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01-02-2019 | Original Contribution

The influence of isoflavone for denervation-induced muscle atrophy

Authors: Shinpei Tabata, Miki Aizawa, Masakazu Kinoshita, Yoshinori Ito, Yusuke Kawamura, Minoru Takebe, Weijun Pan, Kunihiro Sakuma

Published in: European Journal of Nutrition | Issue 1/2019

Abstract

Purpose

Decrease in activity stress induces skeletal muscle atrophy. A previous study showed that treatment with a high level (20%) of isoflavone inhibits muscle atrophy after short-term denervation (at 4 days) in mice. The present study was designed to elucidate whether the dietary isoflavone aglycone (AglyMax) at a 0.6% prevents denervation-mediated muscle atrophy, based on the modulation of atrogin-1- or apoptosis-dependent signaling.

Methods

Mice were fed either a normal diet or 0.6% AglyMax diet. One week later, the right sciatic nerve was cut. The wet weight, mean fiber area, amount of atrogin-1 and cleaved caspase-3 proteins, and the percentages of apoptotic nuclei were examined in the gastrocnemius muscle at 14 days after denervation.

Results

The 0.6% AglyMax diet significantly attenuated denervation-induced decreases in fiber atrophy but not the muscle wet weight. In addition, dietary isoflavone suppressed the denervation-induced apoptosis in spite of there being no significant changes in the amount of cleaved caspase-3 protein. In contrast, the 0.6% AglyMax diet did not significantly modulate the protein expression of atrogin-1 in the denervated muscle of mice.

Conclusions

The isoflavone aglycone (AglyMax) at a 0.6% significantly would modulate muscle atrophy after denervation in mice, probably due to the decrease in apoptosis-dependent signaling.

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Title: The influence of isoflavone for denervation-induced muscle atrophy
Authors: Shinpei Tabata
Miki Aizawa
Masakazu Kinoshita
Yoshinori Ito
Yusuke Kawamura
Minoru Takebe
Weijun Pan
Kunihiro Sakuma
Publication date: 01-02-2019
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 1/2019
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-017-1593-x

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The influence of isoflavone for denervation-induced muscle atrophy

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Purpose

Methods

Results

Conclusions

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