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European Archives of Psychiatry and Clinical Neuroscience
Published in: European Archives of Psychiatry and Clinical Neuroscience 8/2012

Open Access 01-12-2012 | Original Paper

Phosphoproteomic differences in major depressive disorder postmortem brains indicate effects on synaptic function

Authors: Daniel Martins-de-Souza, Paul C. Guest, Natacha Vanattou-Saifoudine, Hassan Rahmoune, Sabine Bahn

Published in: European Archives of Psychiatry and Clinical Neuroscience | Issue 8/2012

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Abstract

There is still a lack in the molecular comprehension of major depressive disorder (MDD) although this condition affects approximately 10% of the world population. Protein phosphorylation is a posttranslational modification that regulates approximately one-third of the human proteins involved in a range of cellular and biological processes such as cellular signaling. Whereas phosphoproteome studies have been carried out extensively in cancer research, few such investigations have been carried out in studies of psychiatric disorders. Here, we present a comparative phosphoproteome analysis of postmortem dorsolateral prefrontal cortex tissues from 24 MDD patients and 12 control donors. Tissue extracts were analyzed using liquid chromatography mass spectrometry in a data-independent manner (LC-MSE). Our analyses resulted in the identification of 5,195 phosphopeptides, corresponding to 802 non-redundant proteins. Ninety of these proteins showed differential levels of phosphorylation in tissues from MDD subjects compared to controls, being 20 differentially phosphorylated in at least 2 peptides. The majority of these phosphorylated proteins were associated with synaptic transmission and cellular architecture not only pointing out potential biomarker candidates but mainly shedding light to the comprehension of MDD pathobiology.
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Metadata
Title
Phosphoproteomic differences in major depressive disorder postmortem brains indicate effects on synaptic function
Authors
Daniel Martins-de-Souza
Paul C. Guest
Natacha Vanattou-Saifoudine
Hassan Rahmoune
Sabine Bahn
Publication date
01-12-2012
Publisher
Springer-Verlag
Published in
European Archives of Psychiatry and Clinical Neuroscience / Issue 8/2012
Print ISSN: 0940-1334
Electronic ISSN: 1433-8491
DOI
https://doi.org/10.1007/s00406-012-0301-3

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