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Graefe's Archive for Clinical and Experimental Ophthalmology

Published in:

01-08-2017 | Basic Science

Blockade of MMP-2 and MMP-9 inhibits corneal lymphangiogenesis

Authors: Hai-Tao Du, Ling-Ling Du, Xian-Ling Tang, Hong-Yan Ge, Ping Liu

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 8/2017

Abstract

Purpose

To investigate the roles of a selective MMP-2 and -9 inhibitor (SB-3CT) in corneal inflammatory lymphangiogenesis.

Methods

The expression of MMP-2 and -9 in the cornea after suture inplacement, treated with SB-3CT or negative control, was detected by real-time polymerase chain reaction (PCR). Inflammatory corneal neovascularization (NV) was induced by corneal suture placement. Mice were treated with SB-3CT eye drops (twice daily for 1 week, 5 μL per drop; 50, 100, or 200 μM). The outgrowth of blood and lymphatic vessels, and macrophage recruitment were analyzed by immunofluorescence assay. The expressions of vascular endothelial growth factor-C (VEGF-C) and its receptor VEGFR-3 were tested by real-time PCR.

Results

MMP-2 and -9 expression were suppressed significantly by treatment with SB-3CT. The data demonstrated, for the first time, that SB-3CT strongly reduced corneal lymphangiogenesis and macrophage infiltration during inflammation. Furthermore, expressions of VEGF-C and its receptor VEGFR-3 were significantly inhibited by SB-3CT during corneal lymphangiogenesis.

Conclusions

These novel findings indicated that blockade of MMP-2 and -9 could inhibit lymphangiogenesis. Further investigation of this factor may provide novel therapies for transplant rejection and other lymphatic disorders.

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Title: Blockade of MMP-2 and MMP-9 inhibits corneal lymphangiogenesis
Authors: Hai-Tao Du
Ling-Ling Du
Xian-Ling Tang
Hong-Yan Ge
Ping Liu
Publication date: 01-08-2017
Publisher: Springer Berlin Heidelberg
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 8/2017
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-017-3651-8

ACC 2024 Congress

Springer Medicine

Blockade of MMP-2 and MMP-9 inhibits corneal lymphangiogenesis

Abstract

Purpose

Methods

Results

Conclusions

ACC 2024 Congress

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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