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Archives of Gynecology and Obstetrics

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01-09-2019 | Ovarian Cancer | Gynecologic Oncology

Clinical significance and oncogene function of long noncoding RNA HAGLROS overexpression in ovarian cancer

Authors: Meiqin Yang, Zhensheng Zhai, Yunfeng Zhang, Yue Wang

Published in: Archives of Gynecology and Obstetrics | Issue 3/2019

Abstract

Purpose

To explore the clinical significance and mechanism of long noncoding RNA (lncRNA) HAGLROS in ovarian cancer.

Methods

The expression of HAGLROS in ovarian cancer was verified by online databases and quantitative reverse transcription polymerase chain reaction (qRT-PCR), and its relationship with clinicopathological parameters was analysed. Pearson correlation analysis was used to study the correlation between HAGLROS and miR-100 in ovarian cancer. Meta-analysis was used to explore the expression of miR-100 in ovarian cancer. In addition, we used bioinformatics to explore the target genes of miR-100 and perform functional analysis.

Results

HAGLROS was significantly upregulated in ovarian cancer (P < 0.001) and was closely related to disease stage (P = 0.033), tumour size (P = 0.032) and poor prognosis (P = 0.019). HAGLROS had a certain diagnostic value in ovarian cancer (area under the curve = 0.751). MiR-100 was negatively correlated with HAGLROS (r = 0.167, P = 0.001) and significantly downregulated in ovarian cancer. Bioinformatics analysis predicted a total of 31 potential target genes that interact with miR-100. These target genes were mainly involved in the regulation of cellular catabolic process, proteoglycan biosynthetic process and positive regulation of proteasomal ubiquitin-dependent protein catabolic process. Among them, mTOR and ZNRF2 are hub genes.

Conclusion

HAGLROS is a potential biomarker for early diagnosis and prognosis evaluation of ovarian cancer. It can be used as a molecular sponge of miR-100 to regulate the expression of mTOR and ZNRF2 and affect the signal transduction of the mTOR pathway. HAGLROS is expected to be a new target for the treatment of ovarian cancer.

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Title: Clinical significance and oncogene function of long noncoding RNA HAGLROS overexpression in ovarian cancer
Authors: Meiqin Yang
Zhensheng Zhai
Yunfeng Zhang
Yue Wang
Publication date: 01-09-2019
Publisher: Springer Berlin Heidelberg
Keywords: Ovarian Cancer
Ovarian Cancer
Published in: Archives of Gynecology and Obstetrics / Issue 3/2019
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-019-05218-5

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Clinical significance and oncogene function of long noncoding RNA HAGLROS overexpression in ovarian cancer

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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