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Journal of Cancer Research and Clinical Oncology

Published in:

01-06-2007 | Original Paper

Effect of lycopene on insulin-like growth factor-I, IGF binding protein-3 and IGF type-I receptor in prostate cancer cells

Authors: P. Kanagaraj, M. R. Vijayababu, B. Ravisankar, J. Anbalagan, M. M. Aruldhas, J. Arunakaran

Published in: Journal of Cancer Research and Clinical Oncology | Issue 6/2007

Abstract

Purpose

Prostate cancer is the second most common cancer that leads to death in elderly men. The risk of prostate cancer prevalence is often associated with the elevated level of insulin-like growth factor-I (IGF-I) and decreased level of IGF-binding protein 3 (IGFBP-3). Lycopene, a carotenoid, reduces the proliferation of cancer cells and induces apoptosis. Hence, higher intake of lycopene can be associated with the lower risk of prostate cancer. However, the mechanism of action of lycopene in the prevention of prostate cancer is still unclear. The present study was carried out to study the effects of lycopene on the components of IGF system and apoptosis in androgen-independent prostate cancer cells (PC-3 cells).

Methods

PC-3 cells were treated with various concentrations of lycopene, (20, 40 and 60 μM) for 24, 48, 72 and 96 h. IGF-I, IGFBP-3 and IGF-I receptor (IGF-IR) levels in lycopene-treated cells were evaluated. Annexin V and propidium iodide (PI) binding studies were done to assess apoptosis.

Results

PC-3 cells treated with lycopene showed a significant decrease in cell proliferation. Lycopene, at a dose of 40 μM, significantly increased the level of IGFBP-3. Lycopene-induced apoptosis was confirmed by annexin V and PI binding. Lycopene-induced DNA fragmentation was absent after 24 h treatment whereas the same was observed after 48 h treatment. There was a significant decrease in the IGF-IR expression after the cells were treated with lycopene and IGF-I.

Conclusion

The data obtained suggest that the components of the IGF system may act as a positive regulator of lycopene-induced apoptosis in PC-3 cells. Thus, the observed lycopene-induced biological effects and their associated mechanisms are encouraging and may lead to the development of a highly successful drug for the treatment of prostate cancer.

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Title: Effect of lycopene on insulin-like growth factor-I, IGF binding protein-3 and IGF type-I receptor in prostate cancer cells
Authors: P. Kanagaraj
M. R. Vijayababu
B. Ravisankar
J. Anbalagan
M. M. Aruldhas
J. Arunakaran
Publication date: 01-06-2007
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 6/2007
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-006-0177-6

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Effect of lycopene on insulin-like growth factor-I, IGF binding protein-3 and IGF type-I receptor in prostate cancer cells

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

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