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Published in: Medical Microbiology and Immunology 3/2014

01-06-2014 | Original Investigation

Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice

Authors: Romina Jimena Fernandez-Brando, Gabriel Cabrera, Ariela Baschkier, María Pilar Mejías, Cecilia Analia Panek, Elizabeth Miliwebsky, María Jimena Abrey-Recalde, Leticia Verónica Bentancor, María Victoria Ramos, Marta Rivas, Marina Sandra Palermo

Published in: Medical Microbiology and Immunology | Issue 3/2014

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Abstract

Hemolytic uremic syndrome (HUS) is the major complication of gastrointestinal infections with enterohemorrhagic Escherichia coli (EHEC) and is mediated by the production of Shiga toxins (Stx). Although it has been previously reported that not only HUS patients but healthy children have anti-Stx antibodies, very little is known about how these infections impact on mucosal immune system to generate a specific immune response. This work aimed to evaluate the immune responses elicited after a single oral dose of EHEC in a mouse model of HUS at weaning. We found sequential activation of T and B lymphocytes together with an increased percentage of IgA-bearing B cells in Peyer’s patches and mesenteric lymph nodes. We also found fecal anti-EHEC IgA and serum anti-Stx2 IgG in EHEC-inoculated mice. Besides, these mice were partially protected against an intravenous challenge with Stx2. These data demonstrate that one episode of EHEC infection is enough to induce activation in the gut-associated lymphoid tissue, especially the B cell compartment, and lead to the production of specific IgA in mucosal tissue and the generation of systemic protection against Stx2 in a percentage of intragastrically inoculated mice. These data also support the epidemiologic observation that a second episode of HUS is very rare.
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Metadata
Title
Oral administration of Shiga toxin-producing Escherichia coli induces intestinal and systemic specific immune response in mice
Authors
Romina Jimena Fernandez-Brando
Gabriel Cabrera
Ariela Baschkier
María Pilar Mejías
Cecilia Analia Panek
Elizabeth Miliwebsky
María Jimena Abrey-Recalde
Leticia Verónica Bentancor
María Victoria Ramos
Marta Rivas
Marina Sandra Palermo
Publication date
01-06-2014
Publisher
Springer Berlin Heidelberg
Published in
Medical Microbiology and Immunology / Issue 3/2014
Print ISSN: 0300-8584
Electronic ISSN: 1432-1831
DOI
https://doi.org/10.1007/s00430-013-0325-y

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