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Journal of Cancer Research and Clinical Oncology

Published in:

01-12-2009 | Original Paper

Gambogic acid reduced bcl-2 expression via p53 in human breast MCF-7 cancer cells

Authors: Hongyan Gu, Shuyun Rao, Jie Zhao, Jia Wang, Rong Mu, Jingjing Rong, Lei Tao, Qi Qi, Qidong You, Qinglong Guo

Published in: Journal of Cancer Research and Clinical Oncology | Issue 12/2009

Abstract

Purpose

In this study, we investigated the correlation between p53 and bcl-2 in gambogic acid (GA)-induced apoptosis.

Method

MTT assay was employed to evaluate MCF-7 cell viability after GA treatment. Cell morphological changes were observed follow-up under the inverted microscope after GA withdrawal. To observe the cell apoptosis, DAPI staining was used. Meanwhile, p53 small interfering RNA (si-RNA) was adopted to knock p53 down. All expressions of p53 and bcl-2 were evaluated by Western blot analysis.

Results

MTT assay showed that GA inhibited MCF-7 cell growth effectively in a time-dependent manner. With 0.5 h GA treatment, p53 was significantly increased, whereas bcl-2 was reduced potently with 6 h GA treatment. After GA withdrawal, p53 expressions were maintained in high levels. Furthermore, bcl-2 decreasing was attenuated after co-treatment with PFT alpha, a p53 transcription blocker. Same result was observed after p53 knock-down by p53 si-RNA.

Conclusions

Gambogic acid induced human breast cancer cells MCF-7 apoptosis by reducing bcl-2 expression via p53.

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Title: Gambogic acid reduced bcl-2 expression via p53 in human breast MCF-7 cancer cells
Authors: Hongyan Gu
Shuyun Rao
Jie Zhao
Jia Wang
Rong Mu
Jingjing Rong
Lei Tao
Qi Qi
Qidong You
Qinglong Guo
Publication date: 01-12-2009
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 12/2009
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-009-0624-2

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Gambogic acid reduced bcl-2 expression via p53 in human breast MCF-7 cancer cells

Abstract

Purpose

Method

Results

Conclusions

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Abstract

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