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01-02-2011 | Rapid Communication

Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells

Authors: Junji Xing, Shuai Wang, You Li, Hong Guo, Lei Zhao, Weiwei Pan, Fusen Lin, Huifang Zhu, Lei Wang, Meili Li, Lin Wang, Chunfu Zheng

Published in: Medical Microbiology and Immunology | Issue 1/2011

Abstract

In this study, we presented the construction of a library of expression clones for the herpes simplex virus type 1 (HSV-1) proteome and subcellular localization map of HSV-1 proteins in living cells using yellow fluorescent protein (YFP) fusion proteins. As a result, 21 proteins showed cytoplasmic or subcytoplasmic localization, 16 proteins showed nuclear or subnuclear localization, and others were present both in the nucleus and cytoplasm. Interestingly, most capsid proteins showed enriched or exclusive localization in the nucleus, and most of the envelope proteins showed cytoplasmic localization, suggesting that subcellular localization of the proteins correlated with their functions during virus replication. These results present a subcellular localization map of HSV-1 proteins in living cells, which provide useful information to further characterize the functions of these proteins.

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Title: Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells
Authors: Junji Xing
Shuai Wang
You Li
Hong Guo
Lei Zhao
Weiwei Pan
Fusen Lin
Huifang Zhu
Lei Wang
Meili Li
Lin Wang
Chunfu Zheng
Publication date: 01-02-2011
Publisher: Springer-Verlag
Published in: Medical Microbiology and Immunology / Issue 1/2011
Print ISSN: 0300-8584
Electronic ISSN: 1432-1831
DOI: https://doi.org/10.1007/s00430-010-0175-9

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