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01-07-2016 | Maternal-Fetal Medicine

Effectiveness and potential mechanisms of intralipid in treating unexplained recurrent spontaneous abortion

Authors: Lili Meng, Jizong Lin, Libin Chen, Zhenhua Wang, Meilan Liu, Yukun Liu, Xin Chen, Liqiong Zhu, Hui Chen, Jianping Zhang

Published in: Archives of Gynecology and Obstetrics | Issue 1/2016

Abstract

Aim

Abnormal natural killer (NK) cell activity has been suggested to be a high-risk factor associated with unexplained recurrent spontaneous abortion (URSA). Intralipid, like immunoglobulin, is able to lower the activity of NK cells, which has been reported to be useful for improving URSA outcomes in pregnancy. This study aimed to determine whether intralipid could be used as an alternative treatment to intravenous immunoglobulin (IVIG) which is expensive and has many side-effects.

Methods

A prospective, randomized clinical trial was conducted from December 2010 to December 2012. Eligible participants were matched and sorted randomly into the intralipid and the IVIG group. The primary outcome was the rate of successful pregnancy. In addition, comparisons of peripheral NK cell activities were accessed by flow cytometry. Moreover, the effects of intralipid on trophoblasts were investigated using a Matrigel assay with the JEG-3 cell line.

Results

Seventy-six patients in the intralipid group and 78 in the IVIG group completed the trial. There were no statistically significant differences in successful pregnancy rates between the two groups (92.1 vs 88.2 %, P = 0.415). The reduced NK cell concentrations revealed the cytotoxic effects of the treatments in both groups. The invasive ability of JEG-3 cells was inhibited during co-culture with patient PBMCs. However, the inhibitory effect could be alleviated if the patient PBMCs were stimulated with intralipid.

Conclusions

Intralipid can be used as an alternative treatment to IVIG for URSA, and its potential mechanism of action may occur by regulating NK cell function and promoting trophoblast invasion.

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Title: Effectiveness and potential mechanisms of intralipid in treating unexplained recurrent spontaneous abortion
Authors: Lili Meng
Jizong Lin
Libin Chen
Zhenhua Wang
Meilan Liu
Yukun Liu
Xin Chen
Liqiong Zhu
Hui Chen
Jianping Zhang
Publication date: 01-07-2016
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 1/2016
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-015-3922-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effectiveness and potential mechanisms of intralipid in treating unexplained recurrent spontaneous abortion

Abstract

Aim

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Aim

Methods

Results

Conclusions

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