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01-11-2004 | Original Investigation

Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus

Authors: Andreas Lucht, Roland Grunow, Christian Otterbein, Peggy Möller, Heinz Feldmann, Stephan Becker

Published in: Medical Microbiology and Immunology | Issue 4/2004

Abstract

Ebola virus (EBOV) causes severe outbreaks of Ebola hemorrhagic fever in endemic regions of Africa and is considered to be of impact for other parts of the world as an imported viral disease. To develop a new diagnostic test, monoclonal antibodies to EBOV were produced from mice immunized with inactivated EBOV species Zaire. Antibodies directed against the viral glycoprotein GP were characterized by ELISA, Western blot and immunofluorescence analyses. An antigen capture ELISA was established, which is specific for EBOV-Zaire and shows a sensitivity of approximately 10³ plaque-forming units/ml. Since the ELISA is able to detect even SDS-inactivated EBOV in spiked human sera, it could complement the existing diagnostic tools in the field and in routine laboratories where high containment facilities are not available.

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Title: Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus
Authors: Andreas Lucht
Roland Grunow
Christian Otterbein
Peggy Möller
Heinz Feldmann
Stephan Becker
Publication date: 01-11-2004
Publisher: Springer-Verlag
Published in: Medical Microbiology and Immunology / Issue 4/2004
Print ISSN: 0300-8584
Electronic ISSN: 1432-1831
DOI: https://doi.org/10.1007/s00430-003-0204-z

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