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Published in: Brain Structure and Function 4/2005

01-11-2005 | Original Article

Intermediate filament immunohistochemistry of astroglial cells in the leopard gecko, Eublepharis macularius

Authors: Maurizio Lazzari, Valeria Franceschini

Published in: Brain Structure and Function | Issue 4/2005

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Abstract

The distribution of intermediate filament molecular markers, glial fibrillary acidic protein (GFAP) and vimentin, has been studied in the central nervous system (CNS) of the adult leopard gecko, Eublepharis macularius. This immunohistochemical study points out the presence of different astroglial cell types. The main pattern is constituted by ependymal radial glia, which have their cell bodies located in the ependymal layer throughout the brain ventricular system. Radial glia proper or radial astrocytes show their cell bodies displaced from the ependymal layer into a periependymal zone and are observed only in the spinal cord. Star-shaped astrocytes are scarce. They are detected in the ventral and lateral regions of the diencephalon and mesencephalon, in the superficial layer of the optic tectum, in the ventral medulla oblongata, and in the ventral and lateral spinal cord. In the different regions of the CNS, the staining intensity appears not to be identical even in the same cellular type. The results reported in the present study show an heterogeneous feature of the astroglial pattern in E. macularius.
Literature
go back to reference Adams JC (1981) Heavy-metal intensification of DAB-based reaction product. J Histochem Cytochem 29:775PubMed Adams JC (1981) Heavy-metal intensification of DAB-based reaction product. J Histochem Cytochem 29:775PubMed
go back to reference Alvarez-Buylla A, Buskirk DR, Nottebohm F (1987) Monoclonal antibody reveals radial glia in adult avian brain. J Comp Neurol 264:159–170PubMedCrossRef Alvarez-Buylla A, Buskirk DR, Nottebohm F (1987) Monoclonal antibody reveals radial glia in adult avian brain. J Comp Neurol 264:159–170PubMedCrossRef
go back to reference Bennett GS, Fellini SA, Holtzer H (1978) Immunofluorescent visualization of 100 A filaments in different cultured chick embryo cell types. Differentiation 12:71–82PubMedCrossRef Bennett GS, Fellini SA, Holtzer H (1978) Immunofluorescent visualization of 100 A filaments in different cultured chick embryo cell types. Differentiation 12:71–82PubMedCrossRef
go back to reference Bodega G, Suarez I, Rubio M, Fernandez B (1990) Distribution and characteristics of the different astroglial cell types in the adult lizard (Lacerta lepida) spinal cord. Anat Embryol 181:567–575PubMedCrossRef Bodega G, Suarez I, Rubio M, Fernandez B (1990) Distribution and characteristics of the different astroglial cell types in the adult lizard (Lacerta lepida) spinal cord. Anat Embryol 181:567–575PubMedCrossRef
go back to reference Bodega G, Suarez I, Rubio M, Villaba RM, Fernandez B (1993) Astroglial pattern in the spinal cord of the adult barbel (Barbus comiza). Anat Embryol 187:385–395PubMedCrossRef Bodega G, Suarez I, Rubio M, Villaba RM, Fernandez B (1993) Astroglial pattern in the spinal cord of the adult barbel (Barbus comiza). Anat Embryol 187:385–395PubMedCrossRef
go back to reference Bodega G, Suárez I, Rubio M, Fernández B (1994) Ependyma: phylogenetic evolution of glial fibrillary acidic protein (GFAP) and vimentin expression in vertebrate spinal cord. Histochemistry 102:113–122PubMedCrossRef Bodega G, Suárez I, Rubio M, Fernández B (1994) Ependyma: phylogenetic evolution of glial fibrillary acidic protein (GFAP) and vimentin expression in vertebrate spinal cord. Histochemistry 102:113–122PubMedCrossRef
go back to reference Cardone B, Roots BJ (1990) Comparative immunohistochemical study of glial filament proteins (glial fibrillary acidic protein and vimentin) in goldfish, octopus and snail. Glia 3:180–192PubMedCrossRef Cardone B, Roots BJ (1990) Comparative immunohistochemical study of glial filament proteins (glial fibrillary acidic protein and vimentin) in goldfish, octopus and snail. Glia 3:180–192PubMedCrossRef
go back to reference Chouaf L, Didier-Bazes M, Aguera M, Tardy M, Sallanon M, Kitahama K, Belin MF (1989) Comparative marker analysis of the ependymocytes in the subcommissural organ in four different mammalian species. Cell Tissue Res 257:255–262PubMedCrossRef Chouaf L, Didier-Bazes M, Aguera M, Tardy M, Sallanon M, Kitahama K, Belin MF (1989) Comparative marker analysis of the ependymocytes in the subcommissural organ in four different mammalian species. Cell Tissue Res 257:255–262PubMedCrossRef
go back to reference Dahl D, Bignami A (1973) Immunochemical and immunofluorescence studies of the GFAP in vertebrates. Brain Res 61:279–293PubMedCrossRef Dahl D, Bignami A (1973) Immunochemical and immunofluorescence studies of the GFAP in vertebrates. Brain Res 61:279–293PubMedCrossRef
go back to reference Dahl D, Bignami A (1985) Intermediate filaments in nervous tissue. In: Shay JW (ed) Cell and muscle motility, vol 6. Plenum Press New York, pp 75–96 Dahl D, Bignami A (1985) Intermediate filaments in nervous tissue. In: Shay JW (ed) Cell and muscle motility, vol 6. Plenum Press New York, pp 75–96
go back to reference Dahl D, Crosby CJ, Sethi JS, Bignami A (1985) Glial fibrillary acidic (GFA) protein in vertebrates: immunofluorescence and immunoblotting study with monoclonal and polyclonal antibodies. J Comp Neurol 239:75–88PubMedCrossRef Dahl D, Crosby CJ, Sethi JS, Bignami A (1985) Glial fibrillary acidic (GFA) protein in vertebrates: immunofluorescence and immunoblotting study with monoclonal and polyclonal antibodies. J Comp Neurol 239:75–88PubMedCrossRef
go back to reference Ebner FF, Colonnier M (1975) Synaptic patterns in the visual cortex of turtle: an electron microscopic study. J Comp Neurol 160:51–80PubMedCrossRef Ebner FF, Colonnier M (1975) Synaptic patterns in the visual cortex of turtle: an electron microscopic study. J Comp Neurol 160:51–80PubMedCrossRef
go back to reference Elmquist JK, Swanson JJ, Sakaguchi DS, Ross LR, Jacobson CD (1994) Developmental distribution of GFAP and vimentin in the Brazilian opossum brain. J Comp Neurol 344:283–296PubMedCrossRef Elmquist JK, Swanson JJ, Sakaguchi DS, Ross LR, Jacobson CD (1994) Developmental distribution of GFAP and vimentin in the Brazilian opossum brain. J Comp Neurol 344:283–296PubMedCrossRef
go back to reference Kalman M (1998) Astroglial architecture of the carp (Cyprinus carpio) brain as revealed by immunocytochemical staining against glial fibrillary acidic protein (GFAP). Anat Embryol 198:409–433PubMedCrossRef Kalman M (1998) Astroglial architecture of the carp (Cyprinus carpio) brain as revealed by immunocytochemical staining against glial fibrillary acidic protein (GFAP). Anat Embryol 198:409–433PubMedCrossRef
go back to reference Kalman M, Pritz MB (2001) Glial fibrillary acidic protein-immunopositive structures in the brain of a crocodilian, Caiman crocodilus, and its bearing on the evolution of astroglia. J Comp Neurol 431:460–480PubMedCrossRef Kalman M, Pritz MB (2001) Glial fibrillary acidic protein-immunopositive structures in the brain of a crocodilian, Caiman crocodilus, and its bearing on the evolution of astroglia. J Comp Neurol 431:460–480PubMedCrossRef
go back to reference Kalman M, Szekely A, Csillag A (1993) Distribution of glial fibrillary acidic protein-immunopositive structures in the brain of the domestic chicken (Gallus domesticus). J Comp Neurol 330:221–237PubMedCrossRef Kalman M, Szekely A, Csillag A (1993) Distribution of glial fibrillary acidic protein-immunopositive structures in the brain of the domestic chicken (Gallus domesticus). J Comp Neurol 330:221–237PubMedCrossRef
go back to reference Kalman M, Kiss A, Majorossy K (1994) Distribution of glial fibrillary acidic protein-immunopositive structures in the brain of the red-eared freshwater turtle (Pseudemys scripta elegans). Anat Embryol 189:421–434PubMedCrossRef Kalman M, Kiss A, Majorossy K (1994) Distribution of glial fibrillary acidic protein-immunopositive structures in the brain of the red-eared freshwater turtle (Pseudemys scripta elegans). Anat Embryol 189:421–434PubMedCrossRef
go back to reference Kalman M, Martin-Partido G, Hidalgo-Sanchez M, Majorossy K (1997) Distribution of glial fibrillary acidic protein–immunopositive structures in the developing brain of the turtle Mauremys leprosa. Anat Embryol 196:47–65PubMedCrossRef Kalman M, Martin-Partido G, Hidalgo-Sanchez M, Majorossy K (1997) Distribution of glial fibrillary acidic protein–immunopositive structures in the developing brain of the turtle Mauremys leprosa. Anat Embryol 196:47–65PubMedCrossRef
go back to reference Kalman M, Szekely AD, Csillag A (1998) Distribution of glial fibrillary acidic protein and vimentin-immunopositive elements in the developing chicken brain from hatch to adulthood. Anat Embryol 198:213–235PubMedCrossRef Kalman M, Szekely AD, Csillag A (1998) Distribution of glial fibrillary acidic protein and vimentin-immunopositive elements in the developing chicken brain from hatch to adulthood. Anat Embryol 198:213–235PubMedCrossRef
go back to reference Lauro GM, Fonti R, Margotta V (1991) Phylogenetic evolution of intermediate filament associated proteins in ependymal cells of several adult poikilotherm vertebrates. J Hirnforsch 32:257–261PubMed Lauro GM, Fonti R, Margotta V (1991) Phylogenetic evolution of intermediate filament associated proteins in ependymal cells of several adult poikilotherm vertebrates. J Hirnforsch 32:257–261PubMed
go back to reference Lazzari M, Franceschini V (2001) Glial fibrillary acidic protein and vimentin immunoreactivity of astroglial cells in the central nervous system of adult Podarcis sicula (Squamata, Lacertidae). J Anat 198:67–75PubMedCrossRef Lazzari M, Franceschini V (2001) Glial fibrillary acidic protein and vimentin immunoreactivity of astroglial cells in the central nervous system of adult Podarcis sicula (Squamata, Lacertidae). J Anat 198:67–75PubMedCrossRef
go back to reference Lazzari M, Franceschini V (2004) Glial fibrillary acidic protein and vimentin immunoreactivity of astroglial cells in the central nervous system of the African lungfish, Protopterus annectens (Dipnoi: Lepidosirenidae). J Morphol 262:741–749PubMedCrossRef Lazzari M, Franceschini V (2004) Glial fibrillary acidic protein and vimentin immunoreactivity of astroglial cells in the central nervous system of the African lungfish, Protopterus annectens (Dipnoi: Lepidosirenidae). J Morphol 262:741–749PubMedCrossRef
go back to reference Lazzari M, Franceschini V (2005) Astroglial Cells in the Central Nervous System of the Brown Anole Lizard, Anolis sagrei, revealed by Intermediate Filament Immunohistochemistry. J Morphol 265:325–334PubMedCrossRef Lazzari M, Franceschini V (2005) Astroglial Cells in the Central Nervous System of the Brown Anole Lizard, Anolis sagrei, revealed by Intermediate Filament Immunohistochemistry. J Morphol 265:325–334PubMedCrossRef
go back to reference Lazzari M, Franceschini V, Ciani F (1997) Glial Fibrillary Acidic Protein and Vimentin in radial glia of Ambystoma mexicanum and Triturus carnifex: An immunocytochemical Study. J Brain Res 38:187–194 Lazzari M, Franceschini V, Ciani F (1997) Glial Fibrillary Acidic Protein and Vimentin in radial glia of Ambystoma mexicanum and Triturus carnifex: An immunocytochemical Study. J Brain Res 38:187–194
go back to reference Levitt P, Rakic P (1980) Immunoperoxidase localization of glial fibrillary acidic protein in radial glial cells and astrocytes of the developing rhesus monkey brain. J Comp Neurol 193:815–840PubMedCrossRef Levitt P, Rakic P (1980) Immunoperoxidase localization of glial fibrillary acidic protein in radial glial cells and astrocytes of the developing rhesus monkey brain. J Comp Neurol 193:815–840PubMedCrossRef
go back to reference Miller RH, Liuzzi FJ (1986) Regional specialization of the radial glial cells of the adult frog spinal cord. J Neurocytol 15:187–196PubMedCrossRef Miller RH, Liuzzi FJ (1986) Regional specialization of the radial glial cells of the adult frog spinal cord. J Neurocytol 15:187–196PubMedCrossRef
go back to reference Monzon-Mayor M, Yanes C, Ghandour MS, De Barry J, Gombos G (1990) GFAP and vimentin immunohistochemistry in the adult and developing midbrain of the lizard Gallotia galloti. J Comp Neurol 295:569–579PubMedCrossRef Monzon-Mayor M, Yanes C, Ghandour MS, De Barry J, Gombos G (1990) GFAP and vimentin immunohistochemistry in the adult and developing midbrain of the lizard Gallotia galloti. J Comp Neurol 295:569–579PubMedCrossRef
go back to reference Monzon-Mayor M, Yanes C, De Barry J, Capdevilla-Carbonell C, Renau-Piqueras J, Tholey G, Gombos G (1998) Heterogeneous immunoreactivity in glial cells in the mesencephalon of a lizard: a double labelling immunohistochemical study. J Morphol 235:109–119PubMedCrossRef Monzon-Mayor M, Yanes C, De Barry J, Capdevilla-Carbonell C, Renau-Piqueras J, Tholey G, Gombos G (1998) Heterogeneous immunoreactivity in glial cells in the mesencephalon of a lizard: a double labelling immunohistochemical study. J Morphol 235:109–119PubMedCrossRef
go back to reference Naujoks-Manteuffel C, Meyer DL (1996) Glial fibrillary acidic protein in the brain of the caecilian Typhlonectes natans (Amphibia, Gymnophiona): an immunocytochemical study. Cell Tissue Res 283:51–58 Naujoks-Manteuffel C, Meyer DL (1996) Glial fibrillary acidic protein in the brain of the caecilian Typhlonectes natans (Amphibia, Gymnophiona): an immunocytochemical study. Cell Tissue Res 283:51–58
go back to reference Onteniente B, Kimura H, Maeda T (1983) Comparative study of the glial fibrillary acidic protein in vertebrates by PAP immunohistochemistry. J Comp Neurol 215:427–436PubMedCrossRef Onteniente B, Kimura H, Maeda T (1983) Comparative study of the glial fibrillary acidic protein in vertebrates by PAP immunohistochemistry. J Comp Neurol 215:427–436PubMedCrossRef
go back to reference Oudega M, Marani E (1991) Expression of vimentin and glial fibrillary acidic protein in the developing rat spinal cord: an immunocytochemical study of the spinal cord glial system. J Anat 179:97–114PubMed Oudega M, Marani E (1991) Expression of vimentin and glial fibrillary acidic protein in the developing rat spinal cord: an immunocytochemical study of the spinal cord glial system. J Anat 179:97–114PubMed
go back to reference Pixley SK, De Vellis J (1984) Transition between immature radial glia and mature astrocytes studied with a monoclonal antibody to vimentin. Brain Res 317:201–209PubMed Pixley SK, De Vellis J (1984) Transition between immature radial glia and mature astrocytes studied with a monoclonal antibody to vimentin. Brain Res 317:201–209PubMed
go back to reference Pulido-Caballero J, Jiménez-Sampedro F, Echevarría-Aza D, Martínez-Millán L (1994) Postnatal development of vimentin-positive cells in the rabbit superior colliculus. J Comp Neurol 343:102–112PubMedCrossRef Pulido-Caballero J, Jiménez-Sampedro F, Echevarría-Aza D, Martínez-Millán L (1994) Postnatal development of vimentin-positive cells in the rabbit superior colliculus. J Comp Neurol 343:102–112PubMedCrossRef
go back to reference Rubio M, Suarez I, Bodega G, Fernandez B (1992) Glial fibrillary acidic protein and vimentin immunohistochemistry in the posterior rhombencephalon of the Iberian barb (Barbus comiza). Neurosci Lett 134:203–206PubMedCrossRef Rubio M, Suarez I, Bodega G, Fernandez B (1992) Glial fibrillary acidic protein and vimentin immunohistochemistry in the posterior rhombencephalon of the Iberian barb (Barbus comiza). Neurosci Lett 134:203–206PubMedCrossRef
go back to reference Szaro BG, Gainer H (1988) Immunocytochemical identification of non-neuronal intermediate filament proteins in the developing Xenopus laevis nervous system. Dev Brain Res 43:207–224CrossRef Szaro BG, Gainer H (1988) Immunocytochemical identification of non-neuronal intermediate filament proteins in the developing Xenopus laevis nervous system. Dev Brain Res 43:207–224CrossRef
go back to reference Tapscott SJ, Bennett GS, Toyama Y, Kleinbart F, Holtzer H (1981) Intermediate filament protein in the developing chick spinal cord. Dev Biol 86:40–54PubMedCrossRef Tapscott SJ, Bennett GS, Toyama Y, Kleinbart F, Holtzer H (1981) Intermediate filament protein in the developing chick spinal cord. Dev Biol 86:40–54PubMedCrossRef
go back to reference Voigt T (1989) Development of glial cells in the cerebral walls of ferrets: direct tracing of their transformation from radial glia into astrocytes. J Comp Neurol 289:74–88PubMedCrossRef Voigt T (1989) Development of glial cells in the cerebral walls of ferrets: direct tracing of their transformation from radial glia into astrocytes. J Comp Neurol 289:74–88PubMedCrossRef
go back to reference Wasowicz M, Pierre J, Reperant J, Ward R, Vesselkin NP, Versaux-Botteri C (1994) Immunoreactivity to glial fibrillary acidic protein (GFAP) in the brain and spinal cord of the lamprey (Lampetra fluviatilis). J Brain Res 35:71–78 Wasowicz M, Pierre J, Reperant J, Ward R, Vesselkin NP, Versaux-Botteri C (1994) Immunoreactivity to glial fibrillary acidic protein (GFAP) in the brain and spinal cord of the lamprey (Lampetra fluviatilis). J Brain Res 35:71–78
go back to reference Wicht H, Derouiche A, Korf H-W (1994) An immunocytochemical investigation of glial morphology in the Pacific hagfish: radial and astrocyte-like glia have the same phylogenetic age. J Neurocytol 23:565–576PubMedCrossRef Wicht H, Derouiche A, Korf H-W (1994) An immunocytochemical investigation of glial morphology in the Pacific hagfish: radial and astrocyte-like glia have the same phylogenetic age. J Neurocytol 23:565–576PubMedCrossRef
go back to reference Yamada K, Watanabe M (2002) Cytodifferentiation of Bergmann glia and its retationship with Purkinje cells. Anat Sci Int 77:94–108PubMedCrossRef Yamada K, Watanabe M (2002) Cytodifferentiation of Bergmann glia and its retationship with Purkinje cells. Anat Sci Int 77:94–108PubMedCrossRef
go back to reference Yamada T, Kawamata T, Walker DG, Mcgeer PL (1992) Vimentin immunoreactivity in normal and pathological human brain tissue. Acta Neuropathol 84:157–162PubMedCrossRef Yamada T, Kawamata T, Walker DG, Mcgeer PL (1992) Vimentin immunoreactivity in normal and pathological human brain tissue. Acta Neuropathol 84:157–162PubMedCrossRef
go back to reference Yanes C, Monzon-Mayor M, Ghandour MS, De Barry J, Gombos G (1990) Radial glia and astrocytes in developing and adult telencephalon of the lizard Gallotia galloti as revealed by immunohistochemistry with anti-GFAP and anti-vimentin antibodies. J Comp Neurol 295:559–568PubMedCrossRef Yanes C, Monzon-Mayor M, Ghandour MS, De Barry J, Gombos G (1990) Radial glia and astrocytes in developing and adult telencephalon of the lizard Gallotia galloti as revealed by immunohistochemistry with anti-GFAP and anti-vimentin antibodies. J Comp Neurol 295:559–568PubMedCrossRef
go back to reference Zamora AJ, Mutin M (1988) Vimentin and glial fibrillary acidic protein filaments in radial glia of the adult urodele spinal cord. Neuroscience 27:279–288PubMedCrossRef Zamora AJ, Mutin M (1988) Vimentin and glial fibrillary acidic protein filaments in radial glia of the adult urodele spinal cord. Neuroscience 27:279–288PubMedCrossRef
Metadata
Title
Intermediate filament immunohistochemistry of astroglial cells in the leopard gecko, Eublepharis macularius
Authors
Maurizio Lazzari
Valeria Franceschini
Publication date
01-11-2005
Publisher
Springer-Verlag
Published in
Brain Structure and Function / Issue 4/2005
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI
https://doi.org/10.1007/s00429-005-0049-x

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