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Langenbeck's Archives of Surgery

Published in:

01-11-2010 | Original Article

Microvascular response to calcium phosphate bone substitutes: an intravital microscopy analysis

Authors: Bernd Roetman, Andrej Ring, Stefan Langer, Thomas A. Schildhauer, Gert Muhr, Manfred Köller

Published in: Langenbeck's Archives of Surgery | Issue 8/2010

Abstract

Objectives

The purpose was to evaluate inflammatory and microcirculatory reactions after implantation of various calcium phosphate bone substitutes in an in vivo model.

Methods

Calcium phosphate-based bone substitutes were implanted in dorsal skinfold chambers of mice. Intravital fluorescence microscopy was performed to measure inflammatory and microcirculatory reactions based on functional vessel density (FVD), capillary leakage, and relative white blood cell velocity (rWBCV).

Results

An increase of FVD was observed in all groups and the capillary leakage grew with a level of significance (p < 0.001). The fraction of rolling and sticking leukocytes (rWBCV) was highest at the beginning of the trial and decreased during the course.

Conclusions

There are differences in microvascular soft tissue reactions between various calcium phosphate bone substitutes, but inflammatory reactions were moderate, and the results revealed no reasons which explain the sporadic failure of the tested substances under clinical conditions.

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Title: Microvascular response to calcium phosphate bone substitutes: an intravital microscopy analysis
Authors: Bernd Roetman
Andrej Ring
Stefan Langer
Thomas A. Schildhauer
Gert Muhr
Manfred Köller
Publication date: 01-11-2010
Publisher: Springer-Verlag
Published in: Langenbeck's Archives of Surgery / Issue 8/2010
Print ISSN: 1435-2443
Electronic ISSN: 1435-2451
DOI: https://doi.org/10.1007/s00423-010-0608-3

At a glance: The STEP trials

Springer Medicine

Microvascular response to calcium phosphate bone substitutes: an intravital microscopy analysis

Abstract

Objectives

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Methods

Results

Conclusions

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