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Open Access 01-08-2016 | Original Contribution

Chronic black tea extract consumption improves endothelial function in ovariectomized rats

Authors: Fung Ping Leung, Lai Ming Yung, Ching Yuen Ngai, Wai San Cheang, Xiao Yu Tian, Chi Wai Lau, Yang Zhang, Jian Liu, Zhen Yu Chen, Zhao-Xiang Bian, Xiaoqiang Yao, Yu Huang

Published in: European Journal of Nutrition | Issue 5/2016

Abstract

Purpose

Menopause escalates the risk of cardiovascular diseases in women. There is an unmet need for better treatment strategy for estrogen-deficiency-related cardiovascular complications. Here we investigated the impact of chronic black tea extract (BT) consumption on cardiovascular function and lipid metabolism using a rat model of estrogen deficiency.

Methods

Female Sprague–Dawley rats were ovariectomized (OVX) and treated with BT (15 mg/kg/day, 4 weeks; active ingredients: theaflavins) or estrogen (E2) treatment for 4 weeks. Serum was collected for measuring cholesterol, triacylglycerol and estradiol levels. Changes in vascular reactivity were examined. The protein levels of NADPH oxidases were assessed by Western blotting. Reactive oxygen species (ROS) level was measured using dihydroethidium fluorescence imaging. The concentrations of cGMP were measured using ELISA kit.

Results

Aortic rings from control, BT-treated and E2-treated OVX rats exhibited a greater increase in Phe-induced contraction after inhibition of NO synthase compared with those from OVX rats. ACh-induced endothelium-dependent relaxations were augmented in aortae and renal arteries in BT/E2-treated OVX rats than in OVX rats. BT/E2 treatment improved flow-mediated dilatation in small mesenteric resistance arteries of OVX rats. BT/E2 treatment restored the eNOS phosphorylation level and reversed the up-regulation of NADPH oxidases and ROS overproduction in OVX rat aortae. ACh-stimulated cGMP production was significantly elevated in the aortae from BT- and E2-treated rats compared with those from OVX rats. BT/E2 treatment reduced circulating levels of total cholesterol.

Conclusions

The present study reveals the novel benefits of chronic BT consumption to reverse endothelial dysfunction and favorably modifying cholesterol profile in a rat model of estrogen deficiency and provides insights into developing BT as beneficial dietary supplements for postmenopausal women.

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Title: Chronic black tea extract consumption improves endothelial function in ovariectomized rats
Authors: Fung Ping Leung
Lai Ming Yung
Ching Yuen Ngai
Wai San Cheang
Xiao Yu Tian
Chi Wai Lau
Yang Zhang
Jian Liu
Zhen Yu Chen
Zhao-Xiang Bian
Xiaoqiang Yao
Yu Huang
Publication date: 01-08-2016
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 5/2016
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-015-1012-0

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