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Journal of Cancer Research and Clinical Oncology

Published in:

Open Access 01-01-2012 | Original Paper

A cluster of specified microRNAs in peripheral blood as biomarkers for metastatic non-small-cell lung cancer by stem-loop RT-PCR

Authors: Qingfeng Lin, Weidong Mao, Yongqian Shu, Feng Lin, Shaoping Liu, Hua Shen, Wen Gao, Suqing Li, Dong Shen

Published in: Journal of Cancer Research and Clinical Oncology | Issue 1/2012

Abstract

Purpose

To investigate the levels for some specified microRNAs in human’s peripheral blood so as to determine whether they can serve as biomarkers for metastatic non-small-cell lung cancer.

Methods

Use a quantitative stem-loop RT-PCR method to examine the serum levels for certain microRNAs including has-miR-125a-5p, has-miR-126, has-miR-183, has-miR-200, has-miR-221, and has-miR-222 from the patients with Stage IV, Stage I/II non-small-cell lung cancer and the controls.

Results

There was statistical difference in the serum levels for hsa-miR-126, hsa-miR-183, and hsa-miR-222 between the controls and the Stage IV patients, but not for has-miR-125a-5p, has-miR-200 and has-miR-221. It also showed statistical difference for hsa-miR-126 and hsa-miR-183 between the Stage I/II patients and Stage IV patients, but not between the controls and Stage I/II patients.

Conclusion

Hsa-miR-126 and hsa-miR-183 may serve as potential serum biomarkers for metastatic non-small-cell lung cancer.

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Title: A cluster of specified microRNAs in peripheral blood as biomarkers for metastatic non-small-cell lung cancer by stem-loop RT-PCR
Authors: Qingfeng Lin
Weidong Mao
Yongqian Shu
Feng Lin
Shaoping Liu
Hua Shen
Wen Gao
Suqing Li
Dong Shen
Publication date: 01-01-2012
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 1/2012
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-011-1068-z

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A cluster of specified microRNAs in peripheral blood as biomarkers for metastatic non-small-cell lung cancer by stem-loop RT-PCR

Abstract

Purpose

Methods

Results

Conclusion

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