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European Journal of Nutrition

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Open Access 01-09-2019 | Original Contribution

Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics

Authors: Jonna Sandberg, Petia Kovatcheva-Datchary, Inger Björck, Fredrik Bäckhed, Anne Nilsson

Published in: European Journal of Nutrition | Issue 6/2019

Abstract

Purpose

We previously showed that short-term intervention with barley kernel bread (BKB) improved glucose tolerance. However, glucose tolerance was not improved in a subset of individuals (non-responders) who were characterized by a low Prevotella/Bacteroides ratio. The purpose of the present study was to investigate if the baseline Prevotella/Bacteroides ratio can be used to stratify metabolic responders and non-responders to barley dietary fiber (DF).

Methods

Fecal samples were collected from 99 healthy humans with BMI < 28 kg/m² between 50 and 70 years old. The abundance of fecal Prevotella and Bacteroides was quantified with 16S rRNA quantitative PCR. 33 subjects were grouped in three groups: subjects with highest Prevotella/Bacteroides ratios, “HP”, n = 12; subjects with lowest Prevotella/Bacteroides ratios, “LP”, n = 13; and subjects with high abundance of both measured bacteria, HPB, n = 8. A 3-day randomized crossover intervention with BKB and white wheat bread (control) was performed. Cardiometabolic test variables were analyzed the next day following a standardized breakfast.

Results

The BKB intervention lowered the blood glucose responses to the breakfast independently of Prevotella/Bacteroides ratios (P < 0.01). However, independently of intervention, the HP group displayed an overall lower insulin response and lower IL-6 concentrations compared with the LP group (P < 0.05). Furthermore, the groups HP and HPB showed lower hunger sensations compared to the LP group (P < 0.05).

Conclusions

Here we show that the abundance of gut Prevotella and Bacteroides at baseline did not stratify metabolic responders and non-responders to barley DF intervention. However, our results indicate the importance of gut microbiota in host metabolic regulation, further suggesting that higher Prevotella/Bacteroides ratio may be favorable.

ClinicalTrials.gov ID

NCT02427555

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Title: Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
Authors: Jonna Sandberg
Petia Kovatcheva-Datchary
Inger Björck
Fredrik Bäckhed
Anne Nilsson
Publication date: 01-09-2019
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 6/2019
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-018-1788-9

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Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics

Abstract

Purpose

Methods

Results

Conclusions

ClinicalTrials.gov ID

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ClinicalTrials.gov ID

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