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Graefe's Archive for Clinical and Experimental Ophthalmology

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Open Access 01-12-2015 | Basic Science

Elevated vitreous body glial fibrillary acidic protein in retinal diseases

Authors: Anselm Gerhard Maria Jünemann, Robert Rejdak, Cord Huchzermeyer, Ryszard Maciejewski, Pawel Grieb, Friedrich E. Kruse, Eberhart Zrenner, Konrad Rejdak, Axel Petzold

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 12/2015

Abstract

Purpose

Increased expression of glial fibrillary acidic protein (GFAP) is a characteristic of gliotic activation (Müller cells and astrocytes) in the retina. This study assessed vitreous body GFAP levels in various forms of retinal pathology.

Methods

This prospective study included 82 patients who underwent vitrectomy (46 retinal detachments (RDs), 13 macular hole (MHs), 15 epiretinal glioses (EGs), 8 organ donors). An established enzyme–linked immunosorbent assay (ELISA, SMI26) was used for quantification of GFAP.

Results

The highest concentration of vitreous body GFAP in organ donors was 20 pg/mL and it was used as the cutoff. A significant proportion of patients suffering from RD (65 %) to EG (53 %) had vitreous body GFAP levels above this cutoff when compared to organ donors (0 %, p < 0.0001, p = 0.0194, respectively, Fisher’s exact test) and MH (8 %, p < 0.0001, p = 0.0157, respectively). In RD and EG, vitreous body GFAP levels were correlated with axial length (R = 0.69, R = 0.52, p < 0.05 for both).

Conclusions

The data suggest that human vitreous body GFAP is a protein biomarker for glial activation in response to retinal pathologies. Vitreous body GFAP levels may be of interest as a surrogate outcome for experimental treatment strategies in translational studies.

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Title: Elevated vitreous body glial fibrillary acidic protein in retinal diseases
Authors: Anselm Gerhard Maria Jünemann
Robert Rejdak
Cord Huchzermeyer
Ryszard Maciejewski
Pawel Grieb
Friedrich E. Kruse
Eberhart Zrenner
Konrad Rejdak
Axel Petzold
Publication date: 01-12-2015
Publisher: Springer Berlin Heidelberg
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 12/2015
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-015-3127-7

At a glance: The STEP trials

Springer Medicine

Elevated vitreous body glial fibrillary acidic protein in retinal diseases

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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