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01-07-2006 | Original Article

Expression of HLA-G in malignant mesothelioma and clinically aggressive breast carcinoma

Authors: Lilach Kleinberg, Vivi Ann Flørenes, Martina Skrede, Hiep Phuc Dong, Søren Nielsen, Michael T. McMaster, Jahn M. Nesland, Ie-Ming Shih, Ben Davidson

Published in: Virchows Archiv | Issue 1/2006

Abstract

The aim of the present study was to evaluate HLA-G expression in breast carcinoma and malignant mesothelioma (MM). Malignant breast carcinoma effusions (46) and corresponding solid tumors (39) and 104 MM (26 effusions, 78 solid tumors) were analyzed using immunohistochemistry (IHC). HLA-G protein and mRNA expression were further studied using immunoblotting (IB) and RT-PCR. HLA-ABC expression was analyzed using flow cytometry (FCM). IHC showed predominantly focal HLA-G expression in 12 of 46 (26%) breast carcinoma effusions and 16 of 39 (41%) solid lesions. In MM, 20 of 78 (26%) solid lesions and 14 of 26 (54%) effusions were focally HLA-G positive. Expression in MM was higher in effusions (p=0.008). IB showed more frequent HLA-G expression in MM compared with breast carcinoma effusions, while RT-PCR showed HLA-G mRNA expression in both tumors. FCM showed conserved HLA-ABC expression in 15 of 15 effusions. Breast cancer patients with HLA-G-positive tumor cells had shorter disease-free survival (mean 37 vs 85, median 25 vs 31 months), though not significantly (p=0.14). In conclusion, HLA-G is focally expressed in MM and breast carcinoma, while HLA-ABC expression is conserved. However, the up-regulated expression of HLA-G in MM effusions and its possible association with shorter disease-free survival in advanced stage of breast carcinoma suggest a possible role in immune response evasion in some tumors.

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Title: Expression of HLA-G in malignant mesothelioma and clinically aggressive breast carcinoma
Authors: Lilach Kleinberg
Vivi Ann Flørenes
Martina Skrede
Hiep Phuc Dong
Søren Nielsen
Michael T. McMaster
Jahn M. Nesland
Ie-Ming Shih
Ben Davidson
Publication date: 01-07-2006
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 1/2006
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-005-0144-7

At a glance: The STEP trials

Springer Medicine

Expression of HLA-G in malignant mesothelioma and clinically aggressive breast carcinoma

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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