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01-08-2021 | Original Article

Fifteen days of moderate normobaric hypoxia does not affect mitochondrial function, and related genes and proteins, in healthy men

Authors: Alessandra Ferri, Xu Yan, Jujiao Kuang, Cesare Granata, Rodrigo S. F. Oliveira, Christopher P. Hedges, Adriano E. Lima-Silva, Francois Billaut, David J. Bishop

Published in: European Journal of Applied Physiology | Issue 8/2021

Abstract

Purpose

To investigate within the one study potential molecular and cellular changes associated with mitochondrial biogenesis following 15 days of exposure to moderate hypoxia.

Methods

Eight males underwent a muscle biopsy before and after 15 days of hypoxia exposure (FiO₂ = 0.140–0.154; ~ 2500–3200 m) in a hypoxic hotel. Mitochondrial respiration, citrate synthase (CS) activity, and the content of genes and proteins associated with mitochondrial biogenesis were investigated.

Results

Our main findings were the absence of significant changes in the mean values of CS activity, mitochondrial respiration in permeabilised fibers, or the content of genes and proteins associated with mitochondrial biogenesis, after 15 days of moderate normobaric hypoxia.

Conclusion

Our data provide evidence that 15 days of moderate normobaric hypoxia have negligible influence on skeletal muscle mitochondrial content and function, or genes and proteins content associated with mitochondrial biogenesis, in young recreationally active males. However, the increase in mitochondrial protease LON content after hypoxia exposure suggests the possibility of adaptations to optimise respiratory chain function under conditions of reduced O₂ availability.

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Title: Fifteen days of moderate normobaric hypoxia does not affect mitochondrial function, and related genes and proteins, in healthy men
Authors: Alessandra Ferri
Xu Yan
Jujiao Kuang
Cesare Granata
Rodrigo S. F. Oliveira
Christopher P. Hedges
Adriano E. Lima-Silva
Francois Billaut
David J. Bishop
Publication date: 01-08-2021
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 8/2021
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-021-04706-4

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Fifteen days of moderate normobaric hypoxia does not affect mitochondrial function, and related genes and proteins, in healthy men

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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