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Archives of Gynecology and Obstetrics

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01-03-2013 | General Gynecology

Aberrant histone acetylation and methylation levels in woman with endometriosis

Published in: Archives of Gynecology and Obstetrics | Issue 3/2013

Abstract

Objective

To investigate the alterations in histone modifications in woman with endometriosis.

Methods

Global histone H3/H4 acetylation and H3K4/H3K9 methylation in eutopic and ectopic endometrium from 15 endometriosis patients were assayed using the EpiQuik™ global histone H3/H4 acetylation and H3K4/H3K9 methylation assay kits. Quantitative real-time reverse transcriptase-polymerase chain reaction was applied to measure mRNA levels of 12 members of histone-related chromatin modifier genes.

Results

Histone H4 hypoacetylation was detected both in eutopic and ectopic endometrium. There were no difference between patients with endometriosis and controls on global levels of H3 acetylation. Furthermore, global histone H3K4 hypomethylation and H3K9 hypomethylation were detected both in ectopic and eutopic endometrium (p < 0.001), and in ectopic endometrium (p < 0.001), respectively. SIRT1 mRNA level was significantly decreased in eutopic endometrium, while mRNA levels of HDAC1, SUV39H1, SUV39H2 and G9a were significantly downregulated in ectopic endometrium. HDAC2 mRNA level was significantly increased in eutopic endometrium. PCAF mRNA level was significantly increased in ectopic endometrium.

Conclusions

Aberrant histone modification may play an important role in the pathogenesis of endometriosis.

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Title: Aberrant histone acetylation and methylation levels in woman with endometriosis
Publication date: 01-03-2013
Published in: Archives of Gynecology and Obstetrics / Issue 3/2013
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-012-2591-0

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Aberrant histone acetylation and methylation levels in woman with endometriosis

Abstract

Objective

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objective

Methods

Results

Conclusions

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