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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-04-2015 | Retinal Disorders

Early spatiotemporal characterization of microglial activation in the retinas of rats with streptozotocin-induced diabetes

Authors: Xiaofei Chen, Huanfen Zhou, Yan Gong, Shihui Wei, Maonian Zhang

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 4/2015

Abstract

Background

Microglial activation has been recognized as a neuropathological feature in diabetic retinopathy. But the early spatiotemporal characterization of microglial activation in the retina and the optic nerve of diabetic animals has not been fully investigated. The purpose of this study was to investigate early sequential changes of microglia in the retinas of rats with streptozotocin-induced diabetes. Microglia in the optic nerves of rats with streptozotocin-induced diabetes were also studied.

Methods

In 4-week, 8-week, and 12-week diabetic and normal control rats, microglial activation in the retinas and optic nerves was evaluated by immunolabeling with OX-42 antibody. Density, proportion of activation, and laminar distribution of retinal microglia were quantified. The retinal mRNA level of Iba-1, a microglial-specific marker, was measured by real-time PCR.

Results

The density of retinal microglia was not different between diabetic and control rats, but the proportion of activated microglia increased significantly in diabetic rats at each time point. The proportion of microglia increased obviously in the nerve fiber layer and the ganglion cell layer while decreasing in the inner plexiform layer in 12-week diabetic rats. Moreover, retinal Iba-1 mRNA expression increased in 8-week and 12-week diabetic rats. Processes of microglia in the optic nerves of control rats were aligned with the long axis of nerve fibers, while the alignment was disturbed in diabetic rats.

Conclusions

Morphology, proportion of activation, distribution, and mRNA expression of retinal microglia changed characteristically with the progression of the disease in early-stage diabetic rats.

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Title: Early spatiotemporal characterization of microglial activation in the retinas of rats with streptozotocin-induced diabetes
Authors: Xiaofei Chen
Huanfen Zhou
Yan Gong
Shihui Wei
Maonian Zhang
Publication date: 01-04-2015
Publisher: Springer Berlin Heidelberg
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 4/2015
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-014-2727-y

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Early spatiotemporal characterization of microglial activation in the retinas of rats with streptozotocin-induced diabetes

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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