Ansary, Sherif Mogawer, Samah Abd Elhamid, Sahr Alwakil, Fatma Aboelkasem, Hatem El Sabaawy, Olfat Abdelhalim

Published in: Journal of Cancer Research and Clinical Oncology | Issue 1/2013

Abstract

Background

Dendritic cells (DCs) could be used as potential cellular adjuvant for the production of specific tumor vaccines.

Objectives

Our study was aimed to evaluate the safety and efficacy of autologous pulsed DC vaccine in advanced hepatocellular carcinoma (HCC) patients in comparison with supportive treatment.

Methods

Thirty patients with advanced HCC not suitable for radical or loco-regional therapies were enrolled. Patients were divided into 2 groups, group I consisted of 15 patients received I.D vaccination with mature autologous DCs pulsed ex vivo with a liver tumor cell line lysate. Group II (control group, no. 15) received supportive treatment. One hundred and 4 ml of venous blood were obtained from each patient to generate DCs. DCs were identified by CD80, CD83, CD86 and HLA-DR expressions using flow cytometry. Follow up at 3, and 6 months post injection by clinical, radiological and laboratory assessment was done.

Results

Improvement in overall survival was observed. Partial radiological response was obtained in 2 patients (13.3 %), stable course in 9 patients (60 %) and 4 patients (26.7 %) showed progressive disease (died at 4 months post-injection). Both CD8⁺ T cells and serum interferon gamma were elevated after DCs injection.

Conclusion

Autologous DC vaccination in advanced HCC patients is safe and well tolerate.

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Title: Immunotherapy by autologous dendritic cell vaccine in patients with advanced HCC
Authors: Mervat El Ansary
Sherif Mogawer
Samah Abd Elhamid
Sahr Alwakil
Fatma Aboelkasem
Hatem El Sabaawy
Olfat Abdelhalim
Publication date: 01-01-2013
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 1/2013
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-012-1298-8

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Results

Conclusion

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