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Archives of Gynecology and Obstetrics

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01-05-2021 | Epigenetics | Gynecologic Endocrinology and Reproductive Medicine

Decreased expression of m⁶A demethylase FTO in ovarian aging

Authors: Xiaoyan Sun, Yigan Zhang, Yuping Hu, Junxia An, Lifei Li, Yiqing Wang, Xuehong Zhang

Published in: Archives of Gynecology and Obstetrics | Issue 5/2021

Abstract

Purpose

N6-methyladenosine (m⁶A) and demethylase fat mass and obesity-associated protein (FTO) were reported to be associated with oocyte development and maturation. But the relationship between FTO and ovarian aging was still unclear. This study was aimed at investigating the FTO expression level and the m⁶A content during ovarian aging.

Methods

The expression level of FTO and the content of m⁶A RNA methylation in human follicular fluid (FF), granulosa cells (GCs) and mouse ovary from different age groups were studied by ELISA, WB, qRT-PCR, IHC and m⁶A Colorimetric.

Results

Human FF ELISA quantified that the level of FTO protein decreased with age (P = 0.025). QRT-PCR results showed that the relative expression of FTO in human GCs was lower in the elderly group than in the young group (P = 0.012). FTO mRNA and protein expression levels were lower in the ovary of 32-week-old mice than in 3- and 8-week-old mice (P < 0.05). Immunohistochemistry showed FTO was relatively decreased in 32-week-old mice (P < 0.05). The m⁶A content in total RNA from old human GCs and ovary from 32-week-old mice was significantly higher compared with the younger ones.

Conclusions

In human FF, GCs and mouse ovary, the expression of FTO decreased while the content of m⁶A increased with aging. However, the inner mechanism still needs further investigation.

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Title: Decreased expression of m6A demethylase FTO in ovarian aging
Authors: Xiaoyan Sun
Yigan Zhang
Yuping Hu
Junxia An
Lifei Li
Yiqing Wang
Xuehong Zhang
Publication date: 01-05-2021
Publisher: Springer Berlin Heidelberg
Keyword: Epigenetics
Published in: Archives of Gynecology and Obstetrics / Issue 5/2021
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-020-05895-7

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Decreased expression of m⁶A demethylase FTO in ovarian aging

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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