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01-04-2019 | Original Contribution

Effects of ewe’s milk yogurt (whole and semi-skimmed) and cow’s milk yogurt on inflammation markers and gut microbiota of subjects with borderline-high plasma cholesterol levels: a crossover study

Published in: European Journal of Nutrition | Issue 3/2019

Abstract

Purpose

Ewe’s milk yogurt is richer in proteins, minerals and short- and medium-chain fatty acids compared to cow’s milk yogurt. We aimed to evaluate the effects of cow’s milk yogurt (CW, 3.0% fat), semi-skimmed (ES, 2.8% fat) and whole ewe’s milk yogurts (EW, 5.8% fat) on inflammatory markers and gut microbiota in subjects with borderline-high plasma cholesterol.

Methodology

30 adults (16 women) were randomized into a crossover study to consume 250 g/yogurt/day during three 5-week periods (4-week washouts). Plasma insulin, leptin, adhesion molecules, cytokines and gut microbiota composition (qPCR) were analysed. Rates of change were used to assess treatment effects both in the whole group and in subgroups of subjects with different cholesterol/HDL-c ratio (Cho-I group A: the top 6 women and 4 men values; Cho-I group B: remaining subjects).

Results

The yogurts showed no different effects on the inflammatory biomarkers or the microbiota of the whole group. However, ICAM-1 and P-selectin rates of change were lower after EW compared to CW and ES, respectively, in subjects of the Cho-I group A (P = 0.047 and P = 0.020). Women of this group showed lower MCP-1 rates of change after EW compared to ES and CW (P = 0.028, both). Blautia coccoides–Eubacterium rectale decreased in women of the Cho-I group A during EW vs. ES (P = 0.028).

Conclusion

Ewe’s yogurt effects on inflammatory markers and microbiota were not different from those after cow’s yogurt, but the attenuation of some inflammatory biomarkers with ewe’s whole-milk yogurt in subjects with the highest TC/HDL-c deserves further study.

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Dietary Guidelines Advisory Committee (2015) Scientific report of the 2015 dietary guidelines advisory committee. http://www.health.gov/dietaryguidelines/2015-scientific-report/pdfs/scientific-report-of-the-2015-dietary-guidelines-advisory-committee.pdf. Accessed Oct 2016

Wang H, Livingston KA, Fox CS, Meigs JB, Jacques PF (2013) Yogurt consumption is associated with better diet quality and metabolic profile in American men and women. Nutr Res 33(1):18–26. https://doi.org/10.1016/j.nutres.2012.11.009 CrossRefPubMed

Cormier H, Thifault E, Garneau V, Tremblay A, Drapeau V, Perusse L, Vohl MC (2016) Association between yogurt consumption, dietary patterns, and cardio-metabolic risk factors. Eur J Nutr 55(2):577–587. https://doi.org/10.1007/s00394-015-0878-1 CrossRefPubMed

Adolfsson O, Meydani S, Russell R (2004) Yogurt and gut function. Am J Clin Nutr 80:245–256CrossRefPubMed

Fernandez MA, Panahi S, Daniel N, Tremblay A, Marette A (2017) Yogurt and cardiometabolic diseases: a critical review of potential mechanisms. Adv Nutr 8(6):812–829. https://doi.org/10.3945/an.116.013946 CrossRefPubMedPubMedCentral

Dumas A, Lapointe A, Dugrenier M, Provencher V, Lamarche B, Desroches S (2017) A systematic review of the effect of yogurt consumption on chronic diseases risk markers in adults. Eur J Nutr 56:1375–1392. https://doi.org/10.1007/s00394-016-1341-7 CrossRefPubMed

Sadrzadeh-Yeganeh H, Elmadfa I, Djazayery A, Jalali M, Heshmat R, Chamary M (2010) The effects of probiotic and conventional yoghurt on lipid profile in women. Br J Nutr 103:1778–1783CrossRefPubMed

Labonté ME, Couture P, Richard C, Desroche S, Lamarche B (2013) Impact of dairy products on biomarkers of inflammation: a systematic review of randomized controlled nutritional intervention studies in overweight and obese adults. Am J Clin Nutr 97:706–717CrossRefPubMed

Tamime AY, Robinson RK (1999) Background to manufacturing practice. In: Tamime AY, Robinson RK (eds) Yoghurt science and technology, 2nd edn. CRC Press, Woodhead Publishing Limited, Cambridge, pp 32–33

10.

Jandal JM (1996) Comparative aspects of goat and sheep milk. Small Ruminant Res 22:177–185CrossRef

11.

Katcher HI, Hill AM, Lanford JL, Yoo JS, Kris-Etherton PM (2009) Lifestyle approaches and dietary strategies to lower LDL-cholesterol and triglycerides and raise HDL-cholesterol. Endocrinol Metab Clin North Am 38(1):45–78. https://doi.org/10.1016/j.ecl.2008.11.01. (PubMed PMID:19217512)CrossRefPubMed

12.

Tall AR, Yvan-Charvet L (2015) Cholesterol, inflammation and innate immunity. Nat Rev Immunol 15(2):104–116. https://doi.org/10.1038/nri3793 CrossRefPubMedPubMedCentral

13.

Hansson GK (2005) Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med 352:1685–1695CrossRefPubMed

14.

Calder PC, Ahluwalia N, Albers R, Bosco N, Bourdet-Sicard R, Haller D, Holgate ST, Jönsson LS, Latulippe ME, Marcos A et al (2013) A consideration of biomarkers to be used for evaluation of inflammation in human nutritional studies. Br J Nutr 109(Suppl 1):S1-S34. https://doi.org/10.1017/S0007114512005119 CrossRef

15.

Hogas S, Bilha SC, Branisteanu D, Hogas M, Gaipov A, Kanbay M, Covic A (2017) Potential novel biomarkers of cardiovascular dysfunction and disease: cardiotrophin-1, adipokines and galectin-3. Arch Med Sci 13(4):897–913. https://doi.org/10.5114/aoms.2016.58664 CrossRefPubMed

16.

González-Gil EM, Cadenas-Sanchez C, Santabárbara J, Bueno-Lozano G, Iglesia I, González-Gross M, Molnar D, Gottrand F, De Henauw S, Kafatos A et al; HELENA study group (2018) Inflammation in metabolically healthy and metabolically abnormal adolescents: The HELENA study. Nutr Metab Cardiovasc Dis 28(1):77–83. https://doi.org/10.1016/j.numecd.2017.10.004 CrossRefPubMed

17.

Sonnenburg J, Bäckhed F (2016) Diet–microbiota interactions as moderators of human metabolism. Nature 535(7610):56–64. https://doi.org/10.1038/nature18846 CrossRefPubMedPubMedCentral

18.

German JB, Dillard CJ (2004) Saturated fats: what dietary intake? Am J Clin Nutr 80(3):550–559CrossRefPubMed

19.

Huth P, Fulgoni V, Jandacek RJ, Jones PJ, St-Onge MP, Senanayake V (2010) Bioactivity and emerging role of short and medium chain fatty acids. Lipid Technol 22(12):266–269CrossRef

20.

Azadbakht L, Mirmiran P, Esmaillzadeh A, Azizi F (2005) Dairy consumption is inversely associated with the prevalence of the metabolic syndrome in Tehranian adults. Am J Clin Nutr 82:523–530CrossRefPubMed

21.

Uyeno Y, Sekiguchi Y, Kamagata Y (2008) Impact of consumption of probiotic lactobacilli-containing yogurt on microbial composition in human feces. Int J Food Microbiol 122(1–2):16–22CrossRefPubMed

22.

García-Albiach R, Pozuelo de Felipe M, Angulo S, Morosini MI, Bravo D, Baquero F, del Campo R (2008) Molecular analysis of yogurt containing Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in human intestinal microbiota. Am J Clin Nutr 87:91–96CrossRefPubMed

23.

Lemieux I, Lamarche B, Couillard C, Pascot A, Cantin B, Bergeron J, Dagenais GR, Després JP (2001) Total cholesterol/HDL cholesterol ratio vs LDL cholesterol/HDL cholesterol ratio as indices of ischemic heart disease risk in men: the Quebec Cardiovascular Study. Arch Intern Med 161(22):2685–2892CrossRefPubMed

24.

Klein A, Friedrich U, Vogelsang H, Jahreis G (2008) Lactobacillus acidophilus 74–2 and Bifidobacterium animalis subsp lactis DGCC 420 modulate unspecific cellular immune response in healthy adults. Eur J Clin Nutr 62(5):584–593CrossRefPubMed

25.

Worthley DL, Le Leu RK, Whitehall VL, Conlon M, Christophersen C, Belobrajdic D, Mallitt KA, Hu Y, Irahara N, Ogino S et al (2009) A human, double-blind, placebo-controlled, crossover trial of prebiotic, probiotic, and synbiotic supplementation: effects on luminal, inflammatory, epigenetic, and epithelial biomarkers of colorectal cancer. Am J Clin Nutr 90(3):578–586. https://doi.org/10.3945/ajcn.2009.28106 CrossRefPubMed

26.

Olmedilla-Alonso B, Nova E, García-González N, Martín-Diana AB, Fontecha J, Delgado D, Gredilla AE, Bueno F, Asensio-Vegas C (2017) Effect of ewe’s (semi-skimmed and whole) and cow’s milk-yogurt consumption on the lipid profile of control subjects. A crossover study. Food Nutr Res 61(1):1391669. https://doi.org/10.1080/16546628.2017.1391669 CrossRefPubMedPubMedCentral

27.

Matsuki T, Watanabe K, Fujimoto J, Takada T, Tanaka R (2004) Use of 16S rRna gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Appl Environ Microbiol 70(12):7220–7228CrossRefPubMedPubMedCentral

28.

Gueimonde M, Tölkkö S, Korpimäki T, Salminen S (2004) New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples. Appl Environ Microbiol 70(7):4165–4169CrossRefPubMedPubMedCentral

29.

Bartosch S, Fite A, Macfarlane GT, McMurdo ME (2004) Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota. Appl Environ Microbiol 70(6):3575–3581CrossRefPubMedPubMedCentral

30.

Heilig HGHJ., Zoetendal EG, Vaughan EE, Marteau P, Akkermans ADL, de Vos WM (2002) Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol 68:114e23CrossRef

31.

Rinttilä T, Kassinen A, Malinen E, Krogius L, Palva A (2004) Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 97(6):1166–1177CrossRefPubMed

32.

Savard P, Lamarche B, Paradis ME, Thiboutot H, Laurin É, Roy D (2011) Impact of Bifidobacterium animalis subsp. lactis BB-12 and, Lactobacillus acidophilus LA-5-containing yoghurt, on fecal bacterial counts of healthy adults. Int J Food Microbiol 149(1):50–57CrossRefPubMed

33.

Caesar R, Tremaroli V, Kovatcheva-Datchary P, Cani PD, Bäckhed F (2015) Crosstalk between gut microbiota and dietary lipids aggravates WAT inflammation through TLR signaling. Cell Metab 22(4):658–668. https://doi.org/10.1016/j.cmet.2015.07.026 CrossRefPubMedPubMedCentral

34.

Harvey KA, Walker CL, Pavlina TM, Xu Z, Zaloga GP, Siddiqui RA (2010) Long-chain saturated fatty acids induce pro-inflammatory responses and impact endothelial cell growth. Clin Nutr 29(4):492–500CrossRefPubMed

35.

St-Onge MP, Bosarge A, Goree L, Darnell B (2008) Medium chain triglyceride oil consumption as part of a weight loss diet does Not Lead to an Adverse Metabolic Profile When Compared to Olive Oil. J Am Coll Nutr 27(5):547–552CrossRefPubMedPubMedCentral

36.

Tsuji H, Kasai M, Takeuchi H, Nakamura M, Okazaki M, Kondo K (2001) Dietary medium-chain triacylglycerols suppress accumulation of body fat in a double-blind, controlled trial in healthy men and women. J Nutr 131(11):2853–2859CrossRefPubMed

37.

Zhou S, Wang Y, Jacoby JJ, Jiang Y, Zhang Y, Yu LL (2017) Effects of medium- and long-chain triacylglycerols on lipid metabolism and gut microbiota composition in C57BL/6J Mice. J Agric Food Chem 65(31):6599–6607. https://doi.org/10.1021/acs.jafc.7b01803 CrossRefPubMed

38.

Aoyama T, Nosaka N, Kasai M (2007) Research on the nutritional characteristics of medium-chain fatty acids. J Med Invest 54(3–4):385–388CrossRefPubMed

39.

Papadimitriou C, Vafopoulou-Mastrojiannaki A, Vieira Silva S, Gomes AM, Malcata FX, Alichanidis E (2007) Identification of peptides in traditional and probiotic sheep milk yoghurt with angiotensin I-converting enzyme (ACE)-inhibitory activity. Food Chem 105(2):647–656CrossRef

40.

Shena W, Gaskinsb HR, McIntosha M (2014) Influence of dietary fat on intestinal microbes, inflammation, barrier function and metabolic outcomes. J Nutr Biochem 25:270–280CrossRef

41.

de Wit N, Derrien M, Bosch-Vermeulen H, Oosterink E, Keshtkar S, Duval C, de Vogel-van den Bosch J, Kleerebezem M, Müller M, van der Meer R (2012) Saturated fat stimulates obesity and hepatic steatosis and affects gut microbiota composition by an enhanced overflow of dietary fat to the distal intestine. Am J Physiol Gastrointest Liver Physiol 303(5):G589-G599. https://doi.org/10.1152/ajpgi.00488.2011 CrossRef

42.

Patterson E, O’ Doherty RM, Murphy EF, Wall R, O’ Sullivan O, Nilaweera K, Fitzgerald GF, Cotter PD, Ross RP, Stanton C (2014) Impact of dietary fatty acids on metabolic activity and host intestinal microbiota composition in C57BL/6J mice. Brit J Nutr 20:1–13

43.

Gérard P (2013) Metabolism of cholesterol and bile acids by the gut microbiota. Pathogens 3(1):14–24. https://doi.org/10.3390/pathogens3010014 CrossRefPubMedPubMedCentral

44.

Devkota S, Wang Y, Musch MW, Leone V, Fehlner-Peach H, Nadimpalli A, Antonopoulos DA, Jabri B, Chang EB (2012) Dietary-fat-induced taurocholic acid promotes pathobiont expansion and colitis in Il10–/– mice. Nature 487:104–108CrossRefPubMedPubMedCentral

45.

Forbes JD, Van Domselaar G, Bernstein CN (2016) The gut microbiota in immune-mediated inflammatory diseases. Front Microbiol 7:1081. https://doi.org/10.3389/fmicb.2016.01081 CrossRefPubMedPubMedCentral

46.

Yamashiro K, Tanaka R, Urabe T, Ueno Y, Yamashiro Y, Nomoto K, Takahashi T, Tsuji H, Asahara T, Hattori N (2017) Gut dysbiosis is associated with metabolism and systemic inflammation in patients with ischemic stroke. PLoS One 12(2):e0171521. https://doi.org/10.1371/journal.pone CrossRefPubMedPubMedCentral

47.

Fu J, Bonder MJ, Cenit MC, Tigchelaar EF, Maatman A, Dekens JA, Brandsma E, Marczynska J, Imhann F, Weersma RK et al. (2015) The gut microbiome contributes to a substantial proportion of the variation in blood lipids. Circ Res 117(9):817–824. https://doi.org/10.1161/CIRCRESAHA.115.306807 CrossRefPubMedPubMedCentral

Title: Effects of ewe’s milk yogurt (whole and semi-skimmed) and cow’s milk yogurt on inflammation markers and gut microbiota of subjects with borderline-high plasma cholesterol levels: a crossover study
Publication date: 01-04-2019
Published in: European Journal of Nutrition / Issue 3/2019
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-018-1626-0

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Abstract

Purpose

Methodology

Results

Conclusion

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