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European Archives of Oto-Rhino-Laryngology

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01-06-2014 | Miscellaneous

Assessment of adenosine deaminase (ADA) activity and oxidative stress in patients with chronic tonsillitis

Authors: Mehmet Fatih Garca, Halit Demir, Mahfuz Turan, Nazım Bozan, Ahmet Kozan, Şeyda Bayel Belli, Ayşe Arslan, Hakan Cankaya

Published in: European Archives of Oto-Rhino-Laryngology | Issue 6/2014

Abstract

To emphasize the effectiveness of adenosine deaminase (ADA) enzyme, which has important roles in the differentiation of lymphoid cells, and oxidative stress in patients with chronic tonsillitis. Serum and tissue samples were obtained from 25 patients who underwent tonsillectomy due to recurrent episodes of acute tonsillitis. In the control group, which also had 25 subjects, only serum samples were taken as obtaining tissue samples would not have been ethically appropriate. ADA enzyme activity, catalase (CAT), carbonic anhydrase (CA), nitric oxide (NO) and malondialdehyde (MDA) were measured in the serum and tissue samples of patients and control group subjects. The serum values of both groups were compared. In addition, the tissue and serum values of patients were compared. Serum ADA activity and the oxidant enzymes MDA and NO values of the patient group were significantly higher than those of the control group (p < 0.001), the antioxidant enzymes CA and CAT values of the patient group were significantly lower than those of the control group (p < 0.001). In addition, while CA, CAT and NO enzyme levels were found to be significantly higher in the tonsil tissue of the patient group when compared to serum levels (p < 0.05), there was no difference between tissue and serum MDA and ADA activity (p > 0.05). Elevated ADA activity may be effective in the pathogenesis of chronic tonsillitis both by impairing tissue structure and contributing to SOR formation.

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Title: Assessment of adenosine deaminase (ADA) activity and oxidative stress in patients with chronic tonsillitis
Authors: Mehmet Fatih Garca
Halit Demir
Mahfuz Turan
Nazım Bozan
Ahmet Kozan
Şeyda Bayel Belli
Ayşe Arslan
Hakan Cankaya
Publication date: 01-06-2014
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Oto-Rhino-Laryngology / Issue 6/2014
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-013-2843-z

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Assessment of adenosine deaminase (ADA) activity and oxidative stress in patients with chronic tonsillitis

Abstract

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Abstract

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