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Journal of Cancer Research and Clinical Oncology

Published in:

01-06-2003 | Original Paper

Analysis of DLC-1 expression in human breast cancer

Authors: Marlies Plaumann, Susanne Seitz, Renate Frege, Lope Estevez-Schwarz, Siegfried Scherneck

Published in: Journal of Cancer Research and Clinical Oncology | Issue 6/2003

Abstract

The chromosome region 8p12-p22 shows frequent allelic loss in many neoplasms, including breast cancer (BC). The DLC-1 gene, located on 8p21-p22, might be a candidate tumor suppressor gene in this region. To evaluate the involvement of DLC-1 in breast carcinogenesis we studied DLC-1 mRNA expression in a panel of 14 primary human BC and the corresponding normal breast cells as well as 8 BC cell lines. Low levels or absence of DLC-1 mRNA were observed in 57% of primary BC and 62.5% of BC cell lines, respectively. We could not find any correlation between DLC-1mRNA expression and deletions at the DLC-1 locus. Transfection of the gene into DLC-1 deficient T-47D cells raised the DLC-1 mRNA level and resulted in inhibition of cell growth and reduced colony-forming capacity. Our results indicate a role of DLC-1 in BC carcinogenesis.

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Title: Analysis of DLC-1 expression in human breast cancer
Authors: Marlies Plaumann
Susanne Seitz
Renate Frege
Lope Estevez-Schwarz
Siegfried Scherneck
Publication date: 01-06-2003
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 6/2003
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-003-0440-z

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