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Published in:

01-06-2008 | REVIEW

Platelets in regenerative medicine

Authors: Dr. Harald F. Langer, Meinrad Gawaz

Published in: Basic Research in Cardiology | Issue 4/2008

Abstract

Stem and progenitor cells have evolved as the central cell type in regenerative medicine. This focussed approach may, however, sometimes narrow the point of view. Recently, platelets have been shown to strongly influence progenitor cell biology and to serve as regenerative cells themselves. Platelets represent an important recruitment factor for circulating progenitor cells. Furthermore, they modulate progenitor cell chemotaxis, migration and differentiation. Platelets are crucial for the regeneration of parenchymatous organs such as the liver. Moreover, in the clinical setting, platelets are already applied for the restoration of connective and bone tissue. However, also adverse effects can be mediated by platelets and progenitor cells exposed to platelets, including the induction of pro-inflammatory processes. This review gives an update on platelets as regenerative cells and as modulators of progenitor cell biology in (patho-) physiological conditions. Abundant data obtained during the last decade have linked progenitor and stem cells to tissue repair. Very recent accumulating results from different groups revealed that platelets may have a strong influence on central functions of these progenitor cells. Only little is known about the impact of platelets themselves on tissue regeneration. Therefore, this review highlights the role of platelets themselves as a potential effector cell for tissue regeneration and their impact on progenitor cell biology.

Asahara T, Kalka C, Isner JM (2000) Stem cell therapy and gene transfer for regeneration. Gene Ther 7:451–457PubMedCrossRef

Bombeli T, Schwartz BR, Harlan JM (1998) Adhesion of activated platelets to endothelial cells: evidence for a GPIIbIIIa-dependent bridging mechanism and novel roles for endothelial intercellular adhesion molecule 1 (ICAM-1), alphavbeta3 integrin, and GPIbalpha. J Exp Med 187:329–339PubMedCrossRef

Brill A, Elinav H, Varon D (2004) Differential role of platelet granular mediators in angiogenesis. Cardiovasc Res 63:226–235PubMedCrossRef

Buller HR, van Doormaal FF, van Sluis GL, Kamphuisen PW (2007) Cancer and thrombosis: from molecular mechanisms to clinical presentations. J Thromb Haemost 5(Suppl 1):246–254PubMedCrossRef

Burger PC, Wagner DD (2003) Platelet P-selectin facilitates atherosclerotic lesion development. Blood 101:2661–2666PubMedCrossRef

Carter CA, Jolly DG, Worden CE Sr, Hendren DG, Kane CJ (2003) Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp Mol Pathol 74:244–255PubMedCrossRef

Celik S, Langer H, Stellos K, May AE, Shankar V, Kurz K, Katus HA, Gawaz MP, Dengler TJ (2007) Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium. Thromb Haemost 98:798–805PubMed

Daub K, Langer H, Seizer P, Stellos K, May AE, Goyal P, Bigalke B, Schonberger T, Geisler T, Siegel-Axel D, Oostendorp RA, Lindemann S, Gawaz M (2006) Platelets induce differentiation of human CD34+ progenitor cells into foam cells and endothelial cells. FASEB J 20:2559–2561PubMedCrossRef

Daub K, Lindemann S, Langer H, Seizer P, Stellos K, Siegel-Axel D, Gawaz M (2007) The evil in atherosclerosis: adherent platelets induce foam cell formation. Semin Thromb Hemost 33:173–178PubMedCrossRef

10.

de Boer HC, Verseyden C, Ulfman LH, Zwaginga JJ, Bot I, Biessen EA, Rabelink TJ, van Zonneveld AJ (2006) Fibrin and activated platelets cooperatively guide stem cells to a vascular injury and promote differentiation towards an endothelial cell phenotype. Arterioscler Thromb Vasc Biol 26:1653–1659PubMedCrossRef

11.

De Meyer GR, De Cleen DM, Cooper S, Knaapen MW, Jans DM, Martinet W, Herman AG, Bult H, Kockx MM (2002) Platelet phagocytosis and processing of beta-amyloid precursor protein as a mechanism of macrophage activation in atherosclerosis. Circ Res 90:1197–1204PubMedCrossRef

12.

Denis MM, Tolley ND, Bunting M, Schwertz H, Jiang H, Lindemann S, Yost CC, Rubner FJ, Albertine KH, Swoboda KJ, Fratto CM, Tolley E, Kraiss LW, McIntyre TM, Zimmerman GA, Weyrich AS (2005) Escaping the nuclear confines: signal-dependent pre-mRNA splicing in anucleate platelets. Cell 122:379–391PubMedCrossRef

13.

Dong ZM, Chapman SM, Brown AA, Frenette PS, Hynes RO, Wagner DD (1998) The combined role of P- and E-selectins in atherosclerosis. J Clin Invest 102:145–152PubMedCrossRef

14.

Driver VR, Hanft J, Fylling CP, Beriou JM, Autologel Diabetic Foot Ulcer Study Group (2006) A prospective, randomized, controlled trial of autologous platelet-rich plasma gel for the treatment of diabetic foot ulcers. Ostomy Wound Manage 52:68–70, 72, 74PubMed

15.

Du Toit F, Kleintjes WG, Otto MJ, Mazyala EJ, Page BJ (2007) Soft and hard-tissue augmentation with platelet-rich plasma: Tissue culture dynamics, regeneration and molecular biology perspective. Int J Shoulder Surg 1:64–73CrossRef

16.

Fennis JP, Stoelinga PJ, Jansen JA (2004) Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet rich plasma in goats. Int J Oral Maxillofac Surg 33:48–55PubMedCrossRef

17.

Fennis JP, Stoelinga PJ, Jansen JA (2005) Reconstruction of the mandible with an autogenous irradiated cortical scaffold, autogenous corticocancellous bone-graft and autogenous platelet-rich-plasma: an animal experiment. Int J Oral Maxillofac Surg 34:158–166PubMedCrossRef

18.

Fitzgerald JR, Foster TJ, Cox D (2006) The interaction of bacterial pathogens with platelets. Nat Rev Microbiol 4:445–457PubMedCrossRef

19.

Frenette PS, Denis CV, Weiss L, Jurk K, Subbarao S, Kehrel B, Hartwig JH, Vestweber D, Wagner DD (2000) P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo. J Exp Med 191:1413–1422PubMedCrossRef

20.

Frenette PS, Johnson RC, Hynes RO, Wagner DD (1995) Platelets roll on stimulated endothelium in vivo: an interaction mediated by endothelial P-selectin. Proc Natl Acad Sci USA 92:7450–7454PubMedCrossRef

21.

Frenette PS, Moyna C, Hartwell DW, Lowe JB, Hynes RO, Wagner DD (1998) Platelet-endothelial interactions in inflamed mesenteric venules. Blood 91:1318–1324PubMed

22.

Gawaz M (2004) Role of platelets in coronary thrombosis and reperfusion of ischemic myocardium. Cardiovasc Res 61:498–511PubMedCrossRef

23.

Gawaz M, Brand K, Dickfeld T, Pogatsa-Murray G, Page S, Bogner C, Koch W, Schomig A, Neumann F (2000) Platelets induce alterations of chemotactic and adhesive properties of endothelial cells mediated through an interleukin-1-dependent mechanism. Implications for atherogenesis. Atherosclerosis 148:75–85PubMedCrossRef

24.

Gawaz M, Langer H, May AE (2005) Platelets in inflammation and atherogenesis. J Clin Invest 115:3378–3384PubMedCrossRef

25.

Gawaz M, Muller I, Besta F (2003) Combined antithrombotic therapy for acute coronary syndrome. Semin Vasc Med 3:163–176PubMedCrossRef

26.

Gawaz M, Neumann FJ, Dickfeld T, Koch W, Laugwitz KL, Adelsberger H, Langenbrink K, Page S, Neumeier D, Schomig A, Brand K (1998) Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells. Circulation 98:1164–1171PubMed

27.

Gawaz M, Neumann FJ, Dickfeld T, Reininger A, Adelsberger H, Gebhardt A, Schomig A (1997) Vitronectin receptor (alpha(v)beta3) mediates platelet adhesion to the luminal aspect of endothelial cells: implications for reperfusion in acute myocardial infarction. Circulation 96:1809–1818PubMed

28.

Gawaz M, Neumann FJ, Ott I, Schiessler A, Schomig A (1996) Platelet function in acute myocardial infarction treated with direct angioplasty. Circulation 93:229–237PubMed

29.

Gawaz M, Stellos K, Langer HF (2007) Platelets modulate atherogenesis and progression of atherosclerotic plaques via interaction with progenitor and dendritic cells. J Thromb Haemost 6:235–242PubMedCrossRef

30.

Gawaz MP, Loftus JC, Bajt ML, Frojmovic MM, Plow EF, Ginsberg MH (1991) Ligand bridging mediates integrin alpha IIb beta 3 (platelet GPIIB–IIIA) dependent homotypic and heterotypic cell–cell interactions. J Clin Invest 88:1128–1134PubMedCrossRef

31.

Gerard D, Carlson ER, Gotcher JE, Jacobs M (2007) Effects of platelet-rich plasma at the cellular level on healing of autologous bone-grafted mandibular defects in dogs. J Oral Maxillofac Surg 65:721–727PubMedCrossRef

32.

Gruber R, Varga F, Fischer MB, Watzek G (2002) Platelets stimulate proliferation of bone cells: involvement of platelet-derived growth factor, microparticles and membranes. Clin Oral Implants Res 13:529–535PubMedCrossRef

33.

Hagihara M, Higuchi A, Tamura N, Ueda Y, Hirabayashi K, Ikeda Y, Kato S, Sakamoto S, Hotta T, Handa S, Goto S (2004) Platelets, after exposure to a high shear stress, induce IL-10-producing, mature dendritic cells in vitro. J Immunol 172:5297–5303PubMed

34.

Heeschen C, Dimmeler S, Hamm CW, van den Brand MJ, Boersma E, Zeiher AM, Simoons ML (2003) Soluble CD40 ligand in acute coronary syndromes. N Engl J Med 348:1104–1111PubMedCrossRef

35.

Hristov M, Zernecke A, Bidzhekov K, Liehn EA, Shagdarsuren E, Ludwig A, Weber C (2007) Importance of CXC chemokine receptor 2 in the homing of human peripheral blood endothelial progenitor cells to sites of arterial injury. Circ Res 100:590–597PubMedCrossRef

36.

Huo Y, Schober A, Forlow SB, Smith DF, Hyman MC, Jung S, Littman DR, Weber C, Ley K (2003) Circulating activated platelets exacerbate atherosclerosis in mice deficient in apolipoprotein E. Nat Med 9:61–67PubMedCrossRef

37.

Iannacone M, Sitia G, Isogawa M, Marchese P, Castro MG, Lowenstein PR, Chisari FV, Ruggeri ZM, Guidotti LG (2005) Platelets mediate cytotoxic T lymphocyte-induced liver damage. Nat Med 11:1167–1169PubMedCrossRef

38.

Italiano JE Jr, Richardson JL, Patel-Hett S, Battinelli E, Zaslavsky A, Short S, Ryeom S, Folkman J, Klement GL (2007) Angiogenesis is regulated by a novel mechanism: pro- and anti-angiogenic proteins are organized into separate platelet {alpha}-granules and differentialy released. Blood 111:1227–1233PubMedCrossRef

39.

Jans DM, Martinet W, Fillet M, Kockx MM, Merville MP, Bult H, Herman AG, De Meyer GR (2004) Effect of non-steroidal anti-inflammatory drugs on amyloid-beta formation and macrophage activation after platelet phagocytosis. J Cardiovasc Pharmacol 43:462–470PubMedCrossRef

40.

Johnson RC, Mayadas TN, Frenette PS, Mebius RE, Subramaniam M, Lacasce A, Hynes RO, Wagner DD (1995) Blood cell dynamics in P-selectin-deficient mice. Blood 86:1106–1114PubMed

41.

Jorgensen L (2005) ADP-induced platelet aggregation in the microcirculation of pig myocardium and rabbit kidneys. J Thromb Haemost 3:1119–1124PubMedCrossRef

42.

Kalsch T, Elmas E, Nguyen XD, Suvajac N, Kluter H, Borggrefe M, Dempfle CE (2007) Endotoxin-induced effects on platelets and monocytes in an in vivo model of inflammation. Basic Res Cardiol 102:460–466PubMedCrossRef

43.

Kassolis JD, Reynolds MA (2005) Evaluation of the adjunctive benefits of platelet-rich plasma in subantral sinus augmentation. J Craniofac Surg 16:280–287PubMedCrossRef

44.

Kassolis JD, Rosen PS, Reynolds MA (2000) Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freeze-dried bone allograft: case series. J Periodontol 71:1654–1661PubMedCrossRef

45.

Kim SG, Chung CH, Kim YK, Park JC, Lim SC (2002) Use of particulate dentin-plaster of Paris combination with/without platelet-rich plasma in the treatment of bone defects around implants. J Oral Maxillofac Implants 17:86–94

46.

Korporaal SJ, Gorter G, van Rijn HJ, Akkerman JW (2005) Effect of oxidation on the platelet-activating properties of low-density lipoprotein. Arterioscler Thromb Vasc Biol 25:867–872PubMedCrossRef

47.

Kuang D, Zhao X, Xiao G, Ni J, Feng Y, Wu R, Wang G (2007) Stem cell factor/c-kit signaling mediated cardiac stem cell migration via activation of p38 MAPK. Basic Res Cardiol. [Epub ahead of Print]

48.

Langer H, May AE, Bultmann A, Gawaz M (2005) ADAM 15 is an adhesion receptor for platelet GPIIb–IIIa and induces platelet activation. Thromb Haemost 94:555–561PubMed

49.

Langer H, May AE, Daub K, Heinzmann U, Lang P, Schumm M, Vestweber D, Massberg S, Schonberger T, Pfisterer I, Hatzopoulos AK, Gawaz M (2006) Adherent platelets recruit and induce differentiation of murine embryonic endothelial progenitor cells to mature endothelial cells in vitro. Circ Res 98:e2–e10PubMedCrossRef

50.

Langer HF, Daub K, Braun G, Schonberger T, May AE, Schaller M, Stein GM, Stellos K, Bueltmann A, Siegel-Axel D, Wendel HP, Aebert H, Roecken M, Seizer P, Santoso S, Wesselborg S, Brossart P, Gawaz M (2007) Platelets recruit human dendritic cells via Mac-1/JAM-C interaction and modulate dendritic cell function in vitro. Arterioscler Thromb Vasc Biol 27:1463–1470PubMedCrossRef

51.

Langer HF, May AE, Vestweber D, de Boer HC, Hatzopoulos AK, Gawaz M (2007) Platelet-induced differentiation of endothelial progenitor cells. Semin Thromb Hemost 33:136–143PubMedCrossRef

52.

Lesurtel M, Graf R, Aleil B, Walther DJ, Tian Y, Jochum W, Gachet C, Bader M, Clavien PA (2006) Platelet-derived serotonin mediates liver regeneration. Science 312:104–107PubMedCrossRef

53.

Lev EI, Estrov Z, Aboulfatova K, Harris D, Granada JF, Alviar C, Kleiman NS, Dong JF (2006) Potential role of activated platelets in homing of human endothelial progenitor cells to subendothelial matrix. Thromb Haemost 96:498–504PubMed

54.

Lindemann S, Kramer B, Daub K, Stellos K, Gawaz M (2007) Molecular pathways used by platelets to initiate and accelerate atherogenesis. Curr Opin Lipidol 18:566–573PubMed

55.

Lindemann S, Kramer B, Seizer P, Gawaz M (2007) Platelets, inflammation and atherosclerosis. J Thromb Haemost 5(Suppl 1):203–211PubMedCrossRef

56.

Lindemann S, Tolley ND, Dixon DA, McIntyre TM, Prescott SM, Zimmerman GA, Weyrich AS (2001) Activated platelets mediate inflammatory signaling by regulated interleukin 1beta synthesis. J Cell Biol 154:485–490PubMedCrossRef

57.

Lyngbaek S, Schneider M, Hansen JL, Sheikh SP (2007) Cardiac regeneration by resident stem and progenitor cells in the adult heart. Basic Res Cardiol 102:101–114PubMedCrossRef

58.

Maiorana C, Sommariva L, Brivio P, Sigurta D, Santoro F (2003) Maxillary sinus augmentation with anorganic bovine bone (Bio-Oss) and autologous platelet-rich plasma: preliminary clinical and histologic evaluations. Int J Periodontics Restorative Dent 23:227–235PubMed

59.

Manka D, Forlow SB, Sanders JM, Hurwitz D, Bennett DK, Green SA, Ley K, Sarembock IJ (2004) Critical role of platelet P-selectin in the response to arterial injury in apolipoprotein-E-deficient mice. Arterioscler Thromb Vasc Biol 24:1124–1129PubMedCrossRef

60.

Marx RE (2004) Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 62:489–496PubMedCrossRef

61.

Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646PubMedCrossRef

62.

Massberg S, Brand K, Gruner S, Page S, Muller E, Muller I, Bergmeier W, Richter T, Lorenz M, Konrad I, Nieswandt B, Gawaz M (2002) A critical role of platelet adhesion in the initiation of atherosclerotic lesion formation. J Exp Med 196:887–896PubMedCrossRef

63.

Massberg S, Enders G, Leiderer R, Eisenmenger S, Vestweber D, Krombach F, Messmer K (1998) Platelet-endothelial cell interactions during ischemia/reperfusion: the role of P-selectin. Blood 92:507–515PubMed

64.

Massberg S, Enders G, Matos FC, Tomic LI, Leiderer R, Eisenmenger S, Messmer K, Krombach F (1999) Fibrinogen deposition at the postischemic vessel wall promotes platelet adhesion during ischemia-reperfusion in vivo. Blood 94:3829–3838PubMed

65.

Massberg S, Konrad I, Schurzinger K, Lorenz M, Schneider S, Zohlnhoefer D, Hoppe K, Schiemann M, Kennerknecht E, Sauer S, Schulz C, Kerstan S, Rudelius M, Seidl S, Sorge F, Langer H, Peluso M, Goyal P, Vestweber D, Emambokus NR, Busch DH, Frampton J, Gawaz M (2006) Platelets secrete stromal cell-derived factor 1{alpha} and recruit bone marrow-derived progenitor cells to arterial thrombi in vivo. J Exp Med 203:1221–1233PubMedCrossRef

66.

Massberg S, Schurzinger K, Lorenz M, Konrad I, Schulz C, Plesnila N, Kennerknecht E, Rudelius M, Sauer S, Braun S, Kremmer E, Emambokus NR, Frampton J, Gawaz M (2005) Platelet adhesion via glycoprotein IIb integrin is critical for atheroprogression and focal cerebral ischemia: an in vivo study in mice lacking glycoprotein IIb. Circulation 112:1180–1188PubMedCrossRef

67.

Muller I, Kordowich S, Holzwarth C, Spano C, Isensee G, Staiber A, Viebahn S, Gieseke F, Langer H, Gawaz M, Horwitz E, Conte P, Handgretinger R, Dominici M (2006) Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy 8:437–444PubMedCrossRef

68.

Murray MM, Spindler KP, Abreu E, Muller JA, Nedder A, Kelly M, Frino J, Zurakowski D, Valenza M, Snyder BD, Connolly SA (2007) Collagen-platelet rich plasma hydrogel enhances primary repair of the porcine anterior cruciate ligament. J Orthop Res 25:81–91PubMedCrossRef

69.

Murray MM, Spindler KP, Ballard P, Welch TP, Zurakowski D, Nanney LB (2007) Enhanced histologic repair in a central wound in the anterior cruciate ligament with a collagen-platelet-rich plasma scaffold. J Orthop Res 25:1007–1017PubMedCrossRef

70.

Nishimoto S, Oyama T, Matsuda K (2007) Simultaneous concentration of platelets and marrow cells: a simple and useful technique to obtain source cells and growth factors for regenerative medicine. Wound Repair Regen 15:156–162PubMedCrossRef

71.

Robiony M, Polini F, Costa F, Politi M (2002) Osteogenesis distraction and platelet-rich plasma for bone restoration of the severely atrophic mandible: preliminary results. J Oral Maxillofac Surg 60:630–635PubMedCrossRef

72.

Ruggeri ZM (2002) Platelets in atherothrombosis. Nat Med 8:1227–1234PubMedCrossRef

73.

Santoso S, Sachs UJ, Kroll H, Linder M, Ruf A, Preissner KT, Chavakis T (2002) The junctional adhesion molecule 3 (JAM-3) on human platelets is a counterreceptor for the leukocyte integrin Mac-1. J Exp Med 196:679–691PubMedCrossRef

74.

Scheuerer B, Ernst M, Durrbaum-Landmann I, Fleischer J, Grage-Griebenow E, Brandt E, Flad HD, Petersen F (2000) The CXC-chemokine platelet factor 4 promotes monocyte survival and induces monocyte differentiation into macrophages. Blood 95:1158–1166PubMed

75.

Schlegel KA, Donath K, Rupprecht S, Falk S, Zimmermann R, Felszeghy E, Wiltfang J (2004) De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials 25:5387–5393PubMedCrossRef

76.

Schnabel LV, Mohammed HO, Miller BJ, McDermott WG, Jacobson MS, Santangelo KS, Fortier LA (2007) Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res 25:230–240PubMedCrossRef

77.

Schuh A, Liehn EA, Sasse A, Hristov M, Sobota R, Kelm M, Merx MW, Weber C (2008) Transplantation of endothelial progenitor cells improves neovascularization and left ventricular function after myocardial infarction in a rat model. Basic Res Cardiol 103:69–77PubMedCrossRef

78.

Shannon O, Hertzen E, Norrby-Teglund A, Morgelin M, Sjobring U, Bjorck L (2007) Severe streptococcal infection is associated with M protein-induced platelet activation and thrombus formation. Mol Microbiol 65:1147–1157PubMedCrossRef

79.

Shashkin P, Dragulev B, Ley K (2005) Macrophage differentiation to foam cells. Curr Pharm Des 11:3061–3072PubMedCrossRef

80.

Stellos K, Langer H, Daub K, Schönberger T, Gauss A, Geisler T, Bigalke B, Mueller I, Schumm M, Schäfer I, Seizer P, Krämer B, Siegel-Axel D, May AE, Lindemann S, Gawaz M (2007) Platelet-derived SDF-1 regulates adhesion and favors differentiation of human CD34+ progenitor cells to endothelial cells. Circulation [Epub ahead of Print]

81.

Stellos K, Gawaz M (2007) Platelets and stromal cell-derived factor-1 in progenitor cell recruitment. Semin Thromb Hemost 33:159–164PubMedCrossRef

82.

Stellos K, Langer H, Daub K, Schoenberger T, Gauss A, Geisler T, Bigalke B, Mueller I, Schumm M, Schaefer I, Seizer P, Kraemer BF, Siegel-Axel D, May AE, Lindemann S, Gawaz M (2008) Platelet-derived SDF-1 regulates adhesion and promotes differentiation of human CD34+ cells to endothelial progenitor cells. Circulation 117:206–215

83.

Subramaniam M, Frenette PS, Saffaripour S, Johnson RC, Hynes RO, Wagner DD (1996) Defects in hemostasis in P-selectin-deficient mice. Blood 87:1238–1242PubMed

84.

Theilmeier G, Michiels C, Spaepen E, Vreys I, Collen D, Vermylen J, Hoylaerts MF (2002) Endothelial von Willebrand factor recruits platelets to atherosclerosis-prone sites in response to hypercholesterolemia. Blood 99:4486–4493PubMedCrossRef

85.

Thorwarth M, Rupprecht S, Falk S, Felszeghy E, Wiltfang J, Schlegel KA (2005) Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp)-an immunohistochemical analysis. Biomaterials 26:2575–2584PubMedCrossRef

86.

Urbich C, Dimmeler S (2004) Endothelial progenitor cells: characterization and role in vascular biology. Circ Res 95:343–353PubMedCrossRef

87.

van den Dolder J, Mooren R, Vloon AP, Stoelinga PJ, Jansen JA (2006) Platelet-rich plasma: quantification of growth factor levels and the effect on growth and differentiation of rat bone marrow cells. Tissue Eng 12:3067–3073PubMedCrossRef

88.

von Hundelshausen P, Weber KS, Huo Y, Proudfoot AE, Nelson PJ, Ley K, Weber C (2001) RANTES deposition by platelets triggers monocyte arrest on inflamed and atherosclerotic endothelium. Circulation 103:1772–1777

89.

Weber AA, Zucker TP, Schror K (1999) Platelet surface membranes are highly mitogenic for coronary artery smooth muscle cells–A novel mechanism for sustained proliferation after vessel injury? Biochem Biophys Res Commun 259:341–343PubMedCrossRef

90.

Weber C (2005) Platelets and chemokines in atherosclerosis: partners in crime. Circ Res 96:612–616PubMedCrossRef

91.

Weibrich G, Gnoth SH, Otto M, Reichert TE, Wagner W (2002) Growth stimulation of human osteoblast-like cells by thrombocyte concentrates in vitro. Mund Kiefer Gesichtschir 6:168–174PubMedCrossRef

92.

Werner N, Wassmann S, Ahlers P, Schiegl T, Kosiol S, Link A, Walenta K, Nickenig G (2007) Endothelial progenitor cells correlate with endothelial function in patients with coronary artery disease. Basic Res Cardiol 102:565–571PubMedCrossRef

93.

Zernecke A, Schober A, Bot I, von Hundelshausen P, Liehn EA, Mopps B, Mericskay M, Gierschik P, Biessen EA, Weber C (2005) SDF-1alpha/CXCR4 axis is instrumental in neointimal hyperplasia and recruitment of smooth muscle progenitor cells. Circ Res 96:784–791PubMedCrossRef

Title: Platelets in regenerative medicine
Authors: Dr. Harald F. Langer
Meinrad Gawaz
Publication date: 01-06-2008
Publisher: D. Steinkopff-Verlag
Published in: Basic Research in Cardiology / Issue 4/2008
Print ISSN: 0300-8428
Electronic ISSN: 1435-1803
DOI: https://doi.org/10.1007/s00395-008-0721-4

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