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European Journal of Nutrition

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01-03-2017 | Original Contribution

Ellagic acid in Emblica officinalis exerts anti-diabetic activity through the action on β-cells of pancreas

Authors: Noor Fatima, Rahman M. Hafizur, Abdul Hameed, Shakil Ahmed, Maliha Nisar, Nurul Kabir

Published in: European Journal of Nutrition | Issue 2/2017

Abstract

Purpose

The present study was undertaken to explore the possible anti-diabetic mechanism(s) of Emblica officinalis (EO) and its active constituent, ellagic acid (EA), in vitro and in vivo.

Method

Neonatal streptozotocin-induced non-obese type 2 diabetic rats were treated with a methanolic extract of EO (250 or 500 mg/kg) for 28 days, and blood glucose, serum insulin, and plasma antioxidant status were measured. Insulin and glucagon immunostaining and morphometry were performed in pancreatic section, and liver TBARS and GSH levels were measured. Additionally, EA was tested for glucose-stimulated insulin secretion and glucose tolerance test.

Results

Treatment with EO extract resulted in a significant decrease in the fasting blood glucose in a dose- and time-dependent manner in the diabetic rats. It significantly increased serum insulin in the diabetic rats in a dose-dependent manner. Insulin-to-glucose ratio was also increased by EO treatment. Immunostaining of pancreas showed that EO₂₅₀ increased β-cell size, but EO₅₀₀ increased β-cells number in diabetic rats. EO significantly increased plasma total antioxidants and liver GSH and decreased liver TBARS. EA stimulated glucose-stimulated insulin secretion from isolated islets and decreased glucose intolerance in diabetic rats.

Conclusion

Ellagic acid in EO exerts anti-diabetic activity through the action on β-cells of pancreas that stimulates insulin secretion and decreases glucose intolerance.

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Title: Ellagic acid in Emblica officinalis exerts anti-diabetic activity through the action on β-cells of pancreas
Authors: Noor Fatima
Rahman M. Hafizur
Abdul Hameed
Shakil Ahmed
Maliha Nisar
Nurul Kabir
Publication date: 01-03-2017
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 2/2017
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-015-1103-y

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Conclusion

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