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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-12-2019 | Laser | Basic Science

Analysis of corneal stromal roughness after iFS 150 kHz and LenSx femtosecond LASIK flap creation in porcine eyes

Authors: Juan Gros-Otero, Samira Ketabi, Rafael Cañones-Zafra, Montserrat Garcia-Gonzalez, Alberto Parafita-Fernandez, Cesar Villa-Collar, Santiago Casado, Miguel Teus

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 12/2019

Abstract

Purpose

To describe and compare the stromal bed roughness obtained after laser in situ keratomileusis (LASIK) flap creation using a corneal femtosecond laser platform (iFS 150) and a “dual” femtosecond (FS) laser platform (LenSx).

Methods

This ex vivo experimental study was conducted in an animal model using 12 freshly enucleated porcine eyes, six with each femtosecond laser. The standard laser treatment parameters were used for the experiment. After LASIK flap creation, the corneal stromal roughness was assessed using atomic force microscopy (AFM) in contact mode immersed in liquid. In each sample, surface measurements were obtained in 60 regions of six eyes per FS laser in 10 20 × 20-micron areas of the central corneal stroma at 512 × 512-point resolution. The surface roughness was measured and the root-mean-square (RMS) values of the roughness were obtained.

Results

The mean RMS ± standard deviation values were 430 ± 150 nm for the corneal femtosecond laser platform and 370 ± 100 nm for the dual FS laser platform (P < 0.011).

Conclusions

In this experimental study with AFM, we found smoother stromal beds after LASIK flap creation with LenSx compared to iFS 150 kHz. Further studies are needed to understand visual implications of the differences found.

Huhtala A, Pietilä J, Mäkinen P et al (2016) Femtosecond lasers for laser in situ keratomileusis: a systematic review and metaanalysis. Clin Ophthalmol 10:393–404CrossRef

Parafita-Fernandez A, Garcia-Gonzalez M, Katsanos A et al (2019) Two femtosecond LASIK platforms: comparison of valuation of visual acuity, flap thickness and stromal optical density. Cornea 38:98–104CrossRef

Garcia-Gonzalez M, Bouza-Miguens C, Parafita-Fernandez A et al (2018) Comparison of visual outcomes and flap morphology using 2 femtosecond laser platforms. J Cataract Refract Surg 44:78–84CrossRef

Bolivar G, Garcia-Gonzalez M, Laucirika G et al (2019) Intraocular pressure rises during laser in situ keratomileusis: comparison of 3 femtosecond laser platforms. J Cataract Refract Surg. In press. https://doi.org/10.1016/j.jcrs.2019.03.013 CrossRef

Farjo AA, Sugar A, Schallhorn SC et al (2013) Femtosecond lasers for LASIK flap creation: a report by the American Academy of Ophthalmology. Ophthalmology 120:e5–e20CrossRef

Yu C, Manche EE (2015) Comparison of 2 femtosecond lasers for flap creation in myopic laser in situ keratomileusis: one-year results. J Cataract Refract Surg 41:740–748CrossRef

Yu C, Manche EE (2014) A comparison of LASIK flap thickness and morphology between the Intralase 60 and 150kHz femtosecond lasers. J Refract Surg 30:827–830CrossRef

Sales CS, Manche EE (2016) Comparison of self-reported quality of vision outcomes after myopic LASIK with two femtosecond lasers: a prospective, eye-to-eye study. Clin Ophthalmol 10:1691–1699CrossRef

Colombo-Barboza MN, Colombo-Barboza GN, Colombo-Barboza LR et al (2018) Multifunctional femtosecond laser platform and correlation with clinical preoperative measurements. J Cataract Refract Surg 44:811–817CrossRef

10.

Slade S, Ignacio T, Spector S (2018) Evaluation of a multifunctional femtosecond laser for the creation of laser in situ keratomileusis flaps. J Cataract Refract Surg 44:280–286CrossRef

11.

Ji YW, Kim M, Kang DSY et al (2017) Effect of lowering laser energy on the surface roughness of human corneal lenticules in SMILE. J Refract Surg 33:617–624CrossRef

12.

Kymionis GD, Kontadakis GA, Naoumidi I et al (2014) Comparative study of stromal bed of LASIK flaps created with femtosecond lasers (IntraLase FS150, WaveLight FS200) and mechanical microkeratome. Br J Ophthalmol 98:133–137CrossRef

13.

De Santo MP, Lombardo M, Serrao S et al (2004) Atomic force microscopy in ophthalmic surgery. 4th IEEE Conference on Nanotechnology 252–254.

14.

Spadea L, Maraone G, Verboschi F et al (2016) Effect of corneal light scatter on vision: a review of the literature. Int J Ophthalmol 9:459–464PubMedPubMedCentral

15.

Azar DT, Azar FN, Brodie SE et al (2017) Optical considerations in keratorefractive surgery. In: Cantor LB, Rapuano CJ, Cioffi GA (eds) Basic and Clinical Science Course 2017-2018. Section 3. Clinical Optics. American Academy of Ophthalmology, San Francisco, pp 223–236

16.

Soong HK, Malta JB (2009) Femtosecond lasers in ophthalmology. Am J Ophthalmol 147:189–197CrossRef

17.

Vinciguerra P, Azzolin M, Airaghi P et al (1998) Effect of decreasing surface and interface irregularities after photorefractive keratectomy and laser in situ keratomileusis on optical and functional outcomes. J Refract Surg 14:S199–S203PubMed

18.

Moshifar M, Desautels JD, Quist TS et al (2016) Rainbow glare after laser-assisted in situ keratomileusis: a review of the literature. Clin Ophthalmol 10:2245–2249CrossRef

19.

Zhang Y, Chen Y (2018) High incidence of rainbow glare after femtosecond laser assisted-LASIK using the upgraded FS200 femtosecond laser. BMC Ophthalmol 18:71CrossRef

20.

Wilhelm FW, Glessman T, Hanschke R et al (2000) Cut edges and surface characteristics produced by different microkeratomes. J Refract Surg 16:690–700PubMed

21.

Hamill MB, Kohnen T (2002) Scanning electron microscopic evaluation of the surface characteristics of 4 microkeratome systems in human corneas. J Cataract Refract Surg 28:328–336CrossRef

22.

Kermani O, Oberheide U (2008) Comparative micromorphologic in vitro porcine study of Intralase and Femto LDV femtosecond lasers. J Cataract Refract Surg 34:1393–1399CrossRef

23.

Hammer CM, Kunert KS, Zhang Y et al (2018) Interface morphology and gas production by a refractive 347nm ultraviolet femtosecond laser: comparison with established laser systems. J Cataract Refract Surg 44:1371–1377CrossRef

24.

Sarayba MA, Ignacio TS, Binder PS et al (2007) Comparative study of stromal bed quality by using mechanical, IntraLase femtosecond laser 15- and 30-kHz microkeratomes. Cornea 26:446–451CrossRef

25.

Sarayba MA, Ignacio TS, Tran DB et al (2007) A 60 kHz Intralase femtosecond laser creates a smoother LASIK stromal bed surface compared to a Zyoptix XP mechanical microkeratome in human donor eyes. J Refract Surg 23:331–337CrossRef

Title: Analysis of corneal stromal roughness after iFS 150 kHz and LenSx femtosecond LASIK flap creation in porcine eyes
Authors: Juan Gros-Otero
Samira Ketabi
Rafael Cañones-Zafra
Montserrat Garcia-Gonzalez
Alberto Parafita-Fernandez
Cesar Villa-Collar
Santiago Casado
Miguel Teus
Publication date: 01-12-2019
Publisher: Springer Berlin Heidelberg
Keyword: Laser
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 12/2019
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-019-04497-7

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Analysis of corneal stromal roughness after iFS 150 kHz and LenSx femtosecond LASIK flap creation in porcine eyes

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

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