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BMC Psychiatry
Published in: BMC Psychiatry 1/2009

Open Access 01-12-2009 | Research article

Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation

Authors: Daniel Martins-de-Souza, Wagner F Gattaz, Andrea Schmitt, José C Novello, Sérgio Marangoni, Christoph W Turck, Emmanuel Dias-Neto

Published in: BMC Psychiatry | Issue 1/2009

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Abstract

Background

Schizophrenia is likely to be a consequence of DNA alterations that, together with environmental factors, will lead to protein expression differences and the ultimate establishment of the illness. The superior temporal gyrus is implicated in schizophrenia and executes functions such as the processing of speech, language skills and sound processing.

Methods

We performed an individual comparative proteome analysis using two-dimensional gel electrophoresis of 9 schizophrenia and 6 healthy control patients' left posterior superior temporal gyrus (Wernicke's area – BA22p) identifying by mass spectrometry several protein expression alterations that could be related to the disease.

Results

Our analysis revealed 11 downregulated and 14 upregulated proteins, most of them related to energy metabolism. Whereas many of the identified proteins have been previously implicated in schizophrenia, such as fructose-bisphosphate aldolase C, creatine kinase and neuron-specific enolase, new putative disease markers were also identified such as dihydrolipoyl dehydrogenase, tropomyosin 3, breast cancer metastasis-suppressor 1, heterogeneous nuclear ribonucleoproteins C1/C2 and phosphate carrier protein, mitochondrial precursor. Besides, the differential expression of peroxiredoxin 6 (PRDX6) and glial fibrillary acidic protein (GFAP) were confirmed by western blot in schizophrenia prefrontal cortex.

Conclusion

Our data supports a dysregulation of energy metabolism in schizophrenia as well as suggests new markers that may contribute to a better understanding of this complex disease.
Appendix
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Metadata
Title
Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation
Authors
Daniel Martins-de-Souza
Wagner F Gattaz
Andrea Schmitt
José C Novello
Sérgio Marangoni
Christoph W Turck
Emmanuel Dias-Neto
Publication date
01-12-2009
Publisher
BioMed Central
Published in
BMC Psychiatry / Issue 1/2009
Electronic ISSN: 1471-244X
DOI
https://doi.org/10.1186/1471-244X-9-17

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