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Archives of Gynecology and Obstetrics

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01-05-2019 | Endometriosis | Gynecologic Endocrinology and Reproductive Medicine

Examination of cervical swabs of patients with endometriosis using Fourier transform infrared spectroscopy

Authors: Gurkan Bozdag, Nasit Igci, Pinar Calis, Beycan Ayhan, Duygu Ozel Demiralp, Sezcan Mumusoglu, Hakan Yarali

Published in: Archives of Gynecology and Obstetrics | Issue 5/2019

Abstract

Purpose

There is no established non-invasive method to diagnose patients with endometriosis. As a nondestructive type of radiation, infrared light might be used for discrimination by causing vibration of the covalent bonds of the molecules when absorbed by the tissues. The aim of the study was to test whether cervical swab can be used to diagnose women with endometriosis using Fourier transform infrared spectroscopy (FTIR).

Methods

In this prospective case–control study, women between 18–45 years old and undergoing laparoscopy due to various reasons were recruited (n = 20). According to the findings during laparoscopy, patients were stratified as stage I–II or stage III–IV endometriosis groups. Women lacking any visible lesions of endometriosis were recruited as controls. A cervical swab was taken from all patients just before the surgical procedure and pulled into a tube containing saline solution. FTIR spectra were obtained and the fingerprint region (1750–850 cm⁻¹) was used for analyses.

Results

Finally, three samples in stage I–II, five samples in stage III–IV and five samples in the control group were analyzed. Hierarchical cluster analysis and principal component analysis were performed as the chemometric method. A total of ten observable peaks were detected in the absorbance spectra of samples. The peaks at 1450 and 1405 cm⁻¹ originating from lipids and proteins significantly increased in the stage III–IV endometriosis group when compared with controls. In addition, nucleic acid/carbohydrate ratio was significantly lower in the stage I–II group indicating that the alteration of the carbohydrate level might be important.

Conclusions

Examination of cervical swab with FTIR spectroscopy might be a proper candidate for a non-invasive diagnostic approach of endometriosis.

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Title: Examination of cervical swabs of patients with endometriosis using Fourier transform infrared spectroscopy
Authors: Gurkan Bozdag
Nasit Igci
Pinar Calis
Beycan Ayhan
Duygu Ozel Demiralp
Sezcan Mumusoglu
Hakan Yarali
Publication date: 01-05-2019
Publisher: Springer Berlin Heidelberg
Keyword: Endometriosis
Published in: Archives of Gynecology and Obstetrics / Issue 5/2019
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-019-05105-z

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Examination of cervical swabs of patients with endometriosis using Fourier transform infrared spectroscopy

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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