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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-05-2004 | Short Communication

Protein expression pattern of collagen type XV in mouse cornea

Authors: Shizuya Saika, Yuka Okada, Takeshi Miyamoto, Osamu Yamanaka, Yoshitaka Ohnishi, Akio Yamanaka, Akira Ooshima

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 5/2004

Abstract

Purpose

To examine the alteration of protein expression pattern of collagen type XV in cornea during embryonic development and adult tissue repair. Collagen type XV is a basement membrane collagen of a subfamily of multiplexins (multiple triple helix domains and interruptions). Its COOH-terminal peptide has an anti-angiogenic effect and its distribution in avascular tissue of cornea is of interest.

Methods

Eyes of mouse embryos [day (E) 10.5–18.5] and healing adult mouse corneas following either débridement injury or incision were embedded in paraffin. Deparaffinized sections were processed for immunofluorescent staining with anti-collagen XV antibody.

Results

At E14.5 embryonic corneal epithelium, as well as fibroblasts in eyelids, began to express this collagen type very faintly, and at E18.5, besides corneal epithelial expression, epidermis, palpebral conjunctiva, and keratocytes started to express collagen type XV. In adult mouse cornea, collagen type XV was observed in basal and suprabasal epithelial cells and stroma, but not in the subepithelial basement membrane. Healing epithelial cells following débridement or incision injury down-regulated its protein expression.

Conclusions

Mouse embryonic corneal epithelium and keratocytes begin to express collagen type XV before birth. Healing murine corneal epithelium down-regulates collagen XV expression. The presence of collagen XV in corneal stroma may play a part in avascularity.

Chakravarti S, Magnuson T, Lass JH, Jepsen KJ, LaMantia C, Carroll H (1998) Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican. J Cell Biol 141:1277–1286PubMed

Fukai N, Apte SS, Olsen BR (1994) Nonfibrillar collagens. Methods Enzymol 245:3–28PubMed

Halfter W, Dong S, Schurer B, Cole GJ (1998) Collagen XVIII is a basement membrane heparan sulfate proteoglycan. J Biol Chem 278:25404–25412CrossRef

Högg PM, Högg PO, Peltonen S. Autio-Harmainen H, Pihlajaniemi T (1997) Location of type XV collagen in human tissues and its accumulation in the interstitial matrix of the fibrotic kidney. Am J Pathol 150:2075–2086PubMed

Ishizaki M, Zhu G, Haseba T, Shafer SS, Kao WW-Y (1993) Expression of collagen I, smooth muscle α-actin, and vimentin during the healing of alkali-burned and lacerated corneas. Invest Ophthalmol Vis Sci 34:3320–3328PubMed

Kao WW-Y, Ishizaki M (1998) Phenotypic changes of cell activity during corneal wound healing. In: Nishida T (ed) Proceedings: Corneal healing responses to injuries and refractive surgeries. Kugler, The Hague, pp 11–29

Kato T, Chang J-H, Azar DT (2003) Expression of type XVIII collagen during healing of corneal incisions and keratectomy wounds. Invest Ophthalmol Vis Sci 44:78–85PubMed

Kivirikko S, Saarela J, Mayers JC, Autio-Harmainen H, Pihlajaniemi T (1995) Distribution of type XV collagen transcripts in human tissue and their production by muscle cells and fibroblasts. Am J Pathol 147:1500–1509PubMed

Li D, Clark CC, Mayers JC (2000) Basement membrane zone type XV collagen is a disulfide-bonded chondroitin sulfate proteoglycan in human tissues and cultured cells. J Biol Chem 275:22339–22347CrossRefPubMed

10.

Matsubara M, Girard MT, Kublin CL, Cintron C, Fini ME (1991) Differential roles for two gelatinolytic enzymes of the matrix metalloproteinase family in the remodeling cornea. Dev Biol 147:425–439

11.

Muragaki Y, Abe N, Ninomiya Y, Olsen BR, Ooshima A (1994) The human a1(XV) collagen chain contains a large amino-terminal non-triple helical domain with a tendem repeat structure and homology to α1(XVIII) collagen. J Biol Chem 269:4042–4046PubMed

12.

Muragaki Y, Timmons S, Griffith CM, Oh SP, Fadel B, Quertermous T, Olsen BR (1995) Mouse Col18a1 is expressed in a tissue-specific manner as three alternative variants and is localized in basement membrane zones. Proc Natl Acad Sci USA 92:8763–8767PubMed

13.

Myers JC, Kivirikko S, Gordon MK, Dion AS, Pihlajaniemi T (1992) Identification of a previously unknown human collagen chain, α1(XV), characterized by extensive interruptions in the triple-helical region. Proc Natl Acad Sci USA 89:10144–10148PubMed

14.

Myers JC, Dion A, Abraham V, Amenta PS (1996) Type XV collagen exhibits a widespread distribution in human tissues but a distinct localization in basement membrane zones. Cell Tissue Res 286:493–505CrossRefPubMed

15.

Oh SP, Kamagata Y, Muragaki Y, Timmons S, Ooshima A, Olsen BR (1994) Isolation and sequencing of cDNA for proteins with multiple domains of Gly-Xaa-Yaa repeats identify a distinct family of collagenous proteins. Proc Natl Acad Sci USA 91:4229–4233PubMed

16.

Saika S (1998) Alterations in extracellular matrix in experiemntal alkali burn of the cornea. In: Nishida T (ed) Proceedings: Corneal healing responses to injuries and refractive surgeries. Kugler, The Hague, pp 89–101

17.

Saika S, Kobata S, Hashizume N, Okada Y, Yamanaka O (1993) Epithelial basement membrane in alkali-burned corneas in rats. Cornea 12:383–390PubMed

18.

Saika S, Shiraishi A, Saika S, Liu C-Y, Funderburgh JL, Kao CW-C, Converse RL, Kao WW-Y (2000) Role of lumican in the corneal epithelium during wound healing. J Biol Chem 275:2607–2612PubMed

19.

Saika S, Saika S, Liu C-Y, Azhar M, Sanford LP, Doetschman T, Gendron RL, Kao CW-C, Kao WW-Y (2001) TGFβ2 in corneal morphogenesis during mouse embryonic development. Dev Biol 240:419–432CrossRefPubMed

20.

Sasaki T, Fukai N, Mann K, Göhring W, Olsen BR, Timple R (1998) Structure, function and tissue forms of the C-terminal globular domain of collagen XVIII containing the angiogenesis inhibitor endostatin. EMBO J 17:4249–4256CrossRefPubMed

21.

Sasaki T, Larsson H, Tisi D, Claesson-Welsh L, Hohenester E, Timpl R (2000) Endostatins derived from collagens XV and XVIII differ in structural and binding properties, tissue distribution and anti-angiogenic activity. J Mol Biol 301:1179–1190CrossRefPubMed

22.

Sundarraj N, Fite D, Belak R, Sundarraj S, Rada J, Okamoto S, Hassell J (1998) Proteoglycan distribution during healing of corneal stromal wounds in chick. Exp Eye Res 67:433-442PubMed

23.

Tomono Y, Naito I, Ando K, Yonezxawa T, Sado Y, Hirakawa S, Arata J, Okigaki T, Ninomiya Y (2002) Epitope-defined monoclonal antibodies against multiplexin collagens demonstrate that type XV and type XVIII collagens are expressed in specialized basement membranes. Cell Struct Funct 27:9–20CrossRefPubMed

Title: Protein expression pattern of collagen type XV in mouse cornea
Authors: Shizuya Saika
Yuka Okada
Takeshi Miyamoto
Osamu Yamanaka
Yoshitaka Ohnishi
Akio Yamanaka
Akira Ooshima
Publication date: 01-05-2004
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 5/2004
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-003-0854-y

At a glance: The STEP trials

Springer Medicine

Protein expression pattern of collagen type XV in mouse cornea

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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