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01-09-2018 | Original Article

Zornia latifolia: a smart drug being adulterated by Stylosanthes guianensis

Authors: L. Cornara, A. P. Fortuna-Perez, I. Bruni, A. Salis, G. Damonte, B. Borghesi, M. Clericuzio

Published in: International Journal of Legal Medicine | Issue 5/2018

Abstract

Dried herbal preparations, based on “Zornia latifolia,” are commonly sold on web, mainly for their supposed hallucinogenic properties. In this work, we demonstrate that these commercial products contain a different Fabacea, i.e., Stylosanthes guianensis, a cheaper plant, widely cultivated in tropical regions as a fodder legume. We were provided with plant samples of true Zornia latifolia from Brazil, and carried out a thorough comparison of the two species. The assignment of commercial samples was performed by means of micro-morphological analysis, DNA barcoding, and partial phytochemical investigation. We observed that Z. latifolia contains large amounts of flavonoid di-glycosides derived from luteolin, apigenin, and genistein, while in S. guianensis lesser amounts of flavonoids, mainly derived from quercetin, were found. It is likely that the spasmolytic and anxiolytic properties of Z. latifolia, as reported in traditional medicine, derive from its contents in apigenin and/or genistein.

From a taxonomical point of view, Fortuna-Perez & Tozzi [4] established under synonymy of Z. latifolia Sm. the following taxa: Z. gemella Willd. ex Vogel (an invalid name), Zornia gracilis DC. (under Z. diphylla (L.) Pers. var. gracilis (DC.) Benth.), Z. diphylla (L.) Pers. var. bernardinensis Chodat & Hassl., Z. maranhamensis G. Don, and Z. surinamensis Miq.. These taxa were frequently identified in herbaria as Z. latifolia. Before this, Zornia latifolia (as Z. latifolia DC.) had already been considered as a variety of Z. diphylla (L.) Pers. by Bentham [5], but Mohlenbrock [6] reestablished the valid name for Z. latifolia Sm..

Zuba D, Byrska B, Maciow M (2011) Comparison of “herbal highs” composition. Anal Bioanal Chem 400:119–126. https://doi.org/10.1007/s00216-011-4743-7. CrossRefPubMed

Cornara L, Borghesi B, Canali C, Andrenacci M, Basso M, Federici S, Labra M (2013) Smart drugs: green shuttle or real drug? Int J Legal Med 127(6):1109–1123CrossRefPubMed

Auwärter V, Dresen S, Weinmann W, Müller M, Pütz M, Ferreirós N (2009) “Spice” and other herbal blends: harmless incense or cannabinoid designer drugs? J Mass Spectrom 44(5):832–837. https://doi.org/10.1002/jms.1558 CrossRefPubMed

Fortuna-Perez AP, Tozzi AMGA (2011) Nomenclatural changes for Zornia (Leguminosae, Papilionoideae, Dalbergieae) in Brazil. Novon 21(3):331–337. https://doi.org/10.3417/2010040 CrossRef

Bentham G (1859) Papilionaceae. Pp. 80–85 in C. F. P. de Martius & A. G. Eichler (editors), Flora Brasiliensis, Vol. 15, Parte 1. F. Fleischer, Leipzig

Mohlenbrock R (1961) A monograph of the leguminous genus Zornia. Webbia 16(1):1–141. https://doi.org/10.1080/00837792.1961.10669720 CrossRef

Sedefov R, Gallegos A, King L, Lopez D, Auwarter V, Hughes B, Griffiths P (2009) Understanding the spice phenomenon. European Monitoring Centre for Drugs and Drug Addiction (EMCDDA) Portugal

Schultes RE, Hofmann A (1979). Plants of the Gods, McGraw-Hill, New York. Reprinted in 1992, Healing arts, Rochester

Von Reis S, Lipp FJ (1982) New plant sources for drugs and foods from the NY botanical garden herbarium. Harvard University Press

10.

Schultes RE, Farnsworth NR (1980) Ethnomedical, botanical and phytochemical aspects of natural hallucinogens. Bot Mus Leafl Harv Univ 28(2):123–214

11.

López J (1981) Isolation of coumarin in Zornia diphylla L. Ing Cienc Quim 5:96–97

12.

Igwe SA, Okawa ANC, Akunyili DN (2001) Preliminary phytochemical and pharmacological studies of Zornia latifolia extracts. Journal of Health and Visual Science 3:12–19

13.

Rojas A, Rojas JI et al (1999) Spasmolytic activity of some plants used by the Otomi Indians of Queretaro (Mexico) for the treatment of gastrointestinal disorders. Phytomedicine 6(5):367–371. https://doi.org/10.1016/S0944-7113(99)80061-0 CrossRefPubMed

14.

Arunkumar R, Ajikumaran Nair S, Subramoniam A (2012) Effectiveness of Zornia diphylla (L.) Pers, against fungal diseases. Ann Phytomed 1:81–89

15.

Khare CP (2007) Indian medicinal plants: an illustrated dictionary. Springer-Verlag, Heidelberg

16.

Geetha KM, Bhavya S, Murugan V (2012) Anticonvulsant activity of the methanolic extract of whole plant of Zornia diphylla (Linn) Pers. J Pharm Res 5(7):3670–3672

17.

DeFilipps RA, Maina SL, Crepin J (2004) Medicinal plants of the Guianas (Guyana, Surinam, French Guiana). Natural Museum of Natural History. Smithsonian Institution, Washington DC

18.

Clericuzio M, Burlando B, Borghesi B, Salis A, Damonte G, Ribulla S, Cornara L (2017) Antiproliferative hydroxy-fatty acids from the fodder legume Stylosanthes guianensis. J Pharm Biomed Anal 141:157–164CrossRefPubMed

19.

Pearse AG (1985) Histochemistry, Theoretical and applied, analytical technology. 4th Ed. Churchill Livingstone, New York

20.

O’Brien TP, Feder N, Mccully ME (1964) Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma 59:368–373CrossRef

21.

Johansen DA (1940) Plant microtechnique. McGraw–Hill Book Co., New York

22.

Lillie RD (1965) Histopathologic technic and practical histochemistry. McGraw–Hill Book Co, New York

23.

Gerlach D (1969) Botanische Mikrotechnik: Eine Einführung. Georg Thieme, Stuttgart

24.

Meira RMSA, Martins FM (2003) Técnica de inclusão de material herborizado em historesina. Rev Árvore 27(1):109–112. https://doi.org/10.1590/S0100-67622003000100015 CrossRef

25.

Vidal BC (1977) Acid glycosaminoglycans and endochondral ossification: microespectrophotometric evaluation and macromolecular orientation. Cell Mol Biol 22:45–64

26.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3):403–410. https://doi.org/10.1016/S0022-2836(05)80360-2 CrossRefPubMed

27.

Mezzasalma V, Ganopoulos I, Galimberti A, Cornara L, Ferri E, Labra M (2017) Poisonous or non-poisonous plants? DNA-based tools and applications for accurate identification. Int J Legal Med 131(1):1–19. https://doi.org/10.1007/s00414-016-1460-y CrossRefPubMed

28.

Fay MF, Bayer C, Alverson WS, de Bruijn AY, Chase MW (1998) Plastid rbcL sequence data indicate a close affinity between Diegodendron and Bixa. Taxon 47(1):43–50. https://doi.org/10.2307/1224017 CrossRef

29.

Dunning LT, Savolainen V (2010) Broad-scale amplification of matK for DNA barcoding plants, a technical note. Bot J Linn Soc 164(1):1–9. https://doi.org/10.1111/j.1095-8339.2010.01071.x CrossRef

30.

Newmaster SG, Ragupathy S, Janovec J (2009) A botanical renaissance: state-of the- art DNA barcoding facilitates an automated identification technology system for plants. Int J Comput App Technol 35(1):50–60. https://doi.org/10.1504/IJCAT.2009.024595 CrossRef

31.

Kress WJ, Wurdack KJ, Zimmer EA, Weigt LA, Janzen DH (2005) Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci U S A 102(23):8369–8374. https://doi.org/10.1073/pnas.0503123102 CrossRefPubMedPubMedCentral

32.

Brubaker CL, Horner HT (1989) Development of epidermal crystals in leaflets of Stylosanthes guianensis (Leguminosae; Papilionoideae). Can J Bot 67(6):1664–1670. https://doi.org/10.1139/b89-210 CrossRef

33.

Da Silva Matos D, Leme FM, Dias ES, Arruda RCO (2013) Anatomia foliar de três espécies de Stylosanthes SW. e sua associação com a composição e formação potencial de fitobezoares em bovinos. Ciência Rural 43(11):2049–2055. https://doi.org/10.1590/S0103-84782013001100021 CrossRef

34.

Fortuna-Perez AP, Castro MM, Tozzi AMGA (2012) Leaflet secretory structures of five taxa of the genus Zornia J.F. Gmel. (Leguminosae, Papilionoideae, Dalbergieae) and their systematic significance. Plant Syst Evol 298(8):1415–1424. https://doi.org/10.1007/s00606-012-0647-z CrossRef

35.

Pushpa B, Dayal R (1993) A flavone glycoside from Dalbergia stipulacea leaves. Phytochemistry 33(3):731–732CrossRef

36.

De Mattia F, Bruni I, Galimberti A, Cattaneo F, Casiraghi M, Labra M (2011) A comparative study of different DNA barcoding markers for the identification of some members of Lamiaceae. Food Rev Int 44(3):693–702. https://doi.org/10.1016/j.foodres.2010.12.032 CrossRef

37.

De Mattia F, Gentili R, Bruni I, Galimberti A, Sgorbati S, Casiraghi M, Labra M (2012) A multi-marker DNA barcoding approach to save time and resources in vegetation surveys. Bot J Linn Soc 169:518–529CrossRef

38.

Viola H, Wasowski C, Stein MLD, Wolfman C, Silveira R, Dajas F, Medina JH, Paladini AC (1995) Apigenin, a component of Matricaria recutita flowers, is a central benzodiazepines receptors-ligand with anxiolytic effects. Planta Med 61(03):213–216. https://doi.org/10.1055/s-2006-958058 CrossRefPubMed

39.

Suresh K, Anupam S (2006) Apigenin: the anxiolytic constituent of Turnera aphrodisiaca. Pharm Biol 44(2):84–90CrossRef

40.

Salgueiro JB, Ardenghi P, Dias M, Ferreira MBC, Izquierdo I, Medina JH (1997) Anxyolitic natural and synthetic flavonoid ligands of the central benzodiazepine receptor have no effect on memory tasks in rats. Pharmacol Biochem Behav 58(4):887–891. https://doi.org/10.1016/S0091-3057(97)00054-3 CrossRefPubMed

41.

Rodrìguez-Landa JF, Hernàndez-Figueroa JD, Hernandez-Calderón BC, Saavedra M (2009) Anxiolytic-like effect of phytoestrogen genistein in rats with long-term absence of ovarian hormones in the black and white model. Prog Neuro-Psychopharmacol Biol Psychiatry 33(2):367–372. https://doi.org/10.1016/j.pnpbp.2008.12.024 CrossRef

42.

Huo X-J, Liu W, Qiu M-H, Huang Z-L, Qu W-M (2012) Genistein induces non-rapid eye movement sleep in mice. Sleep Biol Rhytms 10(4):278–286. https://doi.org/10.1111/j.1479-8425.2012.00571.x CrossRef

Title: Zornia latifolia: a smart drug being adulterated by Stylosanthes guianensis
Authors: L. Cornara
A. P. Fortuna-Perez
I. Bruni
A. Salis
G. Damonte
B. Borghesi
M. Clericuzio
Publication date: 01-09-2018
Publisher: Springer Berlin Heidelberg
Published in: International Journal of Legal Medicine / Issue 5/2018
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-018-1774-z

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Zornia latifolia: a smart drug being adulterated by Stylosanthes guianensis

Abstract

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