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Journal of Cancer Research and Clinical Oncology

Published in:

01-01-2005 | Original Paper

A single nucleotide polymorphism in the MMP-9 promoter affects tumor progression and invasive phenotype of gastric cancer

Authors: Shunji Matsumura, Naohide Oue, Hirofumi Nakayama, Yasuhiko Kitadai, Kazuhiro Yoshida, Yoshiyuki Yamaguchi, Kazue Imai, Kei Nakachi, Keisuke Matsusaki, Kazuaki Chayama, Wataru Yasui

Published in: Journal of Cancer Research and Clinical Oncology | Issue 1/2005

Abstract

Purpose

Matrix metalloproteinase-9 (MMP-9, gelatinase B) plays a key role in cancer invasion and metastasis by degradating the extracellular matrix (ECM) and basement membrane barriers. A cytosine (C)-thymidine (T) single nucleotide polymorphism (SNP) at position –1562 in the MMP-9 promoter is reported to affect expression of this gene. The purpose of this study was to investigate the relation between the –1562 C/T polymorphism and the development and progression of gastric cancer.

Methods

The study population included 177 gastric cancer patients and 224 healthy control subjects. The SNP in the MMP-9 promoter was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing. Genotype frequencies were compared between patients and controls, and the association of genotypes with clinicopathological features was studied.

Results

Genotype frequencies in gastric cancer patients were similar to those in control subjects (P = 0.223). However, significant association was found between degree of tumor invasion, clinical stage, and lymphatic invasion and the MMP-9 polymorphism in gastric cancer patients (P<0.05, for each).

Conclusions

Our results indicate that the T allele in the MMP-9 promoter is associated with the invasive phenotype of gastric cancer.

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Title: A single nucleotide polymorphism in the MMP-9 promoter affects tumor progression and invasive phenotype of gastric cancer
Authors: Shunji Matsumura
Naohide Oue
Hirofumi Nakayama
Yasuhiko Kitadai
Kazuhiro Yoshida
Yoshiyuki Yamaguchi
Kazue Imai
Kei Nakachi
Keisuke Matsusaki
Kazuaki Chayama
Wataru Yasui
Publication date: 01-01-2005
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 1/2005
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-004-0621-4

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A single nucleotide polymorphism in the MMP-9 promoter affects tumor progression and invasive phenotype of gastric cancer

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Purpose

Methods

Results

Conclusions

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