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01-06-2017 | Original Contribution

Effects of intrauterine growth retardation and Bacillus subtilis PB6 supplementation on growth performance, intestinal development and immune function of piglets during the suckling period

Authors: Liang Hu, Xie Peng, Hong Chen, Chuan Yan, Yan Liu, Qin Xu, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Jian Li, De Wu, Lianqiang Che

Published in: European Journal of Nutrition | Issue 4/2017

Abstract

Objectives

The aim of this study was to investigate the effects of intrauterine growth retardation (IUGR) and Bacillus subtilis PB6 supplementation in formula milk (FORM) on growth performance, intestinal development and immune function of neonates using a porcine model.

Methods

Fourteen pairs of normal birth weight and IUGR piglets (7 days old) were randomly assigned to receive FORM or FORM supplemented with B. subtilis PB6 (FORM-BsPB6) for a period of 21 days. Blood samples, intestinal tissues and digesta were collected at necropsy and analysed for morphology, digestive enzyme activities, immune cell abundance, expression of genes associated with innate immunity and barrier function and microbial populations.

Results

Regardless of diet, IUGR significantly decreased average daily dry matter intake and average daily weight gain (P < 0.05). Moreover, IUGR significantly decreased plasma concentrations of immunoglobulin A, interleukin 1β, count and percentage of blood lymphocytes (P < 0.05). Meanwhile, IUGR markedly decreased villous height and maltase activity, as well as mRNA abundance of Toll-like receptor 9 and Toll-interacting protein in the ileum (P < 0.05). Regardless of body weight, FORM-BsPB6 markedly decreased the feed conversion ratio (P < 0.05), due to better intestinal development, as indicated by increased villous height (P < 0.05), activities of maltase and sucrase in the intestine (P < 0.10). Moreover, both mRNA and protein abundances of zonula occludens-1 and claudin-1 in the ileum as well as the copy number of Bacillus in colonic digesta were increased (P < 0.05) in piglets fed FORM-BsPB6 relative to FORM.

Conclusion

The results of this study indicate that IUGR delayed growth, intestinal development and immune function of piglets, while FORM-BsPB6 improved digestive capability and intestinal barrier function.

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Title: Effects of intrauterine growth retardation and Bacillus subtilis PB6 supplementation on growth performance, intestinal development and immune function of piglets during the suckling period
Authors: Liang Hu
Xie Peng
Hong Chen
Chuan Yan
Yan Liu
Qin Xu
Zhengfeng Fang
Yan Lin
Shengyu Xu
Bin Feng
Jian Li
De Wu
Lianqiang Che
Publication date: 01-06-2017
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 4/2017
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-016-1223-z

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Abstract

Objectives

Methods

Results

Conclusion

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