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17-09-2022 | Collagen | Original Paper

Liposomal stem cell extract formulation from Coffea canephora shows outstanding anti-inflammatory activity, increased tissue repair, neocollagenesis and neoangiogenesis

Authors: Marcio Guidoni, Antônio Domingos de Sousa Júnior, Victor Paulo Mesquita Aragão, Thiago de Melo Costa Pereira, Wedson Correa dos Santos, Flavio Cunha Monteiro, Marco Cesar Cunegundes Guimarães, Marcio Fronza

Published in: Archives of Dermatological Research | Issue 3/2023

Abstract

Coffea canephora plant stem cells can have bioactive compounds with tissue repairing and anti-inflammatory action. This study aimed to develop a liposomal stem cell extract formulation obtained from the leaves of C. canephora (LSCECC) and to investigate its capacity to contribute to the dynamic mechanisms of tissue repair. The liposome cream was developed and characterized through the dynamic light scattering technique, atomic force microscopy, and transmission electron microscopy. The excisional full-thickness skin wound model was used and daily topically treated with the LSCECC formulation or vehicle control. On days 2, 7, 14, and 21 after wounding, five rats from each group were euthanized and the rates of wound closure and re-epithelialization were evaluated using biochemical and histological tests. LSCECC resulted in faster re-epithelialization exhibiting a significant reduction in wound area of 36.4, 42.4, and 87.5% after 7, 10, and 14 days, respectively, when compared to vehicle control. LSCECC treated wounds exhibited an increase in granular tissue and a proper inflammatory response mediated by the reduction of pro-inflammatory cytokines like TNF-α and IL-6 and an increase of IL-10. Furthermore, wounds treated with LSCECC showed an increase in the deposition and organization of collagen fibers at the wound site and improved scar tissue quality due to the increase in transforming growth factor-beta and vascular endothelial growth factor. Our data showed that LSCECC improves wound healing, the formation of extracellular matrix, modulates inflammatory response, and promotes neovascularization being consider a promising bioactive extract to promote and support healthy skin.

Graphical abstract

The graphical presents the action of LSCECC in all four phases of wound healing and tissue repair. The LSCECC can reduce the inflammatory infiltrate in the inflammatory phase by decreasing the pro-inflammatory cytokines like IL-6 and TNF-α, in addition to maintaining this modulation through lesser activation and recruitment of macrophages. The LSCECC can also increase the release of IL-10, an anti-inflammatory cytokine, decreasing local edema. The increase in VEGF provides neovascularization and the supply of nutrients to newly repaired tissue. Finally, signaling via TGF-β increases the production and organization of collagen fibers in the remodeling phase.

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Title: Liposomal stem cell extract formulation from Coffea canephora shows outstanding anti-inflammatory activity, increased tissue repair, neocollagenesis and neoangiogenesis
Authors: Marcio Guidoni
Antônio Domingos de Sousa Júnior
Victor Paulo Mesquita Aragão
Thiago de Melo Costa Pereira
Wedson Correa dos Santos
Flavio Cunha Monteiro
Marco Cesar Cunegundes Guimarães
Marcio Fronza
Publication date: 17-09-2022
Publisher: Springer Berlin Heidelberg
Keyword: Collagen
Published in: Archives of Dermatological Research / Issue 3/2023
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-022-02388-2

At a glance: The STEP trials

Springer Medicine

Liposomal stem cell extract formulation from Coffea canephora shows outstanding anti-inflammatory activity, increased tissue repair, neocollagenesis and neoangiogenesis

Abstract

Graphical abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Graphical abstract

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