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Journal of Neurology

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01-11-2019 | Polyneuropathy | Original Communication

Reduced gene expression of netrin family members in skin and sural nerve specimens of patients with painful peripheral neuropathies

Authors: Anna-Lena Schubert, Melissa Held, Claudia Sommer, Nurcan Üçeyler

Published in: Journal of Neurology | Issue 11/2019

Abstract

Objective

To investigate the expression of axon guidance cues in skin and sural nerve biopsies of patients with polyneuropathies (PNP) as potential markers of nerve de- and regeneration and inflammation.

Methods

We prospectively recruited 88 patients with PNP and compared data between patient subgroups and healthy controls. All patients underwent skin punch and/or sural nerve biopsy at the lower leg and proximal thigh. We characterized gene expression profiles of netrin family members as target genes involved in neuronal de- and regeneration [netrin 1, deleted in colorectal cancer (DCC), uncoordinated5H2, neogenin 1 (NEO1), netrin G1, netrin G2] using quantitative real-time PCR.

Results

Gene expression of netrin 1 (p < 0.05 in proximal skin), DCC (p < 0.001 in distal skin), NEO1 (p < 0.05 in distal skin), netrin G1 (p < 0.05 in proximal and p < 0.01 in distal skin), and netrin G2 (p < 0.001 in distal skin) was lower in skin biopsies of patients with neuropathy compared to healthy controls. Gene expression of NEO1 (p < 0.05 in distal skin), netrin G2 (p < 0.05 in distal skin), and DCC (p < 0.05 in sural nerve) was lower in samples of patients with painful compared to painless PNP and also correlated positively with intraepidermal nerve fiber density. Skin and sural nerve gene expression of the investigated target genes did not differ between neuropathies of different etiologies.

Conclusion

We show reduced cutaneous and neural axon guide expression, which may contribute to a dysregulation of nerve fiber de- and regeneration.

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Title: Reduced gene expression of netrin family members in skin and sural nerve specimens of patients with painful peripheral neuropathies
Authors: Anna-Lena Schubert
Melissa Held
Claudia Sommer
Nurcan Üçeyler
Publication date: 01-11-2019
Publisher: Springer Berlin Heidelberg
Keyword: Polyneuropathy
Published in: Journal of Neurology / Issue 11/2019
Print ISSN: 0340-5354
Electronic ISSN: 1432-1459
DOI: https://doi.org/10.1007/s00415-019-09496-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Reduced gene expression of netrin family members in skin and sural nerve specimens of patients with painful peripheral neuropathies

Abstract

Objective

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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