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01-10-2011 | Original Article

Effect of different resistance-training regimens on the WNT-signaling pathway

Authors: Marcelo Larciprete Leal, Leonardo Lamas, Marcelo Saldanha Aoki, Carlos Ugrinowitsch, Marcela Sorelli Carneiro Ramos, Valmor Tricoli, Anselmo Sigari Moriscot

Published in: European Journal of Applied Physiology | Issue 10/2011

Abstract

The purpose of the present study was to evaluate the effects of 8 weeks of strength and power training on the expression of genes related to the canonical WNT pathway and β-catenin protein levels in physically active men. Twenty-five subjects (27.4 ± 4.6 years) were balanced based on their relative maximum strength in the squat exercise (squat 1RM/body mass) and randomly assigned to strength training (ST) (n = 10), power training (PT) (n = 10), and control (C) (n = 5) groups. The ST and the PT groups performed high and low intensity squats, respectively, thrice a week, for 8 weeks. Muscle biopsies from the vastus lateralis muscle were collected before and after the training period. Relative strength and power increased similarly in both ST and PT groups (P < 0.001). Fiber cross-sectional area also increased similarly in both ST and PT groups. Gene expression and β-catenin protein expression levels were assessed by real-time PCR and Western blot. Certain genes were up-regulated in the ST group (WNT1: 6.4-fold, P < 0.0001; SFRP1: 3.3-fold, P < 0.0001 and LEF1: 7.3-fold, P < 0.0001) and also in the PT group (WNT1: 24.9-fold, P < 0.0001; SFRP1: 2.7-fold, P < 0.0001; LEF1: 34.1-fold, P < 0.0001 and Cyclin D1: 7.7-fold, P < 0.001). In addition, the expression of key WNT pathway genes was substantially more responsive to PT than to ST (WNT1: P < 0.0001; LEF1: P < 0.0001 and Cyclin D1: P < 0.001). Finally, the total β-catenin protein content increased only in the PT group (P < 0.05). Our data indicate that a PT regimen triggers greater responses in key elements of the WNT pathway.

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Title: Effect of different resistance-training regimens on the WNT-signaling pathway
Authors: Marcelo Larciprete Leal
Leonardo Lamas
Marcelo Saldanha Aoki
Carlos Ugrinowitsch
Marcela Sorelli Carneiro Ramos
Valmor Tricoli
Anselmo Sigari Moriscot
Publication date: 01-10-2011
Publisher: Springer-Verlag
Published in: European Journal of Applied Physiology / Issue 10/2011
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-011-1874-7

At a glance: The STEP trials

Springer Medicine

Effect of different resistance-training regimens on the WNT-signaling pathway

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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