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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-08-2018 | Basic Science

Comparison of pre-retinal oxygen pressure changes after selective retina therapy versus conventional photocoagulation in the rabbit eye

Authors: Jae Ryun Kim, Young-Gun Park, Young Jung Roh

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 8/2018

Abstract

Purpose

To evaluate oxygen consumption by photoreceptors, we estimated changes in pre-retinal oxygen pressure (PO₂) after selective retina therapy (SRT) compared with conventional photocoagulation (PC) in rabbits.

Methods

One eye of each of 10 Chinchilla Bastard rabbits received both 144 laser SRT spots and 144 laser PC spots. Color fundus photography, optical coherence tomography (OCT), and fluorescein angiography were used to evaluate the lesions after treatment. Fiber-optic sensors (optodes) were used to determine the pre-retinal PO₂ levels of untreated, SRT-treated, and PC-treated areas of laser-damaged eyes 7 days after treatment. The pre-retinal PO₂ was measured in the other five eyes (controls). The same procedures were applied to these remaining five eyes 4 weeks after treatment. Light microscopy (LM) was used to evaluate histological changes 7 days and 4 weeks after treatment.

Results

We found no significant difference in the mean pre-retinal PO₂ values among untreated, SRT-treated, and control eyes 7 days after treatment. However, the mean pre-retinal PO₂ value in PC-treated regions (24.3 ± 4.9 mmHg; mean ± SD) was higher than those in untreated regions (17.0 ± 1.8 mmHg; P = 0.019), SRT-treated regions (16.7 ± 2.6 mmHg; P = 0.015), and controls (16.9 ± 2.4 mmHg; P = 0.018). Similarly, the mean pre-retinal PO₂ of only PC-treated regions (25.2 ± 4.7 mmHg) was higher than those of the untreated regions (16.3 ± 2.5 mmHg; P = 0.006), SRT-treated regions (17.7 ± 3.1 mmHg; P = 0.023), and controls (16.4 ± 2.4 mmHg; P = 0.007) 4 weeks after treatment. OCT and LM revealed selective retinal pigment epithelium damage with the sparing of photoreceptors in SRT lesions.

Conclusions

SRT treatment did not induce changes in the oxygen consumption of photoreceptors, or the pre-retinal PO₂.

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Title: Comparison of pre-retinal oxygen pressure changes after selective retina therapy versus conventional photocoagulation in the rabbit eye
Authors: Jae Ryun Kim
Young-Gun Park
Young Jung Roh
Publication date: 01-08-2018
Publisher: Springer Berlin Heidelberg
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 8/2018
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-018-4022-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Comparison of pre-retinal oxygen pressure changes after selective retina therapy versus conventional photocoagulation in the rabbit eye

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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