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Archives of Gynecology and Obstetrics

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01-06-2012 | Maternal-Fetal Medicine

Alteration in placental expression of bile acids transporters OATP1A2, OATP1B1, OATP1B3 in intrahepatic cholestasis of pregnancy

Authors: Huimin Wang, Ziru Yan, Minyue Dong, Xiaojun Zhu, Hanzhi Wang, Zhengping Wang

Published in: Archives of Gynecology and Obstetrics | Issue 6/2012

Abstract

Purpose

To investigate whether bile acids transporters organic anion transporting polypeptides 1A2 (OATP1A2), organic anion transporting polypeptides 1B1 (OATP1B1), organic anion transporting polypeptides 1B3 (OATP1B3) were differently expressed in placenta of intrahepatic cholestasis of pregnancy (ICP).

Methods

Thirty pregnant women with ICP were recruited and 30 normal pregnant women served as control. The expression of mRNA and protein were analyzed by real-time PCR and Western blotting. The localization of OATP1A2, OATP1B1, OATP1B3 were investigated by immunohistochemistry.

Results

The expression of mRNA and protein of both OATP1A2 and OATP1B3 were significantly lower in ICP placenta than normal placenta (P < 0.05). OATP1B1 mRNA was detected by RT-PCR in 8 ICP placentas and 7 control placentas, but protein expression of OATP1B1 was not found in any of the 60 placentas. By immunohistochemistry we found that OATP1A2 was obviously localized to vasculo-syncytial membrane (VSM) and apical surface of syncytiotrophoblasts, while OATP1B3 was localized to VSM of the syncytiotrophoblasts.

Conclusions

The expression of OATP1A2 and OATP1B3 in placenta decreased in ICP. The down-regulation of these transporters may be involved in the pathophysiology of ICP.

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Title: Alteration in placental expression of bile acids transporters OATP1A2, OATP1B1, OATP1B3 in intrahepatic cholestasis of pregnancy
Authors: Huimin Wang
Ziru Yan
Minyue Dong
Xiaojun Zhu
Hanzhi Wang
Zhengping Wang
Publication date: 01-06-2012
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 6/2012
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-011-2183-4

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Alteration in placental expression of bile acids transporters OATP1A2, OATP1B1, OATP1B3 in intrahepatic cholestasis of pregnancy

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

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