Top

Published in:

01-05-2014 | Original Article

Projections from the lateral vestibular nucleus to the spinal cord in the mouse

Authors: Huazheng Liang, Timea Bácskai, Charles Watson, George Paxinos

Published in: Brain Structure and Function | Issue 3/2014

Abstract

The present study investigated the projections from the lateral vestibular nucleus (LVe) to the spinal cord using retrograde and anterograde tracers. Retrogradely labeled neurons were found after fluoro-gold injections into both the cervical and lumbar cord, with a smaller number of labeled neurons seen after lumbar cord injections. Labeled neurons in the LVe were found in clusters at caudal levels of the nucleus, and a small gap separated these clusters from labeled neurons in the spinal vestibular nucleus (SpVe). In the anterograde study, BDA-labeled fiber tracts were found in both the ventral and ventrolateral funiculi on the ipsilateral side. These fibers terminated in laminae 6–9. Some fibers were continuous with boutons in contact with motor neurons in both the medial and lateral motor neuron columns. In the lumbar and sacral segments, some collaterals from the ipsilateral vestibulospinal tracts were found on the contralateral side, and these fibers mainly terminated in laminae 6–8. The present study reveals for the first time the fiber terminations of the lateral vestibular nucleus in the mouse spinal cord and therefore enhances future functional studies of the vestibulospinal system.

Abercrombie M (1946) Estimation of nuclear population from microtome sections. Anat Rec 94:239–247PubMedCrossRef

Abzug C, Maeda M, Peterson BW, Wilson VJ (1974) Cervical branching of lumbar vestibulospinal axons. J Physiol 243:499–522PubMedCentralPubMed

Auclair F, Belanger MC, Marchand R (1993) Ontogenetic study of early brain stem projections to the spinal cord in the rat. Brain Res Bull 30:281–289PubMedCrossRef

Bácskai T, Székely G, Matesz C (2002) Ascending and descending projections of the lateral vestibular nucleus in the rat. Acta Biol Hung 53:7–21PubMedCrossRef

Boyle R (2000) Morphology of lumbar-projecting lateral vestibulospinal neurons in the brainstem and cervical spinal cord in the squirrel monkey. Arch Ital Biol 138:107–122PubMed

Bracci E, Ballerini L, Nistri A (1996) Localization of rhythmogenic networks responsible for spontaneous bursts induced by strychnine and bicuculline in the rat isolated spinal cord. J Neurosci 16:7063–7076PubMed

Brink EE, Hirai N, Wilson VJ (1980) Influence of neck afferents on vestibulospinal neurons. Exp Brain Res 38:285–292PubMedCrossRef

Cina C, Hochman S (2000) Diffuse distribution of sulforhodamine labeled neurons during serotonin-evoked locomotion in the neonatal rat thoracolumbar spinal cord. J Comp Neurol 423:590–602PubMedCrossRef

Dai X, Noga BR, Douglas JR, Jordan LM (2005) Localization of spinal neurons activated during locomotion using the c-fos immunohistochemical method. J Neurophysiol 93:3442–3452PubMedCrossRef

de Boer-van Huizen RT, ten Donkelaar HJ (1999) Early development of descending supraspinal pathways: a tracing study in fixed and isolated rat embryos. Anat Embryol 199:539–547PubMedCrossRef

Du Beau A, Shakya Shrestha S, Bannatyne BA, Jalicy SM, Linnen S, Maxwell DJ (2012) Neurotransmitter phenotypes of descending systems in the rat lumbar spinal cord. Neuroscience 227:67–79PubMedCrossRef

Franklin KBJ, Paxinos G (2008) The mouse brain in stereotaxic coordinates, 3rd edn. Elsevier Academic Press, San Diego

Grillner S, Hongo T, Lund S (1970) The vestibulospinal tract. Effects on alpha-motoneurones in the lumbosacral spinal cord in the cat. Exp Brain Res 10:94–120PubMedCrossRef

Hayes NL, Rustioni A (1981) Descending projections from brainstem and sensorimotor cortex to spinal enlargements in the cat. Single and double retrograde tracer studies. Exp Brain Res 41:89–107PubMedCrossRef

Huisman AM, Ververs B, Cavada C, Kuypers HG (1984) Collateralization of brainstem pathways in the spinal ventral horn in rat as demonstrated with the retrograde fluorescent double-labeling technique. Brain Res 300:362–367PubMedCrossRef

Isu N, Uchino Y, Nakashima H, Satoh S, Ichikawa T, Watanabe S (1988) Axonal trajectories of posterior canal-activated secondary vestibular neurons and their coactivation of extraocular and neck flexor motoneurons in the cat. Exp Brain Res 70:181–191PubMed

Kasumacic N, Glover JC, Perreault MC (2010) Segmental patterns of vestibular-mediated synaptic inputs to axial and limb motoneurons in the neonatal mouse assessed by optical recording. J Physiol 588:4905–4925PubMedCentralPubMedCrossRef

Kjaerulff O, Barajon I, Kiehn O (1994) Sulphorhodamine-labeled cells in the neonatal rat spinal cord following chemically induced locomotor activity in vitro. J Physiol 478:265–273PubMedCentralPubMed

Kudo N, Furukawa F, Okado N (1993) Development of descending fibers to the rat embryonic spinalcord. Neurosci Res 16:131–141PubMedCrossRef

Kuze B, Matsuyama K, Matsui T, Miyata H, Mori S (1999) Segment-specific branching patterns of single vestibulospinal tract axons arising from the lateral vestibular nucleus in the cat: a PHA-L tracing study. J Comp Neurol. 414:80–96PubMedCrossRef

Leong SK, Shieh JY, Wong WC (1984) Localizing spinal-cord—projecting neurons in adult albino rats. J Comp Neurol 228:1–17PubMedCrossRef

Liang H, Paxinos G, Watson C (2011) Projections from the brain to the spinal cord in the mouse. Brain Struct Funct 215:159–186PubMedCrossRef

Masson RL, Sparkes ML, Ritz LA (1991) Descending projections to the rat sacrocaudal spinal cord. J Comp Neurol 302:120–130CrossRef

Matesz C, Bácskai T, Nagy E, Halasi G, Kulik A (2002) Efferent connections of the vestibular nuclei in the rat: a neuromorphological study using PHA-L. Brain Res Bull 57:313–315PubMedCrossRef

Paxinos G, Franklin KBJ (2013) The Mouse brain in stereotaxic coordinates, 4th edn. Elsevier Academic Press, San Diego

Paxinos G, Watson C (1982) The Rat brain in stereotaxic coordinates. Academic Press, Sydney

Pompeiano O (1972) Vestibulospinal relations: vestibular influences on gamma motoneurons and primary afferents. Prog Brain Res 37:263–296PubMedCrossRef

Rice CD, Weber SA, Waggoner AL, Jessell ME, Yates BJ (2010) Mapping of neural pathways that influence diaphragm activity and project to the lumbar spinal cord in cats. Exp Brain Res 203:205–211PubMedCentralPubMedCrossRef

Rose PK, Wainwright K, Neuber-Hess M (1992) Connections from the lateral vestibular nucleus to the upper cervical spinal cord of the cat: a study with the anterograde tracer PHA-L. J Comp Neurol 321:312–324PubMedCrossRef

Rose PK, Ely S, Norkum V, Neuber-Hess M (1999) Projections from the lateral vestibular nucleus to the upper cervical spinal cord of the cat: a correlative light and electron microscopic study of axon terminals stained with PHA-L. J Comp Neurol 410:571–585PubMedCrossRef

Shen P, Arnold AP, Micevych PE (1990) Supraspinal projections to the ventromedial lumbar spinal cord in adult male rats. J Comp Neurol 300:263–272PubMedCrossRef

Shinoda Y, Ohgaki T, Sugiuchi Y, Futami T (1989) Comparison of the branching patterns of lateral and medial vestibulospinal tract axons in the cervical spinal cord. Prog Brain Res 80:137–147PubMedCrossRef

Tresch MC, Kiehn O (1999) Coding of locomotor phase in populations of neurons in rostral and caudal segments of the neonatal rat lumbar spinal cord. J Neurophysiol 82:3563–3574PubMed

Valla J, Delfini C, Diagne M, Pinganaud G, Buisseret P, Buisseret-Delmas C (2003) Vestibulotrigeminal and vestibulospinal projections in rats: retrograde tracing coupled to glutamic acid decarboxylase immunoreactivity. Neurosci Lett 340:225–228PubMedCrossRef

VanderHorst VG, Ulfhake B (2006) The organization of the brainstem and spinal cord of the mouse: relationships between monoaminergic, cholinergic, and spinal projection systems. J Chem Neuroanat 31:2–36PubMedCrossRef

Wada N, Sugita S, Jouzaki A, Tokuriki M (1993) Descending projections to coccygeal spinal segments in the cat. J Anat 182:259–265PubMedCentralPubMed

Watson C (2012) Hindbrain. In: Watson C, Paxinos G, Puelles L (eds) The Mouse Nervous System, chap. 12. Elsevier Academic Press, San Diego, pp 408–409

Watson C, Paxinos G (2010) Chemoarchitectonic Atlas of the Mouse Brain. Elsevier Academic Press, San Diego

Watson C, Paxinos G, Kayalioglu G, Heise C (2009) Atlas of the mouse spinal cord. In: Watson C, Paxinos G, Kayalioglu G (eds) The Spinal cord. Elsevier Academic Press, San Diego, pp 308–379CrossRef

Wilson VJ, Yoshida M (1969) Comparison of effects of stimulation of Deiters’ nucleus and medial longitudinal fasciculus on neck, forelimb, and hindlimb motoneurons. J Neurophysiol 32:743–758PubMed

Wilson VJ, Wylie RM, Marco LA (1967) Projection to the spinal cord from the medial and descending vestibular nuclei of the cat. Nature 215:429–430PubMedCrossRef

Wilson VJ, Yoshida M, Schor RH (1970) Supraspinal monosynaptic excitation and inhibition of thoracic back montoneurons. Exp Brain Res 11:282–295PubMedCrossRef

Zemlan FP, Kow LM, Morrell JI, Pfaff DW (1979) Descending tracts of the lateral columns of the rat spinal cord: a study using the horseradish peroxidase and silver impregnation techniques. J Anat 128:489–512PubMedCentralPubMed

Title: Projections from the lateral vestibular nucleus to the spinal cord in the mouse
Authors: Huazheng Liang
Timea Bácskai
Charles Watson
George Paxinos
Publication date: 01-05-2014
Publisher: Springer Berlin Heidelberg
Published in: Brain Structure and Function / Issue 3/2014
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-013-0536-4

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Projections from the lateral vestibular nucleus to the spinal cord in the mouse

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2014

Neurogenesis, gliogenesis and the developing chicken optic tectum: an immunohistochemical and ultrastructural analysis

Probing emotional influences on cognitive control: an ALE meta-analysis of cognition emotion interactions

Interhemispheric and intrahemispheric connectivity and manual skills in children with unilateral cerebral palsy

Hyperacusis-associated pathological resting-state brain oscillations in the tinnitus brain: a hyperresponsiveness network with paradoxically inactive auditory cortex

Amygdalar connections of the macaque areas 45A and 45B

The eye of the self: precuneus volume and visual perspective during autobiographical memory retrieval