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European Journal of Pediatrics
Published in: European Journal of Pediatrics 11/2007

01-11-2007 | Short Report

Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis

Authors: Alida M. van Kruijssen, Kate E. Templeton, Roos N. van der Plas, H. Rogier van Doorn, Eric C. J. Claas, Ram N. Sukhai, Ed J. Kuijper

Published in: European Journal of Pediatrics | Issue 11/2007

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Abstract

The use of a multiplex respiratory real-time PCR in patients clinically suspected of pertussis increases the number of pathogens detected.
Literature
1.
Cherry JD, Grimprel E, Guiso N, Heininger U, Mertsola J (2005) Defining pertussis epidemiology: clinical, microbiologic and serologic perspectives. Pediatr Infect Dis J 24(suppl):S25–S34
2.
Jackson LA, Cherry JD, Wang SP, Grayston JT (2000) Frequency of serological evidence of Bordetella infections and mixed infections with other respiratory pathogens in university students with cough illnesses. Clin Infect Dis 31:3–6PubMedCrossRef
3.
Loeffelholz MJ, Thompson CJ, Long KS, Gilchrist MJ (1999) Comparison of PCR, culture, and direct fluorescent-antibody testing for detection of Bordetella pertussis. J Clin Microbiol 37:2872–2876PubMed
4.
Templeton KE, Scheltinga SA, van den Eden WCJFM, Graffelman AW, van den Broek PJ, Claas EC (2005) Improved diagnosis of etiology of community-acquired pneumonia using real-time PCR. Clin Infect Dis 41:345–351PubMedCrossRef
5.
Templeton KE, Scheltinga SA, van der Zee, Diederen BM, van Kuijssen AM, Goossens H, Kuijper EJ, Claas EC (2003) Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis. J Clin Microbiol 41:4121–4126PubMedCrossRef
6.
Metadata
Title
Detection of respiratory pathogens by real-time PCR in children with clinical suspicion of pertussis
Authors
Alida M. van Kruijssen
Kate E. Templeton
Roos N. van der Plas
H. Rogier van Doorn
Eric C. J. Claas
Ram N. Sukhai
Ed J. Kuijper
Publication date
01-11-2007
Publisher
Springer-Verlag
Published in
European Journal of Pediatrics / Issue 11/2007
Print ISSN: 0340-6199
Electronic ISSN: 1432-1076
DOI
https://doi.org/10.1007/s00431-006-0378-7

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