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01-12-2018 | Original Article

Resistance training in young men induces muscle transcriptome-wide changes associated with muscle structure and metabolism refining the response to exercise-induced stress

Authors: Felipe Damas, Carlos Ugrinowitsch, Cleiton A. Libardi, Paulo R. Jannig, Amy J. Hector, Chris McGlory, Manoel E. Lixandrão, Felipe C. Vechin, Horacio Montenegro, Valmor Tricoli, Hamilton Roschel, Stuart M. Phillips

Published in: European Journal of Applied Physiology | Issue 12/2018

Abstract

Background

Gene expression is an important process underpinning the acute and chronic adaptive response to resistance exercise (RE) training.

Purpose

To investigate the effect of training status on vastus lateralis muscle global transcriptome at rest and following acute RE.

Methods

Muscle biopsies of nine young men (age: 26(2) years; body mass: 69(9) kg; height 172(6) cm) who undertook RE training for 10 weeks were collected pre and 24 h post-RE in the untrained (W1) and trained (W10) states and analysed using microarray. Tests of differential expression were conducted for rested and after RE contrasts in both training states. To control for false discovery rate (FDR), multiple testing correction was performed at a cut-off of FDR < 0.05.

Results

Unaccustomed RE (at W1) upregulated muscle gene transcripts related to stress (e.g., heat shock proteins), damage and inflammation, structural remodelling, protein turnover and increased translational capacity. Trained muscles (at W10) showed changes in the transcriptome signature regarding the regulation of energy metabolism, favouring a more oxidative one, upregulated antioxidant- and immune-related genes/terms, and gene transcripts related to the cytoskeleton and extracellular matrix, muscle contraction, development and growth.

Conclusions

These results highlight that chronic repetition of RE changes muscle transcriptome response towards a more refined response to RE-induced stress.

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Title: Resistance training in young men induces muscle transcriptome-wide changes associated with muscle structure and metabolism refining the response to exercise-induced stress
Authors: Felipe Damas
Carlos Ugrinowitsch
Cleiton A. Libardi
Paulo R. Jannig
Amy J. Hector
Chris McGlory
Manoel E. Lixandrão
Felipe C. Vechin
Horacio Montenegro
Valmor Tricoli
Hamilton Roschel
Stuart M. Phillips
Publication date: 01-12-2018
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 12/2018
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-018-3984-y

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Resistance training in young men induces muscle transcriptome-wide changes associated with muscle structure and metabolism refining the response to exercise-induced stress

Abstract

Background

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Purpose

Methods

Results

Conclusions

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